Restriction map for
pF1KB5840
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BpmI BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspEI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstUI BstXI BstZ17I Bsu36I
Cfr10I CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRV FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MseI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SspBI SspI StuI SwaI
Tru9I Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcsI 456 AAATTC [GA]^AATT[CT]
AflIII 710 ACATGT A^C[GA][CT]GT
ApaI 1235 GGGCCC GGGCC^C
ApoI 456 AAATTC [GA]^AATT[CT]
Asp700I 1248 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 802 GAGCTC G[AT]GC[AT]^C
AvaII 415 GGACC G^G[AT]CC
BamHI 899 GGATCC G^GATCC
BlpI 958 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 958 GCTAAGC GC^T[ACGT]AGC
BsaI 302 GAGACC GGTCTC(1/5)
BsiHKAI 802 GAGCTC G[AT]GC[AT]^C
BspDI 405 ATCGAT AT^CGAT
BspHI 1410 TCATGA T^CATGA
BspLU11I 710 ACATGT A^CATGT
BsrBI 50 GAGCGG CCGCTC(-3/-3)
BsrDI 1090 GCAATG GCAATG(2/0)
BstAPI 723 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 174 GGTCACC G^GT[ACGT]ACC
BstPI 174 GGTCACC G^GT[ACGT]ACC
CelII 958 GCTAAGC GC^T[ACGT]AGC
ClaI 405 ATCGAT AT^CGAT
EcoO65I 174 GGTCACC G^GT[ACGT]ACC
EcoT14I 587 CCAAGG C^C[AT][AT]GG
EcoT22I 193 ATGCAT ATGCA^T
FauI 121 GCGGG CCCGC(4/6)
HaeII 887 AGCGCC [GA]GCGC^[CT]
MaeII 1128 ACGT A^CGT
MslI 852 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 193 ATGCAT ATGCA^T
PshAI 217 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 634 CAGCTG CAG^CTG
RcaI 1410 TCATGA T^CATGA
SacI 802 GAGCTC GAGCT^C
ScaI 1034 AGTACT AGT^ACT
SphI 1167 GCATGC GCATG^C
StyI 587 CCAAGG C^C[AT][AT]GG
TaqI 406 TCGA T^CGA
TthHB8I 406 TCGA T^CGA
XcmI 1027 CCAAGTGAGTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1248 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwNI 724 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1382 CAGACTCTG
BcnI 19 CCCGG CC^[GC]GG
1233 CCGGG
BstNI 114 CCTGG CC^[AT]GG
520 CCTGG
DsaI 617 CCGTGG C^C[GA][CT]GG
693 CCACGG
EaeI 537 TGGCCG [CT]^GGCC[GA]
1182 TGGCCG
EarI 1054 GAAGAG CTCTTC(1/4)
1309 GAAGAG
EcoRII 114 CCTGG ^CC[AT]GG
520 CCTGG
Ksp632I 1054 GAAGAG CTCTTC(1/4)
1309 GAAGAG
MvaI 114 CCTGG CC^[AT]GG
520 CCTGG
NciI 19 CCCGG CC^[GC]GG
1233 CCGGG
NspI 710 ACATGT [GA]CATG^[CT]
1167 GCATGC
PleI 1384 GACTC GAGTC(4/5)
448 GAGTC
SbfI 179 CCTGCAGG CCTGCA^GG
570 CCTGCAGG
SfaNI 742 GATGC GCATC(5/9)
732 GCATC
SmlI 393 CTCAAG C^T[CT][GA]AG
931 CTCAAG
Sse8387I 179 CCTGCAGG CCTGCA^GG
570 CCTGCAGG
TfiI 595 GAATC G^A[AT]TC
700 GATTC
Tsp509I 457 AATT ^AATT
965 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI AlwI BanII
BbvI BmyI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrI BssKI BstF5I
BstYI Cac8I CfoI Cfr13I DdeI
DpnII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MflI
MnlI MspA1I MspI MwoI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I ScrFI SfcI TseI Tsp45I
TspRI XhoII