Restriction map for
pF1KB5833
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# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AcyI AfaI AflII AflIII
AgeI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspHI AspI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsgI BsiHKAI
BsiWI BsmI Bsp1286I Bsp1407I BspDI
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII EagI EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoT14I
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HinfI
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MluNI MscI
MseI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tsp509I Tth111I Van91I XcmI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 225 TCCGGA T^CCGGA
AhdI 343 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 300 GATCC GGATC(4/5)
AspEI 343 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BfaI 38 CTAG C^TAG
BglII 417 AGATCT A^GATCT
BsaI 202 GAGACC GGTCTC(1/5)
BsaWI 225 TCCGGA [AT]^CCGG[AT]
BseAI 225 TCCGGA T^CCGGA
BsiEI 167 CGACCG CG[GA][CT]^CG
BsiYI 113 CCGGAAGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 113 CCGGAAGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 319 GAGACG CGTCTC(1/5)
BsmFI 351 GTCCC GGGAC(10/14)
BspEI 225 TCCGGA T^CCGGA
BstEII 273 GGTCACC G^GT[ACGT]ACC
BstF5I 177 GGATG GGATG(2/0)
BstNI 67 CCAGG CC^[AT]GG
BstPI 273 GGTCACC G^GT[ACGT]ACC
CfoI 505 GCGC GCG^C
DraII 349 AGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 321 GACGGCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 235 CCACGG C^C[GA][CT]GG
EaeI 323 CGGCCA [CT]^GGCC[GA]
Eam1105I 343 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 349 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 273 GGTCACC G^GT[ACGT]ACC
EcoRII 67 CCAGG ^CC[AT]GG
EcoRV 447 GATATC GAT^ATC
FokI 177 GGATG GGATG(9/13)
HaeII 504 GGCGCT [GA]GCGC^[CT]
HhaI 505 GCGC GCG^C
HinP1I 505 GCGC G^CGC
MaeI 38 CTAG C^TAG
MaeII 494 ACGT A^CGT
MboII 415 GAAGA GAAGA(8/7)
MroI 225 TCCGGA T^CCGGA
MslI 327 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 249 CAGCGG C[AC]G^C[GT]G
MvaI 67 CCAGG CC^[AT]GG
NlaIV 350 GGTCCC GG[ACGT]^[ACGT]CC
PaeR7I 452 CTCGAG C^TCGAG
PpuMI 349 AGGTCCC [GA]G^G[AT]CC[CT]
SmlI 452 CTCGAG C^T[CT][GA]AG
TaqI 453 TCGA T^CGA
TspRI 207 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 453 TCGA T^CGA
XbaI 37 TCTAGA T^CTAGA
XhoI 452 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AvaII 211 GGACC G^G[AT]CC
350 GGTCC
BseRI 83 CTCCTC GAGGAG(10/8)
289 GAGGAG
BstYI 299 AGATCC [GA]^GATC[CT]
417 AGATCT
DdeI 200 CTGAG C^T[ACGT]AG
437 CTGAG
DpnII 300 GATC ^GATC
418 GATC
HphI 151 GGTGA GGTGA(8/7)
275 TCACC
MaeIII 274 GTCAC ^GT[ACGT]AC
400 GTCAC
MboI 300 GATC ^GATC
418 GATC
MflI 299 AGATCC [GA]^GATC[CT]
417 AGATCT
NdeII 300 GATC ^GATC
418 GATC
Sau3AI 300 GATC ^GATC
418 GATC
SmaI 89 CCCGGG CCC^GGG
353 CCCGGG
Tsp45I 274 GTCAC ^GT[GC]AC
400 GTCAC
XhoII 299 AGATCC [GA]^GATC[CT]
417 AGATCT
XmaCI 89 CCCGGG C^CCGGG
353 CCCGGG
XmaI 89 CCCGGG C^CCGGG
353 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AluI AvaI BbvI BcnI
BpmI BsaJI BsmAI BsoBI BsrBI
BsrI BssKI Cac8I Cfr13I FauI
Fnu4HI HaeIII HapII HpaII ItaI
MnlI MspI MwoI NciI NlaIII
Sau96I ScrFI TseI