Restriction map for
pF1KB5707
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# NO CUT ENZYMES ( 118 enzymes) #
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Acc65I AccII AclI AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BcgI BglI BlnI BsaBI BsiEI
BsiWI BsoBI BspDI BspLU11I BsrBI
BsrFI BssHII BstBI BstEII BstPI
BstUI Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FseI FspI
HaeII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MscI
MslI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccIII 1455 TCCGGA T^CCGGA
AflII 286 CTTAAG C^TTAAG
AflIII 194 ACGTGT A^C[GA][CT]GT
AlwNI 1840 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 1755 GGACC G^G[AT]CC
BbsI 1285 GAAGAC GAAGAC(2/6)
BciVI 248 GGATAC GTATCC(6/5)
BclI 29 TGATCA T^GATCA
BcnI 1435 CCCGG CC^[GC]GG
BfrI 286 CTTAAG C^TTAAG
BglII 1059 AGATCT A^GATCT
BlpI 326 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 326 GCTCAGC GC^T[ACGT]AGC
BpuAI 1285 GAAGAC GAAGAC(2/6)
BsaAI 1623 CACGTA [CT]AC^GT[GA]
BsaI 785 GGTCTC GGTCTC(1/5)
BsaWI 1455 TCCGGA [AT]^CCGG[AT]
BseAI 1455 TCCGGA T^CCGGA
BsgI 1867 CTGCAC GTGCAG(16/14)
BsmI 2323 GCATTC GAATGC(1/-1)
Bsp1407I 395 TGTACA T^GTACA
BspEI 1455 TCCGGA T^CCGGA
BsrGI 395 TGTACA T^GTACA
BssSI 54 CACGAG CACGAG(-5/-1)
Bst1107I 1377 GTATAC GTA^TAC
BstAPI 2137 GCAGGGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1377 GTATAC GTA^TAC
CelII 326 GCTCAGC GC^T[ACGT]AGC
CfoI 2343 GCGC GCG^C
DraII 1215 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 1930 CGGCCA [CT]^GGCC[GA]
EcoO109I 1215 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 451 GATATC GAT^ATC
EcoT14I 764 CCATGG C^C[AT][AT]GG
FbaI 29 TGATCA T^GATCA
HhaI 2343 GCGC GCG^C
HinP1I 2343 GCGC G^CGC
HincII 1564 GTTGAC GT[CT]^[GA]AC
HindII 1564 GTTGAC GT[CT]^[GA]AC
MroI 1455 TCCGGA T^CCGGA
NciI 1435 CCCGG CC^[GC]GG
NcoI 764 CCATGG C^CATGG
PflMI 1186 CCAGTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 945 GAGCTC GAGCT^C
SexAI 514 ACCTGGT A^CC[AT]GGT
SspBI 395 TGTACA T^GTACA
SspI 2288 AATATT AAT^ATT
StyI 764 CCATGG C^C[AT][AT]GG
Van91I 1186 CCAGTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AatII 422 GACGTC GACGT^C
507 GACGTC
AccI 510 GTCTAC GT^[AC][GT]AC
1377 GTATAC
BpmI 79 CTGGAG CTGGAG(16/14)
1345 CTGGAG
BspHI 1098 TCATGA T^CATGA
2153 TCATGA
BsrDI 2052 CATTGC GCAATG(2/0)
391 GCAATG
BstXI 595 CCAGGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2238 CCAGAAGTTTGG
DsaI 527 CCGTGG C^C[GA][CT]GG
764 CCATGG
FauI 963 CCCGC CCCGC(4/6)
2221 CCCGC
HindIII 1427 AAGCTT A^AGCTT
2093 AAGCTT
HphI 88 GGTGA GGTGA(8/7)
292 GGTGA
PleI 1452 GACTC GAGTC(4/5)
1127 GAGTC
PstI 1837 CTGCAG CTGCA^G
2005 CTGCAG
RcaI 1098 TCATGA T^CATGA
2153 TCATGA
SapI 815 GAAGAGC GCTCTTC(1/4)
2132 GAAGAGC
SfcI 1837 CTGCAG C^T[GA][CT]AG
2005 CTGCAG
Tsp45I 230 GTGAC ^GT[GC]AC
1848 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AspHI BanII BbvCI
BbvI BfaI BmyI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeIII HapII HgaI
Hin1I HinfI HpaII ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII