Restriction map for
pF1KB0927
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AfaI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BalI BamHI
BbrPI BbsI BbvCI BbvI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BseRI BsgI BsiEI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspHI BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HincII
HindII HpaI KpnI KspI MaeIII
MamI MfeI MluI MluNI MscI
MseI MslI MunI MvnI NcoI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacII
SalI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
Tru9I TseI Tsp45I Tsp509I Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 239 TCCGGA T^CCGGA
AflIII 339 ACATGT A^C[GA][CT]GT
AspHI 229 GAGCTC G[AT]GC[AT]^C
BanI 103 GGCGCC G^G[CT][GA]CC
BbeI 103 GGCGCC GGCGC^C
BglII 29 AGATCT A^GATCT
BsaWI 239 TCCGGA [AT]^CCGG[AT]
BseAI 239 TCCGGA T^CCGGA
BsiHKAI 229 GAGCTC G[AT]GC[AT]^C
BspEI 239 TCCGGA T^CCGGA
BspLU11I 339 ACATGT A^CATGT
BsrFI 100 GCCGGC [GA]^CCGG[CT]
BstF5I 162 CATCC GGATG(2/0)
BstYI 29 AGATCT [GA]^GATC[CT]
Cfr10I 100 GCCGGC [GA]^CCGG[CT]
DdeI 186 CTTAG C^T[ACGT]AG
EarI 295 GAAGAG CTCTTC(1/4)
EcoNI 111 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 325 CCCGC CCCGC(4/6)
Fnu4HI 206 GCGGC GC^[ACGT]GC
FokI 162 CATCC GGATG(9/13)
HaeII 103 GGCGCC [GA]GCGC^[CT]
HgaI 199 GCGTC GACGC(5/10)
HindIII 183 AAGCTT A^AGCTT
HinfI 71 GATTC G^A[ACGT]TC
HphI 22 GGTGA GGTGA(8/7)
ItaI 206 GCGGC GC^[ACGT]GC
KasI 103 GGCGCC G^GCGCC
Ksp632I 295 GAAGAG CTCTTC(1/4)
MaeII 285 ACGT A^CGT
MflI 29 AGATCT [GA]^GATC[CT]
MroI 239 TCCGGA T^CCGGA
MspA1I 213 CAGCGG C[AC]G^C[GT]G
NaeI 100 GCCGGC GCC^GGC
NarI 103 GGCGCC GG^CGCC
NgoMIV 100 GCCGGC G^CCGGC
NheI 379 GCTAGC G^CTAGC
NspI 339 ACATGT [GA]CATG^[CT]
SacI 229 GAGCTC GAGCT^C
SapI 295 GAAGAGC GCTCTTC(1/4)
SfaNI 58 GATGC GCATC(5/9)
SfoI 103 GGCGCC GGC^GCC
TfiI 71 GATTC G^A[AT]TC
TspRI 311 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 29 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 103 GGCGCC G[GA]^CG[CT]C
198 GGCGTC
AlwI 129 GATCC GGATC(4/5)
242 GGATC
BanII 229 GAGCTC G[GA]GC[CT]^C
362 GAGCCC
BcnI 305 CCCGG CC^[GC]GG
367 CCCGG
BmyI 229 GAGCTC G[AGT]GC[ACT]^C
362 GAGCCC
BpmI 112 CTCCAG CTGGAG(16/14)
290 CTGGAG
BsaHI 103 GGCGCC G[GA]^CG[CT]C
198 GGCGTC
BsaI 218 GAGACC GGTCTC(1/5)
272 GAGACC
Bsp1286I 229 GAGCTC G[AGT]GC[ACT]^C
362 GAGCCC
BsrI 312 ACTGG ACTGG(1/-1)
248 CCAGT
CfoI 104 GCGC GCG^C
352 GCGC
HhaI 104 GCGC GCG^C
352 GCGC
Hin1I 103 GGCGCC G[GA]^CG[CT]C
198 GGCGTC
HinP1I 104 GCGC G^CGC
352 GCGC
MboII 27 GAAGA GAAGA(8/7)
295 GAAGA
NciI 305 CCCGG CC^[GC]GG
367 CCCGG
XcmI 248 CCAGTCTGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
348 CCATGCGCCAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AluI BfaI BsaJI BsiYI
BslI BsmAI BssKI BstNI Cac8I
Cfr13I DpnII EaeI EcoRII HaeIII
HapII HpaII MaeI MboI MnlI
MspI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI