Restriction map for
pF1KB0923
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BglI BglII
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DdeI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HinfI HpaI HphI
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MslI MspA1I MunI NaeI NarI
NciI NdeI NgoMIV NotI NruI
NsiI NspI NspV PacI PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tsp45I TspRI Tth111I XbaI XcmI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 286 CGCG CG^CG
AfaI 342 GTAC GT^AC
AflII 46 CTTAAG C^TTAAG
AlwI 9 GGATC GGATC(4/5)
AlwNI 13 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 290 CTCGAG C^[CT]CG[GA]G
BbvI 329 GCAGC GCAGC(8/12)
BfrI 46 CTTAAG C^TTAAG
BpmI 19 CTGGAG CTGGAG(16/14)
BsaJI 92 CCATGG C^C[ACGT][ACGT]GG
BsmFI 87 GGGAC GGGAC(10/14)
BsoBI 290 CTCGAG C^[CT]CG[GA]G
BsrDI 161 CATTGC GCAATG(2/0)
BsrFI 359 ACCGGC [GA]^CCGG[CT]
BsrI 302 CCAGT ACTGG(1/-1)
BstUI 286 CGCG CG^CG
Cac8I 118 GCTAGC GC[ACGT]^[ACGT]GC
CfoI 287 GCGC GCG^C
Cfr10I 359 ACCGGC [GA]^CCGG[CT]
DpnII 10 GATC ^GATC
DraII 87 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 92 CCATGG C^C[GA][CT]GG
EcoO109I 87 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 92 CCATGG C^C[AT][AT]GG
FauI 312 CCCGC CCCGC(4/6)
Fnu4HI 329 GCAGC GC^[ACGT]GC
HaeIII 228 GGCC GG^CC
HapII 360 CCGG C^CGG
HhaI 287 GCGC GCG^C
HinP1I 287 GCGC G^CGC
HpaII 360 CCGG C^CGG
ItaI 329 GCAGC GC^[ACGT]GC
MaeII 60 ACGT A^CGT
MaeIII 305 GTTAC ^GT[ACGT]AC
MboI 10 GATC ^GATC
MspI 360 CCGG C^CGG
MvnI 286 CGCG CG^CG
MwoI 315 GCCACGAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 92 CCATGG C^CATGG
NdeII 10 GATC ^GATC
NheI 118 GCTAGC G^CTAGC
PaeR7I 290 CTCGAG C^TCGAG
PflMI 92 CCATGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 87 GGGACCC [GA]G^G[AT]CC[CT]
RsaI 342 GTAC GT^AC
SapI 219 GAAGAGC GCTCTTC(1/4)
Sau3AI 10 GATC ^GATC
StyI 92 CCATGG C^C[AT][AT]GG
TaqI 291 TCGA T^CGA
TseI 329 GCAGC G^C[AT]GC
TthHB8I 291 TCGA T^CGA
Van91I 92 CCATGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 290 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AvaII 88 GGACC G^G[AT]CC
353 GGTCC
BfaI 71 CTAG C^TAG
119 CTAG
BsiYI 92 CCATGGGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
356 CCAACCGGCGG
BslI 92 CCATGGGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
356 CCAACCGGCGG
BssKI 225 CCAGG ^CC[ACGT]GG
333 CCAGG
BstF5I 265 CATCC GGATG(2/0)
101 GGATG
BstNI 225 CCAGG CC^[AT]GG
333 CCAGG
EarI 4 GAAGAG CTCTTC(1/4)
219 GAAGAG
EcoRII 225 CCAGG ^CC[AT]GG
333 CCAGG
FokI 265 CATCC GGATG(9/13)
101 GGATG
Ksp632I 4 GAAGAG CTCTTC(1/4)
219 GAAGAG
MaeI 71 CTAG C^TAG
119 CTAG
MvaI 225 CCAGG CC^[AT]GG
333 CCAGG
NlaIII 93 CATG CATG^
187 CATG
ScrFI 225 CCAGG CC^[ACGT]GG
333 CCAGG
SmlI 46 CTTAAG C^T[CT][GA]AG
290 CTCGAG
Tsp509I 141 AATT ^AATT
150 AATT
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
#########################################
AciI AluI Cfr13I MboII MnlI
MseI NlaIV Sau96I Tru9I