Restriction map for
pF1KB0475
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AseI
AsnI Asp700I AspEI AspHI AvaI
AviII AvrII BamHI BanII BbsI
BcgI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaI BsiEI BsiHKAI BsiWI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrGI BssSI Bst1107I
BstAPI BstBI BstZ17I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRV
EcoT22I FbaI FspI HindIII HpaI
KspI MaeI MamI MluI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 361 GTCTAC GT^[AC][GT]AC
AccIII 593 TCCGGA T^CCGGA
AflIII 1181 ACGTGT A^C[GA][CT]GT
AocI 739 CCTGAGG CC^T[ACGT]AGG
AscI 1040 GGCGCGCC GG^CGCGCC
AspI 493 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1180 CACGTG CAC^GTG
BbvCI 518 CCTCAGC CCTCAGC(-5/-2)
BciVI 547 GTATCC GTATCC(6/5)
BcnI 1046 CCGGG CC^[GC]GG
BseAI 593 TCCGGA T^CCGGA
BsmAI 1020 GTCTC GTCTC(1/5)
BsmBI 1019 CGTCTC CGTCTC(1/5)
BsmI 64 GCATTC GAATGC(1/-1)
BspEI 593 TCCGGA T^CCGGA
BssHII 1041 GCGCGC G^CGCGC
BstEII 744 GGTAACC G^GT[ACGT]ACC
BstPI 744 GGTAACC G^GT[ACGT]ACC
BstXI 869 CCAAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 590 AGATCC [GA]^GATC[CT]
Bsu36I 739 CCTGAGG CC^T[ACGT]AGG
DraIII 1289 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 898 CTCTTC CTCTTC(1/4)
Eco81I 739 CCTGAGG CC^T[ACGT]AGG
EcoO65I 744 GGTAACC G^GT[ACGT]ACC
FseI 1281 GGCCGGCC GGCCGG^CC
HincII 682 GTCAAC GT[CT]^[GA]AC
HindII 682 GTCAAC GT[CT]^[GA]AC
Ksp632I 898 CTCTTC CTCTTC(1/4)
MfeI 441 CAATTG C^AATTG
MflI 590 AGATCC [GA]^GATC[CT]
MroI 593 TCCGGA T^CCGGA
MseI 565 TTAA T^TAA
MunI 441 CAATTG C^AATTG
NciI 1046 CCGGG CC^[GC]GG
NcoI 251 CCATGG C^CATGG
PflFI 493 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1180 CACGTG CAC^GTG
PmlI 1180 CACGTG CAC^GTG
PvuII 1480 CAGCTG CAG^CTG
Tru9I 565 TTAA T^TAA
Tth111I 493 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 221 CCAAGCAGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 590 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 454 GGTACC G^GTACC
773 GGTACC
AccII 280 CGCG CG^CG
1042 CGCG
AcsI 292 GAATTC [GA]^AATT[CT]
1003 GAATTC
AlwI 591 GATCC GGATC(4/5)
1118 GGATC
AlwNI 827 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
1240 CAGAACCTG
ApoI 292 GAATTC [GA]^AATT[CT]
1003 GAATTC
Asp718I 454 GGTACC G^GTACC
773 GGTACC
BbeI 22 GGCGCC GGCGC^C
1139 GGCGCC
BmyI 640 GTGCCC G[AGT]GC[ACT]^C
1054 GTGCCC
BpmI 340 CTGGAG CTGGAG(16/14)
346 CTGGAG
BsaAI 1180 CACGTG [CT]AC^GT[GA]
1546 TACGTG
BsaWI 593 TCCGGA [AT]^CCGG[AT]
1448 ACCGGA
Bsp1286I 640 GTGCCC G[AGT]GC[ACT]^C
1054 GTGCCC
BstUI 280 CGCG CG^CG
1042 CGCG
EcoRI 292 GAATTC G^AATTC
1003 GAATTC
HaeII 22 GGCGCC [GA]GCGC^[CT]
1139 GGCGCC
KasI 22 GGCGCC G^GCGCC
1139 GGCGCC
KpnI 454 GGTACC GGTAC^C
773 GGTACC
MvnI 280 CGCG CG^CG
1042 CGCG
NaeI 1142 GCCGGC GCC^GGC
1282 GCCGGC
NarI 22 GGCGCC GG^CGCC
1139 GGCGCC
NgoMIV 1142 GCCGGC G^CCGGC
1282 GCCGGC
PflMI 437 CCACCAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1288 CCACTGGGTGG
SfoI 22 GGCGCC GGC^GCC
1139 GGCGCC
TaqI 399 TCGA T^CGA
471 TCGA
TthHB8I 399 TCGA T^CGA
471 TCGA
Van91I 437 CCACCAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1288 CCACTGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcyI AfaI AluI AvaII
BalI BanI BbvI BsaHI BsaJI
BseRI BsgI BsiYI BslI BsmFI
BspMI BsrFI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TseI Tsp45I Tsp509I TspRI