Restriction map for
pF1KB8523
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# NO CUT ENZYMES ( 98 enzymes) #
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Acc65I AccII AccIII AclI AfaI
AflII AgeI AscI AseI AsnI
Asp718I AviII AvrII BbeI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BsiEI
BsiWI Bsp1407I BspEI BspHI BsrDI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CelII
Cfr10I CpoI DraI DraIII DrdI
EagI EclXI Eco52I EcoO65I EcoT22I
FbaI FseI FspI KasI KpnI
KspI MamI MluI MroI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflMI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Van91I
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 2320 GACGTC GACGT^C
AcyI 2320 GACGTC G[GA]^CG[CT]C
AhdI 41 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 838 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 2753 AGCGCT AGC^GCT
ApaI 458 GGGCCC GGGCC^C
Asp700I 318 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 41 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1984 GGATCC G^GATCC
BcgI 534 CGAGGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 2320 GACGTC G[GA]^CG[CT]C
BsaI 513 GAGACC GGTCTC(1/5)
BsgI 723 GTGCAG GTGCAG(16/14)
BsmI 439 GCATTC GAATGC(1/-1)
BspDI 1978 ATCGAT AT^CGAT
BssSI 1120 CACGAG CACGAG(-5/-1)
BstXI 578 CCATTGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 2754 GCGC GCG^C
ClaI 1978 ATCGAT AT^CGAT
Eam1105I 41 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2753 AGCGCT AGC^GCT
EcoRI 1878 GAATTC G^AATTC
EcoRV 2591 GATATC GAT^ATC
HaeII 2753 AGCGCT [GA]GCGC^[CT]
HhaI 2754 GCGC GCG^C
Hin1I 2320 GACGTC G[GA]^CG[CT]C
HinP1I 2754 GCGC G^CGC
HindIII 2632 AAGCTT A^AGCTT
HpaI 1129 GTTAAC GTT^AAC
MfeI 1958 CAATTG C^AATTG
MunI 1958 CAATTG C^AATTG
PaeR7I 2075 CTCGAG C^TCGAG
PpuMI 1430 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 262 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1366 CAGCTG CAG^CTG
SmaI 1448 CCCGGG CCC^GGG
XbaI 2511 TCTAGA T^CTAGA
XhoI 2075 CTCGAG C^TCGAG
XmaCI 1448 CCCGGG C^CCGGG
XmaI 1448 CCCGGG C^CCGGG
XmnI 318 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccI 595 GTAGAC GT^[AC][GT]AC
2622 GTCTAC
AcsI 1878 GAATTC [GA]^AATT[CT]
2007 GAATTT
ApoI 1878 GAATTC [GA]^AATT[CT]
2007 GAATTT
AspI 640 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1963 GACCTGGTC
BalI 413 TGGCCA TGG^CCA
656 TGGCCA
BanI 397 GGCACC G^G[CT][GA]CC
2274 GGTGCC
BsmBI 2322 CGTCTC CGTCTC(1/5)
676 GAGACG
BspLU11I 719 ACATGT A^CATGT
2219 ACATGT
BsrBI 2072 CCGCTC CCGCTC(-3/-3)
73 GAGCGG
BstAPI 1313 GCAAAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1937 GCAAGAAATGC
FauI 123 CCCGC CCCGC(4/6)
162 GCGGG
HgaI 678 GACGC GACGC(5/10)
1250 GCGTC
HphI 278 TCACC GGTGA(8/7)
2297 TCACC
MluNI 413 TGGCCA TGG^CCA
656 TGGCCA
MscI 413 TGGCCA TGG^CCA
656 TGGCCA
MspA1I 1278 CCGCTG C[AC]G^C[GT]G
1366 CAGCTG
NspI 719 ACATGT [GA]CATG^[CT]
2219 ACATGT
PflFI 640 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1963 GACCTGGTC
SexAI 170 ACCTGGT A^CC[AT]GGT
1964 ACCTGGT
TaqI 1979 TCGA T^CGA
2076 TCGA
Tth111I 640 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1963 GACCTGGTC
TthHB8I 1979 TCGA T^CGA
2076 TCGA
XcmI 401 CCATGACTGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
909 CCAGTGCTACAGTGG
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# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AflIII AluI Alw44I AlwI
AocI ApaLI AspHI AvaI AvaII
BanII BbrPI BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PmaCI
PmlI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII