Restriction map for
pF1KB0476
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccII AclI AgeI AhdI
AocI Aor51HI ApaI AscI AspEI
AspI AvrII BbrPI BcgI BclI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspMI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
FbaI FseI HgaI HpaI KspI
MamI MluI MvnI NaeI NgoMIV
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
RsrII SacII SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI Sse8387I
SspBI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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Acc65I 5 GGTACC G^GTACC
AccI 2675 GTCGAC GT^[AC][GT]AC
AccIII 81 TCCGGA T^CCGGA
AcyI 64 GGCGCC G[GA]^CG[CT]C
Alw44I 274 GTGCAC G^TGCAC
AlwNI 2152 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 274 GTGCAC G^TGCAC
AseI 1123 ATTAAT AT^TAAT
AsnI 1123 ATTAAT AT^TAAT
Asp718I 5 GGTACC G^GTACC
AvaI 1100 CCCGAG C^[CT]CG[GA]G
AviII 2468 TGCGCA TGC^GCA
BalI 206 TGGCCA TGG^CCA
BamHI 294 GGATCC G^GATCC
BbeI 64 GGCGCC GGCGC^C
BbvCI 2149 CCTCAGC CCTCAGC(-5/-2)
BsaAI 1202 TACGTG [CT]AC^GT[GA]
BsaHI 64 GGCGCC G[GA]^CG[CT]C
BseAI 81 TCCGGA T^CCGGA
BseRI 1795 CTCCTC GAGGAG(10/8)
BsmFI 655 GGGAC GGGAC(10/14)
BsoBI 1100 CCCGAG C^[CT]CG[GA]G
BspEI 81 TCCGGA T^CCGGA
BspHI 462 TCATGA T^CATGA
BsrBI 51 CCGCTC CCGCTC(-3/-3)
BsrDI 851 CATTGC GCAATG(2/0)
BsrFI 8 ACCGGC [GA]^CCGG[CT]
BstAPI 2833 GCAGTAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 294 GGATCC [GA]^GATC[CT]
Cfr10I 8 ACCGGC [GA]^CCGG[CT]
EaeI 206 TGGCCA [CT]^GGCC[GA]
EcoRI 2989 GAATTC G^AATTC
EcoRV 2803 GATATC GAT^ATC
EcoT22I 1820 ATGCAT ATGCA^T
FspI 2468 TGCGCA TGC^GCA
Hin1I 64 GGCGCC G[GA]^CG[CT]C
HincII 2675 GTCGAC GT[CT]^[GA]AC
HindII 2675 GTCGAC GT[CT]^[GA]AC
HindIII 2591 AAGCTT A^AGCTT
KasI 64 GGCGCC G^GCGCC
KpnI 5 GGTACC GGTAC^C
MfeI 607 CAATTG C^AATTG
MflI 294 GGATCC [GA]^GATC[CT]
MluNI 206 TGGCCA TGG^CCA
MroI 81 TCCGGA T^CCGGA
MscI 206 TGGCCA TGG^CCA
MunI 607 CAATTG C^AATTG
NarI 64 GGCGCC GG^CGCC
NsiI 1820 ATGCAT ATGCA^T
PshBI 1123 ATTAAT AT^TAAT
PvuII 2284 CAGCTG CAG^CTG
RcaI 462 TCATGA T^CATGA
SalI 2675 GTCGAC G^TCGAC
SapI 2427 GCTCTTC GCTCTTC(1/4)
ScaI 222 AGTACT AGT^ACT
SfcI 620 CTACAG C^T[GA][CT]AG
SfoI 64 GGCGCC GGC^GCC
SpeI 424 ACTAGT A^CTAGT
XcmI 2474 CCAAGGAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 294 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 1155 ACATGT A^C[GA][CT]GT
1178 ACATGT
BciVI 1656 GGATAC GTATCC(6/5)
2860 GTATCC
BcnI 1428 CCGGG CC^[GC]GG
2019 CCGGG
BsaWI 81 TCCGGA [AT]^CCGG[AT]
1515 ACCGGA
BsmI 549 GAATGC GAATGC(1/-1)
1251 GAATGC
BspLU11I 1155 ACATGT A^CATGT
1178 ACATGT
BstXI 752 CCAACTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2879 CCAGGGAAATGG
DraII 1023 AGGTCCT [GA]G^G[ACGT]CC[CT]
1212 AGGACCT
EcoNI 1335 CCTAGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2314 CCTGACAGAGG
EcoO109I 1023 AGGTCCT [GA]G^G[ACGT]CC[CT]
1212 AGGACCT
HaeII 64 GGCGCC [GA]GCGC^[CT]
86 AGCGCC
HphI 546 GGTGA GGTGA(8/7)
2034 TCACC
MaeII 1203 ACGT A^CGT
2511 ACGT
NciI 1428 CCGGG CC^[GC]GG
2019 CCGGG
NcoI 2187 CCATGG C^CATGG
2606 CCATGG
NdeI 334 CATATG CA^TATG
1823 CATATG
NheI 12 GCTAGC G^CTAGC
1836 GCTAGC
PleI 599 GACTC GAGTC(4/5)
1804 GACTC
PpuMI 1023 AGGTCCT [GA]G^G[AT]CC[CT]
1212 AGGACCT
SacI 1792 GAGCTC GAGCT^C
2425 GAGCTC
SphI 399 GCATGC GCATG^C
2040 GCATGC
StuI 2138 AGGCCT AGG^CCT
2639 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI ApoI Asp700I AspHI AvaII
BanI BanII BbsI BbvI BfaI
BfrI BmyI BpmI BpuAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EarI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI