Restriction map for
pF1KB7570
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BbeI BcgI BciVI
BfrI BglI BglII BlnI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I FseI
FspI HgaI Hin1I HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MslI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SfaNI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AcsI 451 AAATTC [GA]^AATT[CT]
AfaI 182 GTAC GT^AC
AlwI 87 GATCC GGATC(4/5)
ApoI 451 AAATTC [GA]^AATT[CT]
Asp700I 447 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 270 CCCGGG C^[CT]CG[GA]G
AvaII 258 GGTCC G^G[AT]CC
BbrPI 752 CACGTG CAC^GTG
BbsI 531 GAAGAC GAAGAC(2/6)
BclI 224 TGATCA T^GATCA
BfaI 346 CTAG C^TAG
BlpI 24 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 24 GCTGAGC GC^T[ACGT]AGC
BpuAI 531 GAAGAC GAAGAC(2/6)
BsaAI 752 CACGTG [CT]AC^GT[GA]
BsmBI 364 GAGACG CGTCTC(1/5)
BsmI 174 GCATTC GAATGC(1/-1)
BsoBI 270 CCCGGG C^[CT]CG[GA]G
BspHI 74 TCATGA T^CATGA
BsrBI 211 CCGCTC CCGCTC(-3/-3)
BstEII 303 GGTCACC G^GT[ACGT]ACC
BstPI 303 GGTCACC G^GT[ACGT]ACC
BstYI 86 AGATCC [GA]^GATC[CT]
CelII 24 GCTGAGC GC^T[ACGT]AGC
CfoI 60 GCGC GCG^C
EaeI 36 CGGCCA [CT]^GGCC[GA]
EcoNI 90 CCTCTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 303 GGTCACC G^GT[ACGT]ACC
EcoT14I 95 CCAAGG C^C[AT][AT]GG
FbaI 224 TGATCA T^GATCA
HaeII 59 GGCGCT [GA]GCGC^[CT]
HhaI 60 GCGC GCG^C
HinP1I 60 GCGC G^CGC
HincII 474 GTCAAC GT[CT]^[GA]AC
HindII 474 GTCAAC GT[CT]^[GA]AC
MaeI 346 CTAG C^TAG
MaeII 753 ACGT A^CGT
MaeIII 304 GTCAC ^GT[ACGT]AC
MflI 86 AGATCC [GA]^GATC[CT]
PmaCI 752 CACGTG CAC^GTG
PmlI 752 CACGTG CAC^GTG
RcaI 74 TCATGA T^CATGA
RsaI 182 GTAC GT^AC
SmaI 270 CCCGGG CCC^GGG
StyI 95 CCAAGG C^C[AT][AT]GG
TaqI 80 TCGA T^CGA
Tsp45I 304 GTCAC ^GT[GC]AC
Tsp509I 452 AATT ^AATT
TthHB8I 80 TCGA T^CGA
XhoII 86 AGATCC [GA]^GATC[CT]
XmaCI 270 CCCGGG C^CCGGG
XmaI 270 CCCGGG C^CCGGG
XmnI 447 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 283 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
502 CAGTCTCTG
AspHI 27 GAGCTC G[AT]GC[AT]^C
724 GAGCTC
BbvCI 18 GCTGAGG CCTCAGC(-5/-2)
322 GCTGAGG
BpmI 442 CTGGAG CTGGAG(16/14)
700 CTGGAG
BsaI 100 GAGACC GGTCTC(1/5)
315 GGTCTC
BseRI 214 CTCCTC GAGGAG(10/8)
721 GAGGAG
BsiHKAI 27 GAGCTC G[AT]GC[AT]^C
724 GAGCTC
BspMI 103 ACCTGC ACCTGC(4/8)
525 GCAGGT
BstF5I 633 CATCC GGATG(2/0)
554 GGATG
DraII 326 AGGCCCC [GA]G^G[ACGT]CC[CT]
341 AGGCCCT
EcoO109I 326 AGGCCCC [GA]G^G[ACGT]CC[CT]
341 AGGCCCT
FokI 633 CATCC GGATG(9/13)
554 GGATG
MseI 578 TTAA T^TAA
832 TTAA
MspA1I 520 CAGCTG C[AC]G^C[GT]G
697 CAGCTG
NlaIII 75 CATG CATG^
435 CATG
PstI 427 CTGCAG CTGCA^G
523 CTGCAG
PvuII 520 CAGCTG CAG^CTG
697 CAGCTG
SacI 27 GAGCTC GAGCT^C
724 GAGCTC
SfcI 427 CTGCAG C^T[GA][CT]AG
523 CTGCAG
TfiI 275 GAATC G^A[AT]TC
546 GAATC
Tru9I 578 TTAA T^TAA
832 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI BanII BbvI BcnI
BmyI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstNI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
FauI Fnu4HI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MboI
MboII MnlI MspI MvaI MwoI
NciI NdeII NlaIV PleI Sau3AI
Sau96I ScrFI SexAI TseI TspRI