Restriction map for
pF1KB8550
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AhdI Alw44I AocI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII BalI BamHI
BcgI BclI BfrI BglII BlpI
Bpu1102I BseAI BsiWI BsmBI Bsp1407I
BspEI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI KspI MfeI MluI
MluNI MroI MscI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I Van91I XbaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 436 GTCTAC GT^[AC][GT]AC
AccII 42 CGCG CG^CG
AgeI 28 ACCGGT A^CCGGT
Aor51HI 2395 AGCGCT AGC^GCT
ApaI 305 GGGCCC GGGCC^C
AspHI 1016 GAGCTC G[AT]GC[AT]^C
AvrII 36 CCTAGG C^CTAGG
BbeI 739 GGCGCC GGCGC^C
BbsI 1582 GTCTTC GAAGAC(2/6)
BglI 1894 GCCAATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 36 CCTAGG C^CTAGG
BpuAI 1582 GTCTTC GAAGAC(2/6)
BsiEI 453 CGACCG CG[GA][CT]^CG
BsiHKAI 1016 GAGCTC G[AT]GC[AT]^C
BsmI 954 GAATGC GAATGC(1/-1)
BspDI 2664 ATCGAT AT^CGAT
BspHI 1547 TCATGA T^CATGA
BsrBI 589 GAGCGG CCGCTC(-3/-3)
BsrFI 28 ACCGGT [GA]^CCGG[CT]
BstUI 42 CGCG CG^CG
Cfr10I 28 ACCGGT [GA]^CCGG[CT]
ClaI 2664 ATCGAT AT^CGAT
Eco47III 2395 AGCGCT AGC^GCT
KasI 739 GGCGCC G^GCGCC
MvnI 42 CGCG CG^CG
NarI 739 GGCGCC GG^CGCC
PaeR7I 824 CTCGAG C^TCGAG
PinAI 28 ACCGGT A^CCGGT
PshAI 516 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1547 TCATGA T^CATGA
SacI 1016 GAGCTC GAGCT^C
SapI 1451 GAAGAGC GCTCTTC(1/4)
SfoI 739 GGCGCC GGC^GCC
StuI 1415 AGGCCT AGG^CCT
XcmI 979 CCAGCTGTTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 824 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcsI 430 AAATTT [GA]^AATT[CT]
724 AAATTC
ApoI 430 AAATTT [GA]^AATT[CT]
724 AAATTC
AvaI 824 CTCGAG C^[CT]CG[GA]G
1397 CCCGAG
BanI 52 GGTGCC G^G[CT][GA]CC
739 GGCGCC
BbrPI 1099 CACGTG CAC^GTG
1345 CACGTG
BbvCI 2406 CCTCAGC CCTCAGC(-5/-2)
901 GCTGAGG
BciVI 486 GGATAC GTATCC(6/5)
550 GGATAC
BsaAI 1099 CACGTG [CT]AC^GT[GA]
1345 CACGTG
BsaBI 1002 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1539 GATCTCCATC
BsaWI 28 ACCGGT [AT]^CCGG[AT]
554 ACCGGA
BsoBI 824 CTCGAG C^[CT]CG[GA]G
1397 CCCGAG
BspMI 510 GCAGGT ACCTGC(4/8)
1026 GCAGGT
BstAPI 1023 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1330 GCAGAGATTGC
BstXI 1340 CCAAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2201 CCATGAAGGTGG
DsaI 1916 CCGTGG C^C[GA][CT]GG
2708 CCACGG
EaeI 788 CGGCCA [CT]^GGCC[GA]
1901 TGGCCG
HaeII 739 GGCGCC [GA]GCGC^[CT]
2395 AGCGCT
HincII 280 GTCAAC GT[CT]^[GA]AC
523 GTCAAC
HindII 280 GTCAAC GT[CT]^[GA]AC
523 GTCAAC
MamI 1002 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1539 GATCTCCATC
NspI 114 ACATGC [GA]CATG^[CT]
284 ACATGC
PleI 242 GAGTC GAGTC(4/5)
2155 GAGTC
PmaCI 1099 CACGTG CAC^GTG
1345 CACGTG
PmlI 1099 CACGTG CAC^GTG
1345 CACGTG
SmlI 824 CTCGAG C^T[CT][GA]AG
1264 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AvaII BanII BbvI BcnI
BfaI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII