Restriction map for
pF1KB5941
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BbsI BbvCI BclI
BfrI BlnI BpuAI BsaI BsaWI
BseAI BseRI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eam1105I Eco81I EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MfeI MluI MroI MseI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp509I Tth111I XbaI
XcmI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AflIII 338 ACACGT A^C[GA][CT]GT
AlwI 591 GATCC GGATC(4/5)
Asp700I 142 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1184 TGGCCA TGG^CCA
BanI 72 GGCGCC G^G[CT][GA]CC
BbeI 72 GGCGCC GGCGC^C
BcgI 344 CGAGCTTCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1095 GGATAC GTATCC(6/5)
BglII 842 AGATCT A^GATCT
BsaAI 481 CACGTA [CT]AC^GT[GA]
BsaBI 1096 GATACCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 554 CGGCCG CG[GA][CT]^CG
BsrFI 556 GCCGGC [GA]^CCGG[CT]
BssHII 662 GCGCGC G^CGCGC
Cfr10I 556 GCCGGC [GA]^CCGG[CT]
EagI 554 CGGCCG C^GGCCG
EclXI 554 CGGCCG C^GGCCG
Eco52I 554 CGGCCG C^GGCCG
EcoNI 276 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 395 GATATC GAT^ATC
KasI 72 GGCGCC G^GCGCC
MamI 1096 GATACCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1184 TGGCCA TGG^CCA
MscI 1184 TGGCCA TGG^CCA
NaeI 556 GCCGGC GCC^GGC
NarI 72 GGCGCC GG^CGCC
NgoMIV 556 GCCGGC G^CCGGC
NspI 1086 GCATGC [GA]CATG^[CT]
PaeR7I 247 CTCGAG C^TCGAG
PflMI 1156 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 913 CAGCTG CAG^CTG
SapI 142 GAAGAGC GCTCTTC(1/4)
SfoI 72 GGCGCC GGC^GCC
SmlI 247 CTCGAG C^T[CT][GA]AG
SphI 1086 GCATGC GCATG^C
StuI 882 AGGCCT AGG^CCT
Tsp45I 370 GTCAC ^GT[GC]AC
Van91I 1156 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 247 CTCGAG C^TCGAG
XmnI 142 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 522 GACGTC GACGT^C
547 GACGTC
AlwNI 1157 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1484 CAGCCGCTG
Aor51HI 205 AGCGCT AGC^GCT
1007 AGCGCT
AspHI 427 GTGCTC G[AT]GC[AT]^C
802 GTGCTC
BglI 64 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
118 GCCTTGCTGGC
BsgI 277 CTGCAC GTGCAG(16/14)
957 CTGCAC
BsiHKAI 427 GTGCTC G[AT]GC[AT]^C
802 GTGCTC
BsmBI 524 CGTCTC CGTCTC(1/5)
549 CGTCTC
BspMI 626 ACCTGC ACCTGC(4/8)
981 ACCTGC
BsrBI 163 GAGCGG CCGCTC(-3/-3)
474 GAGCGG
BsrI 1072 CCAGT ACTGG(1/-1)
1142 CCAGT
BssSI 296 CTCGTG CACGAG(-5/-1)
775 CTCGTG
BstXI 927 CCAGAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1505 CCAGCTATGTGG
BstYI 590 AGATCC [GA]^GATC[CT]
842 AGATCT
DpnII 591 GATC ^GATC
843 GATC
DraIII 481 CACGTAGTG CAC[ACGT][ACGT][ACGT]^GTG
606 CACCGAGTG
EarI 886 CTCTTC CTCTTC(1/4)
142 GAAGAG
Eco47III 205 AGCGCT AGC^GCT
1007 AGCGCT
HinfI 1381 GACTC G^A[ACGT]TC
1414 GACTC
Ksp632I 886 CTCTTC CTCTTC(1/4)
142 GAAGAG
MaeIII 370 GTCAC ^GT[ACGT]AC
905 GTAAC
MboI 591 GATC ^GATC
843 GATC
MflI 590 AGATCC [GA]^GATC[CT]
842 AGATCT
MspA1I 913 CAGCTG C[AC]G^C[GT]G
1487 CCGCTG
NdeII 591 GATC ^GATC
843 GATC
PleI 1381 GACTC GAGTC(4/5)
1414 GACTC
PpuMI 974 AGGACCC [GA]G^G[AT]CC[CT]
1148 AGGACCT
Sau3AI 591 GATC ^GATC
843 GATC
SmaI 131 CCCGGG CCC^GGG
219 CCCGGG
XhoII 590 AGATCC [GA]^GATC[CT]
842 AGATCT
XmaCI 131 CCCGGG C^CCGGG
219 CCCGGG
XmaI 131 CCCGGG C^CCGGG
219 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
ApaI AvaI AvaII BanII BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BsaHI BsaJI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BssKI
BstF5I BstNI BstUI Cac8I CelII
CfoI Cfr13I DdeI DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI MaeI MaeII MboII
MnlI MslI MspI MvaI MvnI
MwoI NciI NcoI NlaIII NlaIV
RsaI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI TspRI TthHB8I