Restriction map for
pF1KB8525
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspEI AvrII BamHI
BbrPI BcgI BciVI BfrI BlnI
BsaAI BsaBI BseAI BsiWI BsmI
BspDI BspEI BspHI BspMI BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
EcoNI EcoRI EcoRV EcoT22I FseI
HincII HindII HindIII HpaI MamI
MluI MroI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
XbaI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 2251 GACGTC GACGT^C
Acc65I 125 GGTACC G^GTACC
AcsI 1333 GAATTT [GA]^AATT[CT]
AflIII 1851 ACATGT A^C[GA][CT]GT
ApaI 223 GGGCCC GGGCC^C
ApoI 1333 GAATTT [GA]^AATT[CT]
Asp700I 330 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 125 GGTACC G^GTACC
AspI 2625 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2570 TGCGCA TGC^GCA
BbvCI 760 GCTGAGG CCTCAGC(-5/-2)
BglII 1205 AGATCT A^GATCT
BlpI 2844 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2844 GCTTAGC GC^T[ACGT]AGC
BsaI 2368 GAGACC GGTCTC(1/5)
BspLU11I 1851 ACATGT A^CATGT
BssHII 501 GCGCGC G^CGCGC
BstAPI 2494 GCAGCCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1951 GGTTACC G^GT[ACGT]ACC
BstPI 1951 GGTTACC G^GT[ACGT]ACC
BstXI 749 CCACGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2844 GCTTAGC GC^T[ACGT]AGC
DsaI 569 CCGCGG C^C[GA][CT]GG
EagI 1464 CGGCCG C^GGCCG
EclXI 1464 CGGCCG C^GGCCG
Eco52I 1464 CGGCCG C^GGCCG
EcoO65I 1951 GGTTACC G^GT[ACGT]ACC
FspI 2570 TGCGCA TGC^GCA
KpnI 125 GGTACC GGTAC^C
KspI 569 CCGCGG CCGC^GG
MfeI 1194 CAATTG C^AATTG
MseI 1835 TTAA T^TAA
MunI 1194 CAATTG C^AATTG
NspI 1851 ACATGT [GA]CATG^[CT]
PflFI 2625 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1731 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 735 CTGCAG CTGCA^G
SacI 628 GAGCTC GAGCT^C
SacII 569 CCGCGG CCGC^GG
SexAI 146 ACCAGGT A^CC[AT]GGT
SmlI 631 CTCAAG C^T[CT][GA]AG
StuI 557 AGGCCT AGG^CCT
Tru9I 1835 TTAA T^TAA
Tth111I 2625 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1418 CCATCTGGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 330 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AlwI 661 GATCC GGATC(4/5)
1036 GATCC
AlwNI 279 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1729 CAGACCCTG
AocI 1372 CCTGAGG CC^T[ACGT]AGG
2236 CCTAAGG
BbeI 21 GGCGCC GGCGC^C
2774 GGCGCC
BclI 2400 TGATCA T^GATCA
2579 TGATCA
BfaI 770 CTAG C^TAG
1813 CTAG
BglI 38 GCCCCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1417 GCCATCTGGGC
BsaWI 386 TCCGGT [AT]^CCGG[AT]
2204 ACCGGA
BsgI 1227 CTGCAC GTGCAG(16/14)
2200 CTGCAC
BsiEI 1464 CGGCCG CG[GA][CT]^CG
2838 CGACCG
Bsp1407I 509 TGTACA T^GTACA
1133 TGTACA
BsrBI 2766 CCGCTC CCGCTC(-3/-3)
534 GAGCGG
BsrFI 827 ACCGGC [GA]^CCGG[CT]
2840 ACCGGC
BsrGI 509 TGTACA T^GTACA
1133 TGTACA
BstYI 1035 AGATCC [GA]^GATC[CT]
1205 AGATCT
Bsu36I 1372 CCTGAGG CC^T[ACGT]AGG
2236 CCTAAGG
Cfr10I 827 ACCGGC [GA]^CCGG[CT]
2840 ACCGGC
EarI 28 CTCTTC CTCTTC(1/4)
1542 CTCTTC
Eco81I 1372 CCTGAGG CC^T[ACGT]AGG
2236 CCTAAGG
FbaI 2400 TGATCA T^GATCA
2579 TGATCA
HgaI 1608 GACGC GACGC(5/10)
1143 GCGTC
KasI 21 GGCGCC G^GCGCC
2774 GGCGCC
Ksp632I 28 CTCTTC CTCTTC(1/4)
1542 CTCTTC
MaeI 770 CTAG C^TAG
1813 CTAG
MflI 1035 AGATCC [GA]^GATC[CT]
1205 AGATCT
MslI 720 CATCCTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
750 CACGCTGGTG
NarI 21 GGCGCC GG^CGCC
2774 GGCGCC
SfcI 432 CTGTAG C^T[GA][CT]AG
735 CTGCAG
SfoI 21 GGCGCC GGC^GCC
2774 GGCGCC
SmaI 42 CCCGGG CCC^GGG
374 CCCGGG
SspBI 509 TGTACA T^GTACA
1133 TGTACA
XhoII 1035 AGATCC [GA]^GATC[CT]
1205 AGATCT
XmaCI 42 CCCGGG C^CCGGG
374 CCCGGG
XmaI 42 CCCGGG C^CCGGG
374 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I