Restriction map for
pF1KB5911
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflIII AgeI AhdI Alw44I AlwI
AocI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BamHI BbrPI BbvCI BclI BfaI
BglII BlnI BsaBI BsaI BseAI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI Bst1107I BstBI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII Eam1105I Eco81I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HinfI HpaI KpnI MaeI
MamI MfeI MflI MluI MroI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI TaqI TfiI Tsp509I
Tth111I TthHB8I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 58 GTCTAC GT^[AC][GT]AC
AflII 532 CTTAAG C^TTAAG
Aor51HI 1030 AGCGCT AGC^GCT
Asp700I 231 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 15 GAAGAC GAAGAC(2/6)
BcgI 703 CGAGAGATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 721 GGATAC GTATCC(6/5)
BfrI 532 CTTAAG C^TTAAG
BlpI 1461 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1461 GCTGAGC GC^T[ACGT]AGC
BpuAI 15 GAAGAC GAAGAC(2/6)
BsaAI 490 TACGTG [CT]AC^GT[GA]
BsaWI 1088 TCCGGT [AT]^CCGG[AT]
BseRI 1309 GAGGAG GAGGAG(10/8)
BsiEI 140 CGGCCG CG[GA][CT]^CG
BsrFI 767 ACCGGC [GA]^CCGG[CT]
BssSI 607 CACGAG CACGAG(-5/-1)
BstAPI 1112 GCAGCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 158 CCACGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1461 GCTGAGC GC^T[ACGT]AGC
Cfr10I 767 ACCGGC [GA]^CCGG[CT]
DdeI 1462 CTGAG C^T[ACGT]AG
DrdI 354 GACCCGCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 140 CGGCCG C^GGCCG
EclXI 140 CGGCCG C^GGCCG
Eco47III 1030 AGCGCT AGC^GCT
Eco52I 140 CGGCCG C^GGCCG
KspI 1412 CCGCGG CCGC^GG
MseI 533 TTAA T^TAA
PpuMI 299 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 574 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 409 GAGCTC GAGCT^C
SacII 1412 CCGCGG CCGC^GG
SmlI 532 CTTAAG C^T[CT][GA]AG
StuI 929 AGGCCT AGG^CCT
Tru9I 533 TTAA T^TAA
XmnI 231 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 450 GTAC GT^AC
1125 GTAC
ApaI 243 GGGCCC GGGCC^C
1499 GGGCCC
BbeI 133 GGCGCC GGCGC^C
335 GGCGCC
BsgI 250 CTGCAC GTGCAG(16/14)
1344 CTGCAC
BsmFI 1289 GGGAC GGGAC(10/14)
552 GTCCC
BssHII 1137 GCGCGC G^CGCGC
1383 GCGCGC
BstEII 168 GGTCACC G^GT[ACGT]ACC
618 GGTCACC
BstPI 168 GGTCACC G^GT[ACGT]ACC
618 GGTCACC
EarI 676 CTCTTC CTCTTC(1/4)
1339 CTCTTC
EcoO65I 168 GGTCACC G^GT[ACGT]ACC
618 GGTCACC
EcoT14I 401 CCAAGG C^C[AT][AT]GG
668 CCAAGG
KasI 133 GGCGCC G^GCGCC
335 GGCGCC
Ksp632I 676 CTCTTC CTCTTC(1/4)
1339 CTCTTC
NarI 133 GGCGCC GG^CGCC
335 GGCGCC
PflMI 828 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1122 CCAGTACCTGG
PstI 429 CTGCAG CTGCA^G
745 CTGCAG
RsaI 450 GTAC GT^AC
1125 GTAC
SexAI 266 ACCTGGT A^CC[AT]GGT
1127 ACCTGGT
SfoI 133 GGCGCC GGC^GCC
335 GGCGCC
SphI 324 GCATGC GCATG^C
1379 GCATGC
StyI 401 CCAAGG C^C[AT][AT]GG
668 CCAAGG
Van91I 828 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1122 CCAGTACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AspHI AvaI AvaII AviII BalI
BanI BanII BbvI BcnI BglI
BmyI BpmI BsaHI BsaJI BsiHKAI
BsiYI BslI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DpnII DraII
DsaI EaeI EcoNI EcoO109I EcoRII
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI TseI Tsp45I
TspRI XcmI