Restriction map for
pF1KB5864
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# NO CUT ENZYMES ( 129 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BanI BbeI
BbrPI BbvCI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoT22I
FbaI FseI FspI HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcsI 1131 GAATTT [GA]^AATT[CT]
AcyI 451 GGCGTC G[GA]^CG[CT]C
AfaI 921 GTAC GT^AC
ApaI 68 GGGCCC GGGCC^C
ApoI 1131 GAATTT [GA]^AATT[CT]
AvaII 114 GGACC G^G[AT]CC
BamHI 428 GGATCC G^GATCC
BbsI 512 GAAGAC GAAGAC(2/6)
BcgI 51 CGAGCTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 61 GCCCGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 782 AGATCT A^GATCT
BpuAI 512 GAAGAC GAAGAC(2/6)
BsaHI 451 GGCGTC G[GA]^CG[CT]C
BsaWI 873 TCCGGT [AT]^CCGG[AT]
BseRI 372 CTCCTC GAGGAG(10/8)
BsgI 460 GTGCAG GTGCAG(16/14)
BsmBI 639 GAGACG CGTCTC(1/5)
BsmI 1452 GCATTC GAATGC(1/-1)
BspHI 945 TCATGA T^CATGA
BspMI 107 ACCTGC ACCTGC(4/8)
EcoNI 1014 CCTTTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 451 GGCGTC G[GA]^CG[CT]C
HincII 573 GTTGAC GT[CT]^[GA]AC
HindII 573 GTTGAC GT[CT]^[GA]AC
HindIII 904 AAGCTT A^AGCTT
MaeII 1049 ACGT A^CGT
NcoI 447 CCATGG C^CATGG
PleI 370 GACTC GAGTC(4/5)
PstI 37 CTGCAG CTGCA^G
RcaI 945 TCATGA T^CATGA
RsaI 921 GTAC GT^AC
SacI 52 GAGCTC GAGCT^C
SfcI 37 CTGCAG C^T[GA][CT]AG
SmaI 4 CCCGGG CCC^GGG
StuI 255 AGGCCT AGG^CCT
TaqI 1028 TCGA T^CGA
TthHB8I 1028 TCGA T^CGA
XmaCI 4 CCCGGG C^CCGGG
XmaI 4 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AlwNI 31 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
40 CAGCTGCTG
AspHI 52 GAGCTC G[AT]GC[AT]^C
76 GAGCAC
AvaI 4 CCCGGG C^[CT]CG[GA]G
1285 CCCGAG
BfaI 346 CTAG C^TAG
982 CTAG
BpmI 73 CTGGAG CTGGAG(16/14)
1407 CTGGAG
BsiHKAI 52 GAGCTC G[AT]GC[AT]^C
76 GAGCAC
BsoBI 4 CCCGGG C^[CT]CG[GA]G
1285 CCCGAG
BsrDI 1440 CATTGC GCAATG(2/0)
1141 GCAATG
BstF5I 1011 CATCC GGATG(2/0)
1223 CATCC
DraI 681 TTTAAA TTT^AAA
1134 TTTAAA
DraII 67 GGGGCCC [GA]G^G[ACGT]CC[CT]
68 GGGCCCT
EaeI 47 TGGCCG [CT]^GGCC[GA]
957 CGGCCA
EarI 211 CTCTTC CTCTTC(1/4)
1212 GAAGAG
EcoO109I 67 GGGGCCC [GA]G^G[ACGT]CC[CT]
68 GGGCCCT
EcoRV 145 GATATC GAT^ATC
1024 GATATC
EcoT14I 447 CCATGG C^C[AT][AT]GG
746 CCAAGG
FauI 742 CCCGC CCCGC(4/6)
111 GCGGG
FokI 1011 CATCC GGATG(9/13)
1223 CATCC
HaeII 33 GGCGCT [GA]GCGC^[CT]
464 AGCGCC
HgaI 16 GACGC GACGC(5/10)
452 GCGTC
HphI 27 TCACC GGTGA(8/7)
1037 TCACC
Ksp632I 211 CTCTTC CTCTTC(1/4)
1212 GAAGAG
MaeI 346 CTAG C^TAG
982 CTAG
MslI 1356 CATCGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1405 CACTGGAGTG
MspA1I 40 CAGCTG C[AC]G^C[GT]G
84 CAGCTG
PflMI 960 CCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1090 CCAACGGCTGG
PvuII 40 CAGCTG CAG^CTG
84 CAGCTG
SfaNI 1010 GCATC GCATC(5/9)
1159 GCATC
StyI 447 CCATGG C^C[AT][AT]GG
746 CCAAGG
TfiI 720 GATTC G^A[AT]TC
1245 GAATC
Van91I 960 CCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1090 CCAACGGCTGG
XcmI 1040 CCAATGACAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1313 CCATAGCAGTCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AluI AlwI BanII
BbvI BcnI BmyI BsaI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EcoRII Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeIII MboI MboII MflI MnlI
MseI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII