Restriction map for
pF1KB5813
##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BamHI BbeI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MseI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SexAI SfoI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI TfiI Tru9I Tsp509I Tth111I
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 634 GACGTC GACGT^C
AccI 97 GTCGAC GT^[AC][GT]AC
AcyI 634 GACGTC G[GA]^CG[CT]C
AviII 581 TGCGCA TGC^GCA
BbsI 637 GTCTTC GAAGAC(2/6)
BbvCI 1228 GCTGAGG CCTCAGC(-5/-2)
BcgI 156 GCAGCTGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 82 CTAG C^TAG
BpuAI 637 GTCTTC GAAGAC(2/6)
BsaHI 634 GACGTC G[GA]^CG[CT]C
BsiEI 191 CGGCCG CG[GA][CT]^CG
BsmAI 1362 GAGAC GTCTC(1/5)
BspLU11I 440 ACATGT A^CATGT
BsrFI 193 GCCGGC [GA]^CCGG[CT]
BstAPI 1112 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 193 GCCGGC [GA]^CCGG[CT]
DraIII 547 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 191 CGGCCG C^GGCCG
EclXI 191 CGGCCG C^GGCCG
Eco52I 191 CGGCCG C^GGCCG
FspI 581 TGCGCA TGC^GCA
HaeII 77 AGCGCC [GA]GCGC^[CT]
Hin1I 634 GACGTC G[GA]^CG[CT]C
MaeI 82 CTAG C^TAG
MslI 990 CATCCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 193 GCCGGC GCC^GGC
NgoMIV 193 GCCGGC G^CCGGC
PflMI 546 CCACCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SalI 97 GTCGAC G^TCGAC
SbfI 758 CCTGCAGG CCTGCA^GG
ScaI 428 AGTACT AGT^ACT
SfiI 363 GGCCAAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 673 CTCAAG C^T[CT][GA]AG
Sse8387I 758 CCTGCAGG CCTGCA^GG
StuI 755 AGGCCT AGG^CCT
Tsp45I 1250 GTGAC ^GT[GC]AC
Van91I 546 CCACCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 46 CGCG CG^CG
285 CGCG
AlwNI 685 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1113 CAGCTGCTG
BbrPI 613 CACGTG CAC^GTG
994 CACGTG
BglII 1055 AGATCT A^GATCT
1320 AGATCT
BsaAI 613 CACGTG [CT]AC^GT[GA]
994 CACGTG
BsgI 403 CTGCAC GTGCAG(16/14)
564 CTGCAC
BsrDI 1304 CATTGC GCAATG(2/0)
90 GCAATG
BstUI 46 CGCG CG^CG
285 CGCG
BstYI 1055 AGATCT [GA]^GATC[CT]
1320 AGATCT
EcoNI 473 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
879 CCTGTCCCAGG
HgaI 95 GCGTC GACGC(5/10)
747 GCGTC
HincII 97 GTCGAC GT[CT]^[GA]AC
697 GTCAAC
HindII 97 GTCGAC GT[CT]^[GA]AC
697 GTCAAC
MaeIII 779 GTTAC ^GT[ACGT]AC
1250 GTGAC
MflI 1055 AGATCT [GA]^GATC[CT]
1320 AGATCT
MvnI 46 CGCG CG^CG
285 CGCG
NcoI 658 CCATGG C^CATGG
1028 CCATGG
NspI 389 ACATGC [GA]CATG^[CT]
440 ACATGT
PmaCI 613 CACGTG CAC^GTG
994 CACGTG
PmlI 613 CACGTG CAC^GTG
994 CACGTG
SapI 85 GAAGAGC GCTCTTC(1/4)
327 GCTCTTC
XhoII 1055 AGATCT [GA]^GATC[CT]
1320 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AfaI AflIII AluI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MboI MboII
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StyI TaqI
TseI TspRI TthHB8I XmaCI XmaI