Restriction map for
pF1KB6535
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AclI AcyI AfaI
AflII AflIII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BalI
BamHI BbeI BbrPI BbsI BcgI
BclI BfrI BglII BlnI BpuAI
BsaAI BsaBI BsaHI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstF5I BstYI
Cfr10I ClaI CpoI DraI DraII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoRI
EcoRV EcoT22I FbaI FokI FseI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KpnI Ksp632I KspI
MamI MboII MfeI MflI MluI
MluNI MscI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tru9I Tth111I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 382 GTATAC GT^[AC][GT]AC
AccII 489 CGCG CG^CG
AccIII 220 TCCGGA T^CCGGA
AcsI 551 AAATTT [GA]^AATT[CT]
Alw44I 481 GTGCAC G^TGCAC
AocI 986 CCTCAGG CC^T[ACGT]AGG
ApaLI 481 GTGCAC G^TGCAC
ApoI 551 AAATTT [GA]^AATT[CT]
AvaI 557 CCCGGG C^[CT]CG[GA]G
AviII 425 TGCGCA TGC^GCA
BanI 787 GGTGCC G^G[CT][GA]CC
BbvCI 208 CCTCAGC CCTCAGC(-5/-2)
BciVI 163 GGATAC GTATCC(6/5)
BglI 637 GCCACAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 969 GGTCTC GGTCTC(1/5)
BsaWI 220 TCCGGA [AT]^CCGG[AT]
BseAI 220 TCCGGA T^CCGGA
BseRI 908 CTCCTC GAGGAG(10/8)
BsgI 1027 GTGCAG GTGCAG(16/14)
BsoBI 557 CCCGGG C^[CT]CG[GA]G
BspEI 220 TCCGGA T^CCGGA
Bst1107I 382 GTATAC GTA^TAC
BstEII 980 GGTCACC G^GT[ACGT]ACC
BstPI 980 GGTCACC G^GT[ACGT]ACC
BstUI 489 CGCG CG^CG
BstZ17I 382 GTATAC GTA^TAC
Bsu36I 986 CCTCAGG CC^T[ACGT]AGG
DraIII 698 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 503 TGGCCG [CT]^GGCC[GA]
Eco81I 986 CCTCAGG CC^T[ACGT]AGG
EcoO65I 980 GGTCACC G^GT[ACGT]ACC
EcoT14I 157 CCTTGG C^C[AT][AT]GG
FauI 459 CCCGC CCCGC(4/6)
FspI 425 TGCGCA TGC^GCA
HindIII 709 AAGCTT A^AGCTT
MaeII 422 ACGT A^CGT
MroI 220 TCCGGA T^CCGGA
MvnI 489 CGCG CG^CG
NspI 321 GCATGC [GA]CATG^[CT]
PflMI 267 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 765 GAGCTC GAGCT^C
SfcI 131 CTATAG C^T[GA][CT]AG
SmaI 557 CCCGGG CCC^GGG
SphI 321 GCATGC GCATG^C
StyI 157 CCTTGG C^C[AT][AT]GG
TaqI 329 TCGA T^CGA
TfiI 397 GATTC G^A[AT]TC
TthHB8I 329 TCGA T^CGA
Van91I 267 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 557 CCCGGG C^CCGGG
XmaI 557 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 100 GATCC GGATC(4/5)
1082 GGATC
AlwNI 104 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
605 CAGTGTCTG
AspHI 481 GTGCAC G[AT]GC[AT]^C
765 GAGCTC
AvaII 276 GGACC G^G[AT]CC
819 GGACC
BanII 560 GGGCTC G[GA]GC[CT]^C
765 GAGCTC
BfaI 361 CTAG C^TAG
1034 CTAG
BlpI 31 GCTCAGC GC^T[ACGT]AGC
562 GCTCAGC
Bpu1102I 31 GCTCAGC GC^T[ACGT]AGC
562 GCTCAGC
BsiHKAI 481 GTGCAC G[AT]GC[AT]^C
765 GAGCTC
BsmAI 335 GAGAC GTCTC(1/5)
970 GTCTC
BstAPI 428 GCAAGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
721 GCAGAGGTTGC
BstXI 281 CCACCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
806 CCACACCCGTGG
CelII 31 GCTCAGC GC^T[ACGT]AGC
562 GCTCAGC
DpnII 100 GATC ^GATC
1083 GATC
DsaI 377 CCGTGG C^C[GA][CT]GG
812 CCGTGG
HphI 877 TCACC GGTGA(8/7)
982 TCACC
MaeI 361 CTAG C^TAG
1034 CTAG
MboI 100 GATC ^GATC
1083 GATC
NdeII 100 GATC ^GATC
1083 GATC
Sau3AI 100 GATC ^GATC
1083 GATC
SfaNI 296 GATGC GCATC(5/9)
826 GATGC
Tsp45I 733 GTGAC ^GT[GC]AC
981 GTCAC
Tsp509I 535 AATT ^AATT
552 AATT
XcmI 533 CCAATTTTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
806 CCACACCCGTGGTGG
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI BsmFI
Bsp1286I BspMI BsrI BssKI BstNI
Cac8I CfoI Cfr13I DdeI EcoRII
Fnu4HI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeIII MnlI
MslI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV PleI PvuII
Sau96I ScrFI TseI TspRI