Restriction map for
pF1KB5858
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccI AccIII AcyI
AflII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AvaI
AviII BalI BamHI BbeI BcgI
BfrI BglI BsaBI BsaHI BsaI
BsaWI BseAI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BsrFI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoT22I FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HinfI
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PleI PmeI PshAI PshBI PvuI
RsrII SacI SacII SalI SapI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SspBI
SspI SwaI TfiI Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AclI 769 AACGTT AA^CGTT
AcsI 1363 GAATTC [GA]^AATT[CT]
AflIII 823 ACATGT A^C[GA][CT]GT
ApaI 710 GGGCCC GGGCC^C
ApoI 1363 GAATTC [GA]^AATT[CT]
Asp700I 1359 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 409 GAGCAC G[AT]GC[AT]^C
AspI 744 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 714 CCTAGG C^CTAGG
BbrPI 403 CACGTG CAC^GTG
BbsI 31 GTCTTC GAAGAC(2/6)
BbvCI 40 GCTGAGG CCTCAGC(-5/-2)
BciVI 930 GGATAC GTATCC(6/5)
BclI 555 TGATCA T^GATCA
BfaI 715 CTAG C^TAG
BglII 722 AGATCT A^GATCT
BlnI 714 CCTAGG C^CTAGG
BpuAI 31 GTCTTC GAAGAC(2/6)
BsaAI 403 CACGTG [CT]AC^GT[GA]
BsiEI 222 CGACCG CG[GA][CT]^CG
BsiHKAI 409 GAGCAC G[AT]GC[AT]^C
BspLU11I 823 ACATGT A^CATGT
BsrBI 106 GAGCGG CCGCTC(-3/-3)
BsrDI 1433 GCAATG GCAATG(2/0)
BssHII 322 GCGCGC G^CGCGC
BstYI 722 AGATCT [GA]^GATC[CT]
EarI 859 CTCTTC CTCTTC(1/4)
EcoRI 1363 GAATTC G^AATTC
EcoRV 91 GATATC GAT^ATC
FbaI 555 TGATCA T^GATCA
Ksp632I 859 CTCTTC CTCTTC(1/4)
MaeI 715 CTAG C^TAG
MflI 722 AGATCT [GA]^GATC[CT]
PflFI 744 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 403 CACGTG CAC^GTG
PmlI 403 CACGTG CAC^GTG
Psp1406I 769 AACGTT AA^CGTT
PvuII 793 CAGCTG CAG^CTG
SbfI 567 CCTGCAGG CCTGCA^GG
Sse8387I 567 CCTGCAGG CCTGCA^GG
TaqI 758 TCGA T^CGA
Tsp45I 1273 GTGAC ^GT[GC]AC
Tth111I 744 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 758 TCGA T^CGA
XhoII 722 AGATCT [GA]^GATC[CT]
XmnI 1359 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccII 323 CGCG CG^CG
1384 CGCG
BanI 294 GGTGCC G^G[CT][GA]CC
352 GGCACC
BcnI 356 CCGGG CC^[GC]GG
996 CCGGG
BlpI 103 GCTGAGC GC^T[ACGT]AGC
1059 GCTGAGC
BpmI 1090 CTCCAG CTGGAG(16/14)
1006 CTGGAG
Bpu1102I 103 GCTGAGC GC^T[ACGT]AGC
1059 GCTGAGC
BsgI 819 CTGCAC GTGCAG(16/14)
1324 GTGCAG
BspHI 660 TCATGA T^CATGA
975 TCATGA
BstUI 323 CGCG CG^CG
1384 CGCG
CelII 103 GCTGAGC GC^T[ACGT]AGC
1059 GCTGAGC
DsaI 383 CCGTGG C^C[GA][CT]GG
575 CCGTGG
HapII 356 CCGG C^CGG
996 CCGG
HpaII 356 CCGG C^CGG
996 CCGG
MaeIII 1220 GTAAC ^GT[ACGT]AC
1273 GTGAC
MspA1I 38 CCGCTG C[AC]G^C[GT]G
793 CAGCTG
MspI 356 CCGG C^CGG
996 CCGG
MvnI 323 CGCG CG^CG
1384 CGCG
NciI 356 CCGGG CC^[GC]GG
996 CCGGG
PpuMI 434 AGGACCT [GA]G^G[AT]CC[CT]
1076 AGGTCCT
PstI 86 CTGCAG CTGCA^G
568 CTGCAG
RcaI 660 TCATGA T^CATGA
975 TCATGA
ScaI 467 AGTACT AGT^ACT
806 AGTACT
SfcI 86 CTGCAG C^T[GA][CT]AG
568 CTGCAG
SphI 497 GCATGC GCATG^C
1212 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AvaII BanII
BbvI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HhaI HinP1I HphI
ItaI MaeII MboI MboII MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
Sau96I ScrFI SfaNI StuI StyI
Tru9I TseI Tsp509I TspRI XcmI