Restriction map for
pF1KB0491
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AvrII BalI
BamHI BbrPI BbsI BciVI BclI
BfrI BglII BlnI BpuAI BsaAI
BsaBI BsaWI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI Bst1107I BstAPI BstBI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eco81I EcoRI EcoRV
FbaI FseI HincII HindII HindIII
HpaI KpnI MamI MfeI MflI
MluI MluNI MroI MscI MseI
MslI MunI NdeI NheI NotI
NruI NspI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tsp509I XbaI
XhoII XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AhdI 331 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 206 GGATC GGATC(4/5)
Aor51HI 1300 AGCGCT AGC^GCT
ApaI 773 GGGCCC GGGCC^C
AspEI 331 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 1279 GGCGCC G^G[CT][GA]CC
BbeI 1279 GGCGCC GGCGC^C
BbvCI 1683 CCTCAGC CCTCAGC(-5/-2)
BcgI 282 CGAGATTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BspMI 538 ACCTGC ACCTGC(4/8)
BsrDI 546 CATTGC GCAATG(2/0)
BssSI 280 CACGAG CACGAG(-5/-1)
BstEII 321 GGTGACC G^GT[ACGT]ACC
BstPI 321 GGTGACC G^GT[ACGT]ACC
DraIII 421 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 331 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 997 CTCTTC CTCTTC(1/4)
Eco47III 1300 AGCGCT AGC^GCT
EcoO65I 321 GGTGACC G^GT[ACGT]ACC
EcoT22I 1666 ATGCAT ATGCA^T
KasI 1279 GGCGCC G^GCGCC
Ksp632I 997 CTCTTC CTCTTC(1/4)
MboII 998 TCTTC GAAGA(8/7)
NaeI 1306 GCCGGC GCC^GGC
NarI 1279 GGCGCC GG^CGCC
NcoI 1646 CCATGG C^CATGG
NgoMIV 1306 GCCGGC G^CCGGC
NsiI 1666 ATGCAT ATGCA^T
PpuMI 1158 GGGTCCC [GA]G^G[AT]CC[CT]
SapI 996 GCTCTTC GCTCTTC(1/4)
SfiI 1083 GGCCGAAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1279 GGCGCC GGC^GCC
TfiI 177 GATTC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 1253 GGCGTC G[GA]^CG[CT]C
1279 GGCGCC
AfaI 234 GTAC GT^AC
1122 GTAC
AviII 41 TGCGCA TGC^GCA
170 TGCGCA
BlpI 273 GCTGAGC GC^T[ACGT]AGC
1725 GCTGAGC
Bpu1102I 273 GCTGAGC GC^T[ACGT]AGC
1725 GCTGAGC
BsaHI 1253 GGCGTC G[GA]^CG[CT]C
1279 GGCGCC
BsrFI 725 ACCGGC [GA]^CCGG[CT]
1306 GCCGGC
BstF5I 497 CATCC GGATG(2/0)
1669 CATCC
BstXI 551 CCAAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1183 CCAAACCTGTGG
CelII 273 GCTGAGC GC^T[ACGT]AGC
1725 GCTGAGC
Cfr10I 725 ACCGGC [GA]^CCGG[CT]
1306 GCCGGC
DpnII 120 GATC ^GATC
207 GATC
DraII 1158 GGGTCCC [GA]G^G[ACGT]CC[CT]
1169 AGGCCCT
EcoO109I 1158 GGGTCCC [GA]G^G[ACGT]CC[CT]
1169 AGGCCCT
FokI 497 CATCC GGATG(9/13)
1669 CATCC
FspI 41 TGCGCA TGC^GCA
170 TGCGCA
Hin1I 1253 GGCGTC G[GA]^CG[CT]C
1279 GGCGCC
MboI 120 GATC ^GATC
207 GATC
NdeII 120 GATC ^GATC
207 GATC
PaeR7I 73 CTCGAG C^TCGAG
82 CTCGAG
PflMI 108 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
213 CCAGGAGCTGG
PleI 97 GACTC GAGTC(4/5)
1627 GACTC
PvuII 385 CAGCTG CAG^CTG
1579 CAGCTG
RsaI 234 GTAC GT^AC
1122 GTAC
Sau3AI 120 GATC ^GATC
207 GATC
SmaI 1228 CCCGGG CCC^GGG
1365 CCCGGG
SmlI 73 CTCGAG C^T[CT][GA]AG
82 CTCGAG
Van91I 108 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
213 CCAGGAGCTGG
XcmI 1055 CCACGGCCGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1622 CCAGGGACTCCTTGG
XhoI 73 CTCGAG C^TCGAG
82 CTCGAG
XmaCI 1228 CCCGGG C^CCGGG
1365 CCCGGG
XmaI 1228 CCCGGG C^CCGGG
1365 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AluI AlwNI AspHI
AspI AvaI AvaII BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrI BssHII
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DsaI EaeI EagI
EclXI Eco52I EcoNI EcoRII EcoT14I
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI KspI MaeI MaeII
MaeIII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
PflFI PstI SacI SacII Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TseI Tsp45I TspRI Tth111I
TthHB8I