Restriction map for
pF1KB5868
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BanI
BanII BbeI BbrPI BclI BfaI
BfrI BglI BglII BlnI BsaBI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Cfr10I ClaI CpoI
DraI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoT14I EcoT22I FauI FbaI
FseI FspI Hin1I HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MluI MroI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI TfiI TthHB8I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcsI 943 AAATTC [GA]^AATT[CT]
Alw44I 528 GTGCAC G^TGCAC
AlwNI 377 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 281 CCTCAGG CC^T[ACGT]AGG
ApaLI 528 GTGCAC G^TGCAC
ApoI 943 AAATTC [GA]^AATT[CT]
AspI 145 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 340 CCCGGG C^[CT]CG[GA]G
BalI 356 TGGCCA TGG^CCA
BbsI 877 GAAGAC GAAGAC(2/6)
BpmI 1393 CTGGAG CTGGAG(16/14)
BpuAI 877 GAAGAC GAAGAC(2/6)
BsaAI 72 CACGTA [CT]AC^GT[GA]
BsaI 1015 GAGACC GGTCTC(1/5)
BsoBI 340 CCCGGG C^[CT]CG[GA]G
BspLU11I 991 ACATGT A^CATGT
BspMI 751 ACCTGC ACCTGC(4/8)
BssSI 1351 CTCGTG CACGAG(-5/-1)
BstXI 311 CCAGCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 281 CCTCAGG CC^T[ACGT]AGG
DraIII 502 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1319 GACAAACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 281 CCTCAGG CC^T[ACGT]AGG
EcoRV 611 GATATC GAT^ATC
HaeII 36 GGCGCT [GA]GCGC^[CT]
HincII 184 GTCAAC GT[CT]^[GA]AC
HindII 184 GTCAAC GT[CT]^[GA]AC
HinfI 30 GACTC G^A[ACGT]TC
HphI 978 GGTGA GGTGA(8/7)
MfeI 730 CAATTG C^AATTG
MluNI 356 TGGCCA TGG^CCA
MscI 356 TGGCCA TGG^CCA
MspA1I 428 CAGCTG C[AC]G^C[GT]G
MunI 730 CAATTG C^AATTG
PflFI 145 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 30 GACTC GAGTC(4/5)
PpuMI 1420 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 428 CAGCTG CAG^CTG
SmaI 340 CCCGGG CCC^GGG
SmlI 900 CTCAAG C^T[CT][GA]AG
Tth111I 145 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 711 CCAGTGCAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 340 CCCGGG C^CCGGG
XmaI 340 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 637 GTAGAC GT^[AC][GT]AC
1180 GTCTAC
AfaI 75 GTAC GT^AC
653 GTAC
AflIII 506 ACGTGT A^C[GA][CT]GT
991 ACATGT
BamHI 105 GGATCC G^GATCC
583 GGATCC
BbvCI 250 CCTCAGC CCTCAGC(-5/-2)
436 CCTCAGC
BcgI 525 CGAGTGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1126 CGATGTGTTTGC
BlpI 793 GCTCAGC GC^T[ACGT]AGC
852 GCTAAGC
Bpu1102I 793 GCTCAGC GC^T[ACGT]AGC
852 GCTAAGC
BsgI 476 GTGCAG GTGCAG(16/14)
714 GTGCAG
CelII 793 GCTCAGC GC^T[ACGT]AGC
852 GCTAAGC
CfoI 37 GCGC GCG^C
441 GCGC
DraII 378 AGGCCCT [GA]G^G[ACGT]CC[CT]
1420 GGGACCT
EaeI 356 TGGCCA [CT]^GGCC[GA]
396 TGGCCG
EcoO109I 378 AGGCCCT [GA]G^G[ACGT]CC[CT]
1420 GGGACCT
HgaI 157 GACGC GACGC(5/10)
540 GACGC
HhaI 37 GCGC GCG^C
441 GCGC
HinP1I 37 GCGC G^CGC
441 GCGC
MaeII 73 ACGT A^CGT
506 ACGT
MseI 1283 TTAA T^TAA
1444 TTAA
MslI 593 CATGTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
950 CATGTATGTG
NspI 592 GCATGT [GA]CATG^[CT]
991 ACATGT
PstI 368 CTGCAG CTGCA^G
533 CTGCAG
RsaI 75 GTAC GT^AC
653 GTAC
Tru9I 1283 TTAA T^TAA
1444 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BbvI BciVI BcnI BmyI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HapII HpaII ItaI MaeIII
MboI MboII MflI MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
SfcI TseI Tsp45I Tsp509I TspRI
XhoII