Restriction map for
pF1KB7784
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BcgI BciVI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BseAI BseRI BsiWI
BsmBI BsoBI BspDI BspEI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
DsaI EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoT22I
FauI FseI FspI HgaI Hin1I
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 21 CGCG CG^CG
AflIII 1432 ACATGT A^C[GA][CT]GT
Aor51HI 139 AGCGCT AGC^GCT
Asp700I 1113 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 442 GTGCTC G[AT]GC[AT]^C
BanII 493 GAGCCC G[GA]GC[CT]^C
BbsI 768 GAAGAC GAAGAC(2/6)
BbvCI 231 GCTGAGG CCTCAGC(-5/-2)
BbvI 1422 GCAGC GCAGC(8/12)
BcnI 540 CCGGG CC^[GC]GG
BpuAI 768 GAAGAC GAAGAC(2/6)
BsaI 310 GAGACC GGTCTC(1/5)
BsaWI 181 ACCGGA [AT]^CCGG[AT]
BsgI 325 GTGCAG GTGCAG(16/14)
BsiEI 1465 CGATCG CG[GA][CT]^CG
BsiHKAI 442 GTGCTC G[AT]GC[AT]^C
BsmFI 690 GTCCC GGGAC(10/14)
BsmI 296 GCATTC GAATGC(1/-1)
Bsp1407I 975 TGTACA T^GTACA
BspLU11I 1432 ACATGT A^CATGT
BspMI 1020 ACCTGC ACCTGC(4/8)
BsrGI 975 TGTACA T^GTACA
BstUI 21 CGCG CG^CG
CfoI 140 GCGC GCG^C
CpoI 183 CGGACCG CG^G[AT]CCG
DraI 1345 TTTAAA TTT^AAA
DraII 1071 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 968 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 63 GACTAGTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 739 GAAGAG CTCTTC(1/4)
Eco47III 139 AGCGCT AGC^GCT
EcoO109I 1071 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRV 796 GATATC GAT^ATC
Fnu4HI 1422 GCAGC GC^[ACGT]GC
HaeII 139 AGCGCT [GA]GCGC^[CT]
HhaI 140 GCGC GCG^C
HinP1I 140 GCGC G^CGC
ItaI 1422 GCAGC GC^[ACGT]GC
Ksp632I 739 GAAGAG CTCTTC(1/4)
MspA1I 556 CAGCTG C[AC]G^C[GT]G
MvnI 21 CGCG CG^CG
NciI 540 CCGGG CC^[GC]GG
PvuI 1465 CGATCG CGAT^CG
PvuII 556 CAGCTG CAG^CTG
RsrII 183 CGGACCG CG^G[AT]CCG
SpeI 64 ACTAGT A^CTAGT
SspBI 975 TGTACA T^GTACA
SspI 524 AATATT AAT^ATT
TseI 1422 GCAGC G^C[AT]GC
XcmI 469 CCAGGCTTCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1113 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 187 CCGC CCGC(-3/-1)
22 GCGG
AfaI 544 GTAC GT^AC
976 GTAC
AlwI 243 GATCC GGATC(4/5)
1056 GGATC
BclI 612 TGATCA T^GATCA
852 TGATCA
BglII 260 AGATCT A^GATCT
1218 AGATCT
BmyI 442 GTGCTC G[AGT]GC[ACT]^C
493 GAGCCC
Bsp1286I 442 GTGCTC G[AGT]GC[ACT]^C
493 GAGCCC
BspHI 171 TCATGA T^CATGA
1235 TCATGA
EcoT14I 305 CCAAGG C^C[AT][AT]GG
1000 CCAAGG
FbaI 612 TGATCA T^GATCA
852 TGATCA
HapII 182 CCGG C^CGG
540 CCGG
HincII 702 GTTGAC GT[CT]^[GA]AC
1184 GTCAAC
HindII 702 GTTGAC GT[CT]^[GA]AC
1184 GTCAAC
HindIII 1116 AAGCTT A^AGCTT
1284 AAGCTT
HpaII 182 CCGG C^CGG
540 CCGG
HphI 961 GGTGA GGTGA(8/7)
1417 GGTGA
MaeIII 412 GTCAC ^GT[ACGT]AC
1193 GTGAC
MspI 182 CCGG C^CGG
540 CCGG
MwoI 381 GCAGAGGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
486 GCCAGCAGAGC
NspI 1432 ACATGT [GA]CATG^[CT]
1510 GCATGT
PflMI 420 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
498 CCAACCAATGG
PleI 771 GACTC GAGTC(4/5)
688 GAGTC
RcaI 171 TCATGA T^CATGA
1235 TCATGA
RsaI 544 GTAC GT^AC
976 GTAC
StyI 305 CCAAGG C^C[AT][AT]GG
1000 CCAAGG
TaqI 990 TCGA T^CGA
1468 TCGA
Tsp45I 412 GTCAC ^GT[GC]AC
1193 GTGAC
TthHB8I 990 TCGA T^CGA
1468 TCGA
Van91I 420 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
498 CCAACCAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcsI AluI AlwNI ApoI AvaII
BfaI BpmI BsaJI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EcoRI EcoRII FokI HaeIII HinfI
MaeI MboI MboII MflI MnlI
MseI MslI MvaI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
SmlI TfiI Tru9I Tsp509I TspRI
XhoII