Restriction map for
pF1KB7895
##################################
# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AgeI AhdI Alw44I AocI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspHI AspI AviII
AvrII BalI BamHI BanII BbeI
BbvCI BciVI BclI BfrI BlnI
BlpI BmyI BpmI Bpu1102I BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmFI BsmI
Bsp1286I BspDI BspEI BspHI BspMI
BsrDI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstNI BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraII DraIII DrdI
EaeI EagI Eam1105I EclXI Eco52I
Eco81I EcoO109I EcoO65I EcoRII FauI
FbaI FseI FspI HgaI Hin1I
HpaI KasI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvaI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I Van91I
XbaI XcmI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 1208 GGTACC G^GTACC
AccI 1312 GTCGAC GT^[AC][GT]AC
AlwNI 1391 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 329 AGCGCT AGC^GCT
Asp718I 1208 GGTACC G^GTACC
AvaI 7 CTCGAG C^[CT]CG[GA]G
BanI 1208 GGTACC G^G[CT][GA]CC
BbrPI 979 CACGTG CAC^GTG
BbsI 892 GAAGAC GAAGAC(2/6)
BcgI 1373 GCACACTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 177 CCGGG CC^[GC]GG
BfaI 434 CTAG C^TAG
BglI 1036 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 696 AGATCT A^GATCT
BpuAI 892 GAAGAC GAAGAC(2/6)
BsaAI 979 CACGTG [CT]AC^GT[GA]
BseRI 10 GAGGAG GAGGAG(10/8)
BsmBI 1755 GAGACG CGTCTC(1/5)
BsoBI 7 CTCGAG C^[CT]CG[GA]G
BspLU11I 1575 ACATGT A^CATGT
BsrBI 488 GAGCGG CCGCTC(-3/-3)
BssKI 177 CCGGG ^CC[ACGT]GG
BstAPI 92 GCACTTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 330 GCGC GCG^C
DraI 25 TTTAAA TTT^AAA
Eco47III 329 AGCGCT AGC^GCT
EcoNI 6 CCTCGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1524 GAATTC G^AATTC
EcoRV 119 GATATC GAT^ATC
EcoT22I 961 ATGCAT ATGCA^T
HaeII 329 AGCGCT [GA]GCGC^[CT]
HapII 177 CCGG C^CGG
HhaI 330 GCGC GCG^C
HinP1I 330 GCGC G^CGC
HindIII 1226 AAGCTT A^AGCTT
HpaII 177 CCGG C^CGG
HphI 1350 GGTGA GGTGA(8/7)
KpnI 1208 GGTACC GGTAC^C
MaeI 434 CTAG C^TAG
MslI 1083 CATTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 462 CAGCTG C[AC]G^C[GT]G
MspI 177 CCGG C^CGG
NciI 177 CCGGG CC^[GC]GG
NlaIV 1208 GGTACC GG[ACGT]^[ACGT]CC
NsiI 961 ATGCAT ATGCA^T
NspI 1575 ACATGT [GA]CATG^[CT]
PaeR7I 7 CTCGAG C^TCGAG
PmaCI 979 CACGTG CAC^GTG
PmlI 979 CACGTG CAC^GTG
PstI 1557 CTGCAG CTGCA^G
PvuII 462 CAGCTG CAG^CTG
SalI 1312 GTCGAC G^TCGAC
SapI 342 GAAGAGC GCTCTTC(1/4)
ScrFI 177 CCGGG CC^[ACGT]GG
SmlI 7 CTCGAG C^T[CT][GA]AG
SspI 83 AATATT AAT^ATT
XhoI 7 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 16 GCGG CCGC(-3/-1)
490 GCGG
AcsI 1524 GAATTC [GA]^AATT[CT]
1552 AAATTC
AflIII 1459 ACGTGT A^C[GA][CT]GT
1575 ACATGT
ApoI 1524 GAATTC [GA]^AATT[CT]
1552 AAATTC
AvaII 3 GGACC G^G[AT]CC
720 GGACC
BbvI 49 GCTGC GCAGC(8/12)
447 GCTGC
Bsp1407I 1065 TGTACA T^GTACA
1570 TGTACA
BsrGI 1065 TGTACA T^GTACA
1570 TGTACA
Cac8I 347 GCAAGC GC[ACGT]^[ACGT]GC
351 GCAGGC
DsaI 1186 CCATGG C^C[GA][CT]GG
1467 CCATGG
Fnu4HI 49 GCTGC GC^[ACGT]GC
447 GCTGC
HincII 943 GTCAAC GT[CT]^[GA]AC
1312 GTCGAC
HindII 943 GTCAAC GT[CT]^[GA]AC
1312 GTCGAC
ItaI 49 GCTGC GC^[ACGT]GC
447 GCTGC
NcoI 1186 CCATGG C^CATGG
1467 CCATGG
SfaNI 1562 GATGC GCATC(5/9)
541 GCATC
SspBI 1065 TGTACA T^GTACA
1570 TGTACA
TseI 49 GCTGC G^C[AT]GC
447 GCTGC
Tsp45I 782 GTGAC ^GT[GC]AC
1579 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AfaI AluI AlwI BsaJI BsiYI
BslI BsmAI BsrI BstF5I BstYI
Cfr13I DdeI DpnII EarI EcoT14I
FokI HaeIII HinfI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MseI MwoI NdeII NlaIII PleI
RsaI Sau3AI Sau96I SfcI StyI
TaqI TfiI Tru9I Tsp509I TspRI
TthHB8I XhoII