Restriction map for
pF1KB5841
##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AlwI ApoI
AseI AsnI Asp718I AspI AviII
AvrII BamHI BbsI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsiWI
BsmBI BsmI Bsp1407I BspHI BspLU11I
BsrDI BsrGI Bst1107I BstAPI BstBI
BstXI BstYI BstZ17I CelII CpoI
DraI EagI EclXI Eco52I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI HpaI KpnI KspI MamI
MfeI MflI MluI MunI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SspI SwaI
Tsp509I Tth111I XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccIII 272 TCCGGA T^CCGGA
AfaI 636 GTAC GT^AC
AhdI 553 GACGGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 437 GTGCAC G^TGCAC
AlwNI 827 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 500 AGCGCT AGC^GCT
ApaLI 437 GTGCAC G^TGCAC
AscI 429 GGCGCGCC GG^CGCGCC
Asp700I 327 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 553 GACGGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 508 CCCGAG C^[CT]CG[GA]G
BglI 1144 GCCGGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 272 TCCGGA [AT]^CCGG[AT]
BseAI 272 TCCGGA T^CCGGA
BseRI 424 GAGGAG GAGGAG(10/8)
BsiEI 365 CGACCG CG[GA][CT]^CG
BsoBI 508 CCCGAG C^[CT]CG[GA]G
BspDI 643 ATCGAT AT^CGAT
BspEI 272 TCCGGA T^CCGGA
BsrBI 815 CCGCTC CCGCTC(-3/-3)
BsrFI 473 GCCGGC [GA]^CCGG[CT]
BssHII 430 GCGCGC G^CGCGC
BssSI 453 CTCGTG CACGAG(-5/-1)
BstEII 564 GGTCACC G^GT[ACGT]ACC
BstF5I 1002 CATCC GGATG(2/0)
BstPI 564 GGTCACC G^GT[ACGT]ACC
Cfr10I 473 GCCGGC [GA]^CCGG[CT]
ClaI 643 ATCGAT AT^CGAT
DraIII 20 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 679 GACGCCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 683 CCGTGG C^C[GA][CT]GG
Eam1105I 553 GACGGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 500 AGCGCT AGC^GCT
EcoO65I 564 GGTCACC G^GT[ACGT]ACC
EcoT14I 305 CCTTGG C^C[AT][AT]GG
FokI 1002 CATCC GGATG(9/13)
HindIII 1039 AAGCTT A^AGCTT
MroI 272 TCCGGA T^CCGGA
NaeI 473 GCCGGC GCC^GGC
NgoMIV 473 GCCGGC G^CCGGC
PpuMI 828 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 127 CTGCAG CTGCA^G
PvuII 953 CAGCTG CAG^CTG
RsaI 636 GTAC GT^AC
SacI 352 GAGCTC GAGCT^C
SbfI 126 CCTGCAGG CCTGCA^GG
SfaNI 579 GATGC GCATC(5/9)
Sse8387I 126 CCTGCAGG CCTGCA^GG
StuI 123 AGGCCT AGG^CCT
StyI 305 CCTTGG C^C[AT][AT]GG
TaqI 644 TCGA T^CGA
TthHB8I 644 TCGA T^CGA
XbaI 75 TCTAGA T^CTAGA
XmnI 327 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 431 CGCG CG^CG
572 CGCG
AocI 12 CCTGAGG CC^T[ACGT]AGG
37 CCTAAGG
BalI 416 TGGCCA TGG^CCA
994 TGGCCA
BbeI 547 GGCGCC GGCGC^C
807 GGCGCC
BsaI 301 GAGACC GGTCTC(1/5)
133 GGTCTC
BsgI 833 CTGCAC GTGCAG(16/14)
840 CTGCAC
BspMI 521 ACCTGC ACCTGC(4/8)
867 ACCTGC
BstUI 431 CGCG CG^CG
572 CGCG
Bsu36I 12 CCTGAGG CC^T[ACGT]AGG
37 CCTAAGG
DdeI 13 CTGAG C^T[ACGT]AG
38 CTAAG
DpnII 285 GATC ^GATC
642 GATC
EaeI 416 TGGCCA [CT]^GGCC[GA]
994 TGGCCA
Eco81I 12 CCTGAGG CC^T[ACGT]AGG
37 CCTAAGG
HincII 688 GTCAAC GT[CT]^[GA]AC
958 GTTGAC
HindII 688 GTCAAC GT[CT]^[GA]AC
958 GTTGAC
KasI 547 GGCGCC G^GCGCC
807 GGCGCC
MboI 285 GATC ^GATC
642 GATC
MluNI 416 TGGCCA TGG^CCA
994 TGGCCA
MscI 416 TGGCCA TGG^CCA
994 TGGCCA
MseI 340 TTAA T^TAA
1085 TTAA
MslI 58 CACTACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
449 CACCCTCGTG
MvnI 431 CGCG CG^CG
572 CGCG
NarI 547 GGCGCC GG^CGCC
807 GGCGCC
NdeII 285 GATC ^GATC
642 GATC
NlaIII 399 CATG CATG^
1158 CATG
Sau3AI 285 GATC ^GATC
642 GATC
SfcI 127 CTGCAG C^T[GA][CT]AG
1171 CTACAG
SfoI 547 GGCGCC GGC^GCC
807 GGCGCC
Tru9I 340 TTAA T^TAA
1085 TTAA
XcmI 710 CCAAAAAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1139 CCACAGCCGGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcyI AluI ApaI AspHI
AvaII BanI BanII BbrPI BbvI
BcnI BfaI BmyI BpmI BsaAI
BsaHI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstNI Cac8I CfoI Cfr13I DraII
EarI EcoO109I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboII MnlI MspA1I MspI MvaI
MwoI NciI NlaIV PflMI PmaCI
PmlI Sau96I ScrFI TfiI TseI
Tsp45I TspRI Van91I