Restriction map for
pF1KB9981
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BbrPI BbsI BcgI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BseAI
BsiEI BsiWI BsmAI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
Ksp632I KspI MamI MboII MfeI
MluI MroI MseI MunI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SapI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI SspBI SspI SwaI TfiI
Tru9I Tsp45I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccII 1400 CGCG CG^CG
AcyI 1242 GGCGCC G[GA]^CG[CT]C
ApaI 637 GGGCCC GGGCC^C
AspHI 1502 GAGCAC G[AT]GC[AT]^C
AspI 1144 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1242 GGCGCC GGCGC^C
BbvCI 890 CCTCAGC CCTCAGC(-5/-2)
BsaHI 1242 GGCGCC G[GA]^CG[CT]C
BsaWI 1288 ACCGGA [AT]^CCGG[AT]
BseRI 1135 GAGGAG GAGGAG(10/8)
BsiHKAI 1502 GAGCAC G[AT]GC[AT]^C
BsmI 1121 GCATTC GAATGC(1/-1)
BstUI 1400 CGCG CG^CG
BstXI 1550 CCACTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 1358 CCGTGG C^C[GA][CT]GG
EcoNI 970 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 1242 GGCGCC G[GA]^CG[CT]C
HincII 15 GTCGAC GT[CT]^[GA]AC
HindII 15 GTCGAC GT[CT]^[GA]AC
HinfI 55 GAGTC G^A[ACGT]TC
KasI 1242 GGCGCC G^GCGCC
MaeII 13 ACGT A^CGT
MaeIII 1624 GTAAC ^GT[ACGT]AC
MvnI 1400 CGCG CG^CG
NarI 1242 GGCGCC GG^CGCC
PflFI 1144 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 55 GAGTC GAGTC(4/5)
SalI 15 GTCGAC G^TCGAC
SbfI 1347 CCTGCAGG CCTGCA^GG
SfoI 1242 GGCGCC GGC^GCC
Sse8387I 1347 CCTGCAGG CCTGCA^GG
StuI 1199 AGGCCT AGG^CCT
Tth111I 1144 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccI 15 GTCGAC GT^[AC][GT]AC
439 GTCTAC
AocI 335 CCTCAGG CC^T[ACGT]AGG
937 CCTGAGG
BalI 2 TGGCCA TGG^CCA
851 TGGCCA
BamHI 1062 GGATCC G^GATCC
1212 GGATCC
BanII 637 GGGCCC G[GA]GC[CT]^C
799 GAGCCC
BglII 606 AGATCT A^GATCT
1293 AGATCT
BsgI 1203 CTGCAC GTGCAG(16/14)
1615 GTGCAG
BspMI 588 ACCTGC ACCTGC(4/8)
1519 GCAGGT
BstAPI 1483 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1641 GCAGGCCCTGC
Bsu36I 335 CCTCAGG CC^T[ACGT]AGG
937 CCTGAGG
Eco81I 335 CCTCAGG CC^T[ACGT]AGG
937 CCTGAGG
HaeII 115 GGCGCT [GA]GCGC^[CT]
1242 GGCGCC
HgaI 235 GCGTC GACGC(5/10)
676 GCGTC
HphI 186 GGTGA GGTGA(8/7)
256 TCACC
MluNI 2 TGGCCA TGG^CCA
851 TGGCCA
MscI 2 TGGCCA TGG^CCA
851 TGGCCA
NaeI 1239 GCCGGC GCC^GGC
1475 GCCGGC
NgoMIV 1239 GCCGGC G^CCGGC
1475 GCCGGC
NlaIII 823 CATG CATG^
1164 CATG
PflMI 843 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1605 CCAAGAGATGG
SmaI 29 CCCGGG CCC^GGG
521 CCCGGG
TaqI 16 TCGA T^CGA
1055 TCGA
Tsp509I 691 AATT ^AATT
1652 AATT
TthHB8I 16 TCGA T^CGA
1055 TCGA
Van91I 843 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1605 CCAAGAGATGG
XmaCI 29 CCCGGG C^CCGGG
521 CCCGGG
XmaI 29 CCCGGG C^CCGGG
521 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaI AvaII BanI BbvI BcnI
BfaI BglI BmyI BpmI BsaJI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII ItaI MaeI MboI MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIV PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TseI
TspRI XcmI XhoII