##################################
# NO CUT ENZYMES (  210 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BbvI        BcgI        BciVI       BcnI        
BfrI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstNI       BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DdeI        DraI        DraII       
DraIII      DsaI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT14I     FauI        FokI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HpaI        HpaII       
KasI        KpnI        Ksp632I     KspI        MaeII       
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MslI        MspI        MunI        
MvaI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        ScrFI       SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Tru9I       TseI        
Tsp45I      TspRI       Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AciI           13  GCGG                 CCGC(-3/-1)
AcsI           72  AAATTT               [GA]^AATT[CT]
AfaI          270  GTAC                 GT^AC
AlwI          364  GATCC                GGATC(4/5)
ApoI           72  AAATTT               [GA]^AATT[CT]
AvaII         127  GGACC                G^G[AT]CC
BclI          222  TGATCA               T^GATCA
BfaI          167  CTAG                 C^TAG
BsmI          213  GAATGC               GAATGC(1/-1)
BstYI         363  AGATCC               [GA]^GATC[CT]
Cac8I           9  GCAAGC               GC[ACGT]^[ACGT]GC
Cfr13I        127  GGACC                G^G[ACGT]CC
DrdI          128  GACCTATTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI           14  CGGCCA               [CT]^GGCC[GA]
EcoT22I       215  ATGCAT               ATGCA^T
FbaI          222  TGATCA               T^GATCA
Fnu4HI         13  GCGGC                GC^[ACGT]GC
HaeIII         15  GGCC                 GG^CC
HindIII       331  AAGCTT               A^AGCTT
HinfI         236  GATTC                G^A[ACGT]TC
HphI          225  TCACC                GGTGA(8/7)
ItaI           13  GCGGC                GC^[ACGT]GC
MaeI          167  CTAG                 C^TAG
MaeIII        248  GTAAC                ^GT[ACGT]AC
MflI          363  AGATCC               [GA]^GATC[CT]
MspA1I         92  CAGCTG               C[AC]G^C[GT]G
NlaIII        218  CATG                 CATG^
NsiI          215  ATGCAT               ATGCA^T
NspI          217  GCATGT               [GA]CATG^[CT]
PvuII          92  CAGCTG               CAG^CTG
RsaI          270  GTAC                 GT^AC
Sau96I        127  GGACC                G^G[ACGT]CC
SmlI          337  CTCAAG               C^T[CT][GA]AG
TaqI           33  TCGA                 T^CGA
TfiI          236  GATTC                G^A[AT]TC
Tsp509I        73  AATT                 ^AATT
TthHB8I        33  TCGA                 T^CGA
XhoII         363  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    5 enzymes) #
###################################

BglI           16  GCCAAGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              280  GCCAACAGGGC         
DpnII         223  GATC                 ^GATC
              364  GATC                
MboI          223  GATC                 ^GATC
              364  GATC                
NdeII         223  GATC                 ^GATC
              364  GATC                
Sau3AI        223  GATC                 ^GATC
              364  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

AluI        MboII       MnlI        MwoI        SfaNI