Restriction map for
pF1KB9987
##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AflIII AgeI AhdI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BamHI BbeI
BbrPI BcgI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
EcoNI EcoRI EcoT22I FbaI FseI
Hin1I HincII HindII HpaI KasI
KpnI KspI MluI MvnI NarI
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I XbaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccI 1612 GTCTAC GT^[AC][GT]AC
AccIII 196 TCCGGA T^CCGGA
AfaI 1488 GTAC GT^AC
Alw44I 767 GTGCAC G^TGCAC
AocI 1377 CCTTAGG CC^T[ACGT]AGG
Aor51HI 1370 AGCGCT AGC^GCT
ApaLI 767 GTGCAC G^TGCAC
AvaI 872 CTCGAG C^[CT]CG[GA]G
AviII 275 TGCGCA TGC^GCA
BanII 395 GAGCCC G[GA]GC[CT]^C
BbsI 301 GAAGAC GAAGAC(2/6)
BglI 1587 GCCCACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 301 GAAGAC GAAGAC(2/6)
BsaBI 1395 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1122 GAGACC GGTCTC(1/5)
BsaWI 196 TCCGGA [AT]^CCGG[AT]
BseAI 196 TCCGGA T^CCGGA
BsmI 38 GCATTC GAATGC(1/-1)
BsoBI 872 CTCGAG C^[CT]CG[GA]G
BspEI 196 TCCGGA T^CCGGA
BsrFI 900 GCCGGC [GA]^CCGG[CT]
BstEII 264 GGTCACC G^GT[ACGT]ACC
BstPI 264 GGTCACC G^GT[ACGT]ACC
BstXI 822 CCATTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 597 GGATCT [GA]^GATC[CT]
Bsu36I 1377 CCTTAGG CC^T[ACGT]AGG
Cfr10I 900 GCCGGC [GA]^CCGG[CT]
Eco47III 1370 AGCGCT AGC^GCT
Eco81I 1377 CCTTAGG CC^T[ACGT]AGG
EcoO65I 264 GGTCACC G^GT[ACGT]ACC
EcoRV 1351 GATATC GAT^ATC
FauI 1006 CCCGC CCCGC(4/6)
FspI 275 TGCGCA TGC^GCA
HgaI 376 GCGTC GACGC(5/10)
HindIII 338 AAGCTT A^AGCTT
MamI 1395 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1316 CAATTG C^AATTG
MflI 597 GGATCT [GA]^GATC[CT]
MroI 196 TCCGGA T^CCGGA
MseI 1358 TTAA T^TAA
MunI 1316 CAATTG C^AATTG
NaeI 900 GCCGGC GCC^GGC
NcoI 805 CCATGG C^CATGG
NdeI 854 CATATG CA^TATG
NgoMIV 900 GCCGGC G^CCGGC
PaeR7I 872 CTCGAG C^TCGAG
PpuMI 701 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 1488 GTAC GT^AC
SapI 1557 GCTCTTC GCTCTTC(1/4)
SexAI 845 ACCAGGT A^CC[AT]GGT
SspI 101 AATATT AAT^ATT
Tru9I 1358 TTAA T^TAA
Tsp45I 265 GTCAC ^GT[GC]AC
XhoI 872 CTCGAG C^TCGAG
XhoII 597 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 1276 AAATTC [GA]^AATT[CT]
1536 GAATTT
AlwI 894 GATCC GGATC(4/5)
597 GGATC
ApoI 1276 AAATTC [GA]^AATT[CT]
1536 GAATTT
BalI 483 TGGCCA TGG^CCA
858 TGGCCA
BbvCI 7 CCTCAGC CCTCAGC(-5/-2)
524 CCTCAGC
BciVI 253 GTATCC GTATCC(6/5)
756 GTATCC
BseRI 5 CTCCTC GAGGAG(10/8)
1679 CTCCTC
BsrI 481 ACTGG ACTGG(1/-1)
932 CCAGT
BstAPI 915 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
948 GCACCCTGTGC
DraIII 844 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
949 CACCCTGTG
DsaI 805 CCATGG C^C[GA][CT]GG
1684 CCACGG
EaeI 483 TGGCCA [CT]^GGCC[GA]
858 TGGCCA
HaeII 1370 AGCGCT [GA]GCGC^[CT]
1441 GGCGCT
MaeII 435 ACGT A^CGT
1433 ACGT
MaeIII 265 GTCAC ^GT[ACGT]AC
692 GTTAC
MluNI 483 TGGCCA TGG^CCA
858 TGGCCA
MscI 483 TGGCCA TGG^CCA
858 TGGCCA
PflMI 549 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
980 CCAGCCCCTGG
Van91I 549 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
980 CCAGCCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AluI AlwNI AspHI AvaII
BanI BbvI BcnI BfaI BmyI
BpmI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MboI MboII MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI TseI Tsp509I TspRI TthHB8I
XcmI