Restriction map for
pF1KB0455
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspHI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BsrDI BsrGI BssHII
Bst1107I BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoRV EcoT22I FseI FspI HincII
HindII HindIII HpaI KasI KpnI
MaeI MamI MfeI MluI MroI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AcsI 1062 GAATTC [GA]^AATT[CT]
AhdI 1249 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 686 GGGCCC GGGCC^C
ApoI 1062 GAATTC [GA]^AATT[CT]
AspEI 1249 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BclI 518 TGATCA T^GATCA
BsgI 604 GTGCAG GTGCAG(16/14)
BsmFI 224 GGGAC GGGAC(10/14)
BspLU11I 1016 ACATGT A^CATGT
BssSI 877 CACGAG CACGAG(-5/-1)
BstAPI 1458 GCAGTCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 906 GGTCACC G^GT[ACGT]ACC
BstPI 906 GGTCACC G^GT[ACGT]ACC
BstXI 1091 CCAACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 1395 CCGCGG C^C[GA][CT]GG
Eam1105I 1249 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 308 GAAGAG CTCTTC(1/4)
EcoNI 843 CCTGGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 906 GGTCACC G^GT[ACGT]ACC
EcoRI 1062 GAATTC G^AATTC
FbaI 518 TGATCA T^GATCA
HaeII 85 AGCGCC [GA]GCGC^[CT]
Ksp632I 308 GAAGAG CTCTTC(1/4)
KspI 1395 CCGCGG CCGC^GG
MseI 803 TTAA T^TAA
SacII 1395 CCGCGG CCGC^GG
SphI 1358 GCATGC GCATG^C
StuI 1109 AGGCCT AGG^CCT
Tru9I 803 TTAA T^TAA
XcmI 326 CCAGTAACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
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AcyI 100 GACGCC G[GA]^CG[CT]C
1399 GGCGTC
AflIII 1016 ACATGT A^C[GA][CT]GT
1250 ACGTGT
BanI 472 GGTGCC G^G[CT][GA]CC
1299 GGTGCC
BsaHI 100 GACGCC G[GA]^CG[CT]C
1399 GGCGTC
BseRI 143 CTCCTC GAGGAG(10/8)
1432 GAGGAG
BsrFI 433 GCCGGC [GA]^CCGG[CT]
1424 GCCGGC
BstYI 533 AGATCC [GA]^GATC[CT]
704 AGATCC
Cfr10I 433 GCCGGC [GA]^CCGG[CT]
1424 GCCGGC
EcoT14I 911 CCAAGG C^C[AT][AT]GG
1003 CCAAGG
Hin1I 100 GACGCC G[GA]^CG[CT]C
1399 GGCGTC
MaeII 857 ACGT A^CGT
1250 ACGT
MflI 533 AGATCC [GA]^GATC[CT]
704 AGATCC
MspA1I 537 CCGCTG C[AC]G^C[GT]G
1395 CCGCGG
NaeI 433 GCCGGC GCC^GGC
1424 GCCGGC
NgoMIV 433 GCCGGC G^CCGGC
1424 GCCGGC
NspI 1016 ACATGT [GA]CATG^[CT]
1358 GCATGC
PflMI 594 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1193 CCATCAAGTGG
PleI 893 GACTC GAGTC(4/5)
1265 GAGTC
PpuMI 224 GGGACCT [GA]G^G[AT]CC[CT]
257 AGGTCCT
SfaNI 234 GATGC GCATC(5/9)
953 GATGC
StyI 911 CCAAGG C^C[AT][AT]GG
1003 CCAAGG
TfiI 678 GAATC G^A[AT]TC
1148 GAATC
Van91I 594 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1193 CCATCAAGTGG
XhoII 533 AGATCC [GA]^GATC[CT]
704 AGATCC
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaI AvaII BalI BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaJI BsiYI BslI BsmAI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MluNI MnlI
MscI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PstI RsaI Sau3AI Sau96I ScrFI
SfcI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I