Restriction map for
pF1KB0915
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# NO CUT ENZYMES ( 188 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AlwI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbsI
BbvCI BbvI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaBI
BsaHI BseAI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstXI BstYI BstZ17I Bsu36I Cac8I
CelII CfoI ClaI CpoI DpnII
DraI DraII DraIII DrdI DsaI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FokI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MaeIII MamI
MboI MflI MluI MluNI MroI
MscI MseI MslI MspA1I NaeI
NarI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PmeI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI Tru9I
TseI Tsp45I TspRI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 77 CGCG CG^CG
AgeI 100 ACCGGT A^CCGGT
AhdI 96 GACAACCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 168 AGCT AG^CT
Alw44I 177 GTGCAC G^TGCAC
AlwNI 259 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 177 GTGCAC G^TGCAC
AspEI 96 GACAACCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 177 GTGCAC G[AT]GC[AT]^C
AvaII 24 GGACC G^G[AT]CC
BbrPI 41 CACGTG CAC^GTG
BcnI 21 CCGGG CC^[GC]GG
BsaAI 41 CACGTG [CT]AC^GT[GA]
BsaI 103 GGTCTC GGTCTC(1/5)
BsaWI 100 ACCGGT [AT]^CCGG[AT]
BseRI 36 CTCCTC GAGGAG(10/8)
BsiHKAI 177 GTGCAC G[AT]GC[AT]^C
BsmFI 23 GGGAC GGGAC(10/14)
BspHI 107 TCATGA T^CATGA
BsrFI 100 ACCGGT [GA]^CCGG[CT]
BstUI 77 CGCG CG^CG
Cfr10I 100 ACCGGT [GA]^CCGG[CT]
DdeI 265 CTGAG C^T[ACGT]AG
EaeI 193 CGGCCA [CT]^GGCC[GA]
Eam1105I 96 GACAACCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 281 GAAGAG CTCTTC(1/4)
EcoT14I 196 CCAAGG C^C[AT][AT]GG
Fnu4HI 151 GCCGC GC^[ACGT]GC
HgaI 78 GCGTC GACGC(5/10)
HphI 138 TCACC GGTGA(8/7)
ItaI 151 GCCGC GC^[ACGT]GC
Ksp632I 281 GAAGAG CTCTTC(1/4)
MaeII 42 ACGT A^CGT
MfeI 13 CAATTG C^AATTG
MunI 13 CAATTG C^AATTG
MvnI 77 CGCG CG^CG
MwoI 186 GCACATTCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 21 CCGGG CC^[GC]GG
PinAI 100 ACCGGT A^CCGGT
PleI 332 GAGTC GAGTC(4/5)
PmaCI 41 CACGTG CAC^GTG
PmlI 41 CACGTG CAC^GTG
RcaI 107 TCATGA T^CATGA
StuI 51 AGGCCT AGG^CCT
StyI 196 CCAAGG C^C[AT][AT]GG
TfiI 32 GAATC G^A[AT]TC
Tsp509I 14 AATT ^AATT
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# TWO CUT ENZYMES ( 15 enzymes) #
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BsaJI 113 CCCAGG C^C[ACGT][ACGT]GG
196 CCAAGG
BsiYI 38 CCTCACGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
141 CCAAAGACAGG
BslI 38 CCTCACGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
141 CCAAAGACAGG
BstNI 49 CCAGG CC^[AT]GG
114 CCAGG
Cfr13I 24 GGACC G^G[ACGT]CC
46 GGGCC
EcoRII 49 CCAGG ^CC[AT]GG
114 CCAGG
HapII 21 CCGG C^CGG
101 CCGG
HinfI 32 GAATC G^A[ACGT]TC
332 GAGTC
HpaII 21 CCGG C^CGG
101 CCGG
MboII 29 GAAGA GAAGA(8/7)
281 GAAGA
MspI 21 CCGG C^CGG
101 CCGG
MvaI 49 CCAGG CC^[AT]GG
114 CCAGG
NlaIII 108 CATG CATG^
156 CATG
NlaIV 23 GGGACC GG[ACGT]^[ACGT]CC
72 GGAACC
Sau96I 24 GGACC G^G[ACGT]CC
46 GGGCC
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# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
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AciI BmyI BsmAI Bsp1286I BssKI
HaeIII MnlI ScrFI