Restriction map for
pF1KB5923
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AclI AflIII AgeI AhdI
AlwNI Aor51HI AscI AseI AsnI
AspEI AspI AviII BamHI BbrPI
BbvCI BciVI BclI BglII BsaBI
BsgI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BssHII BstAPI
BstBI ClaI CpoI DraI Eam1105I
EarI Eco47III EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI Ksp632I KspI MamI MfeI
MluI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 65 enzymes) #
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Acc65I 1411 GGTACC G^GTACC
AccI 1371 GTATAC GT^[AC][GT]AC
AccIII 854 TCCGGA T^CCGGA
AcsI 958 GAATTC [GA]^AATT[CT]
AflII 1162 CTTAAG C^TTAAG
Alw44I 782 GTGCAC G^TGCAC
AocI 1213 CCTGAGG CC^T[ACGT]AGG
ApaI 1403 GGGCCC GGGCC^C
ApaLI 782 GTGCAC G^TGCAC
ApoI 958 GAATTC [GA]^AATT[CT]
Asp718I 1411 GGTACC G^GTACC
AvrII 418 CCTAGG C^CTAGG
BbeI 99 GGCGCC GGCGC^C
BcgI 209 CGACCTTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1162 CTTAAG C^TTAAG
BglI 91 GCCGCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 418 CCTAGG C^CTAGG
BlpI 222 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 222 GCTGAGC GC^T[ACGT]AGC
BsaAI 817 TACGTG [CT]AC^GT[GA]
BsaWI 854 TCCGGA [AT]^CCGG[AT]
BseAI 854 TCCGGA T^CCGGA
BseRI 399 GAGGAG GAGGAG(10/8)
BsiEI 20 CGGCCG CG[GA][CT]^CG
BsmBI 1435 CGTCTC CGTCTC(1/5)
BsmI 711 GAATGC GAATGC(1/-1)
BspEI 854 TCCGGA T^CCGGA
BsrFI 1039 ACCGGC [GA]^CCGG[CT]
BssSI 682 CACGAG CACGAG(-5/-1)
Bst1107I 1371 GTATAC GTA^TAC
BstEII 1134 GGTGACC G^GT[ACGT]ACC
BstPI 1134 GGTGACC G^GT[ACGT]ACC
BstXI 995 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1071 GGATCT [GA]^GATC[CT]
BstZ17I 1371 GTATAC GTA^TAC
Bsu36I 1213 CCTGAGG CC^T[ACGT]AGG
CelII 222 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1039 ACCGGC [GA]^CCGG[CT]
DrdI 1468 GACATCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 20 CGGCCG C^GGCCG
EclXI 20 CGGCCG C^GGCCG
Eco52I 20 CGGCCG C^GGCCG
Eco81I 1213 CCTGAGG CC^T[ACGT]AGG
EcoNI 427 CCTAGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1134 GGTGACC G^GT[ACGT]ACC
EcoRI 958 GAATTC G^AATTC
KasI 99 GGCGCC G^GCGCC
KpnI 1411 GGTACC GGTAC^C
MaeII 818 ACGT A^CGT
MflI 1071 GGATCT [GA]^GATC[CT]
MroI 854 TCCGGA T^CCGGA
MseI 1163 TTAA T^TAA
NarI 99 GGCGCC GG^CGCC
NspI 477 GCATGC [GA]CATG^[CT]
PaeR7I 188 CTCGAG C^TCGAG
SacI 39 GAGCTC GAGCT^C
SfaNI 519 GCATC GCATC(5/9)
SfoI 99 GGCGCC GGC^GCC
SphI 477 GCATGC GCATG^C
TfiI 616 GATTC G^A[AT]TC
Tru9I 1163 TTAA T^TAA
Tsp509I 959 AATT ^AATT
XcmI 1442 CCAACAACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 188 CTCGAG C^TCGAG
XhoII 1071 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 625 GATCC GGATC(4/5)
1071 GGATC
Asp700I 711 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
865 GAAATCATTC
BanI 99 GGCGCC G^G[CT][GA]CC
1411 GGTACC
BsaI 1151 GAGACC GGTCTC(1/5)
1421 GAGACC
BsrI 458 ACTGG ACTGG(1/-1)
786 ACTGG
DraIII 31 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
760 CACCAAGTG
DsaI 23 CCGTGG C^C[GA][CT]GG
1425 CCGTGG
EcoT14I 418 CCTAGG C^C[AT][AT]GG
995 CCAAGG
MslI 870 CATTCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1128 CATTGGGGTG
MspA1I 220 CAGCTG C[AC]G^C[GT]G
919 CAGCTG
PleI 443 GACTC GAGTC(4/5)
345 GAGTC
PstI 217 CTGCAG CTGCA^G
1170 CTGCAG
PvuII 220 CAGCTG CAG^CTG
919 CAGCTG
SexAI 32 ACCTGGT A^CC[AT]GGT
548 ACCTGGT
SfcI 217 CTGCAG C^T[GA][CT]AG
1170 CTGCAG
StyI 418 CCTAGG C^C[AT][AT]GG
995 CCAAGG
TaqI 189 TCGA T^CGA
1061 TCGA
Tsp45I 1135 GTGAC ^GT[GC]AC
1297 GTCAC
TthHB8I 189 TCGA T^CGA
1061 TCGA
XmnI 711 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
865 GAAATCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BalI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MluNI
MnlI MscI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PpuMI RsaI Sau3AI Sau96I
ScrFI SmaI SmlI TseI TspRI
Van91I XmaCI XmaI