Restriction map for
pF1KB5807
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
AspI AvaI AvrII BamHI BbeI
BbrPI BbvCI BciVI BclI BcnI
BfaI BfrI BlnI BsaAI BsaBI
BsaWI BseAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
EarI EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI HaeII
HincII HindII HindIII HpaI KasI
Ksp632I MaeI MamI MfeI MluI
MroI MseI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TaqI Tru9I Tsp509I Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 449 GGTACC G^GTACC
AcyI 1267 GACGCC G[GA]^CG[CT]C
AhdI 1083 GACCAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1363 GTGCAC G^TGCAC
AlwNI 1015 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 513 CCTGAGG CC^T[ACGT]AGG
ApaLI 1363 GTGCAC G^TGCAC
Asp718I 449 GGTACC G^GTACC
AspEI 1083 GACCAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 449 GGTACC G^G[CT][GA]CC
BbsI 1212 GAAGAC GAAGAC(2/6)
BcgI 1395 GCAGGTCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 577 GCCCGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 989 AGATCT A^GATCT
BlpI 1044 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1044 GCTCAGC GC^T[ACGT]AGC
BpuAI 1212 GAAGAC GAAGAC(2/6)
BsaHI 1267 GACGCC G[GA]^CG[CT]C
BsaI 1081 GAGACC GGTCTC(1/5)
BsiEI 369 CGACCG CG[GA][CT]^CG
BsrBI 975 GAGCGG CCGCTC(-3/-3)
BstEII 348 GGTGACC G^GT[ACGT]ACC
BstPI 348 GGTGACC G^GT[ACGT]ACC
Bsu36I 513 CCTGAGG CC^T[ACGT]AGG
CelII 1044 GCTCAGC GC^T[ACGT]AGC
DsaI 587 CCGCGG C^C[GA][CT]GG
Eam1105I 1083 GACCAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 513 CCTGAGG CC^T[ACGT]AGG
EcoO65I 348 GGTGACC G^GT[ACGT]ACC
FauI 164 CCCGC CCCGC(4/6)
Hin1I 1267 GACGCC G[GA]^CG[CT]C
HinfI 525 GATTC G^A[ACGT]TC
KpnI 449 GGTACC GGTAC^C
KspI 587 CCGCGG CCGC^GG
MaeII 1208 ACGT A^CGT
NspI 1100 GCATGC [GA]CATG^[CT]
PshAI 481 GACTACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 997 GAGCTC GAGCT^C
SacII 587 CCGCGG CCGC^GG
SphI 1100 GCATGC GCATG^C
TfiI 525 GATTC G^A[AT]TC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AfaI 450 GTAC GT^AC
1191 GTAC
AviII 629 TGCGCA TGC^GCA
662 TGCGCA
BalI 689 TGGCCA TGG^CCA
950 TGGCCA
BanII 45 GGGCTC G[GA]GC[CT]^C
997 GAGCTC
BsgI 821 CTGCAC GTGCAG(16/14)
970 GTGCAG
BsmFI 488 GTCCC GGGAC(10/14)
1038 GTCCC
BsmI 771 GAATGC GAATGC(1/-1)
845 GCATTC
BssSI 757 CACGAG CACGAG(-5/-1)
1390 CACGAG
BstXI 786 CCAGGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1349 CCAAGGTCATGG
EcoT14I 273 CCTTGG C^C[AT][AT]GG
1349 CCAAGG
FspI 629 TGCGCA TGC^GCA
662 TGCGCA
HapII 107 CCGG C^CGG
1002 CCGG
HpaII 107 CCGG C^CGG
1002 CCGG
MluNI 689 TGGCCA TGG^CCA
950 TGGCCA
MscI 689 TGGCCA TGG^CCA
950 TGGCCA
MslI 633 CATGAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1356 CATGGATGTG
MspI 107 CCGG C^CGG
1002 CCGG
PvuII 1105 CAGCTG CAG^CTG
1147 CAGCTG
RsaI 450 GTAC GT^AC
1191 GTAC
StyI 273 CCTTGG C^C[AT][AT]GG
1349 CCAAGG
TspRI 339 CAGTG CA[GC]TG[ACGT][ACGT]^
539 CAGTG
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
AvaII BbvI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoNI EcoO109I EcoRII Fnu4HI FokI
HaeIII HgaI HhaI HinP1I HphI
ItaI MaeIII MboI MboII MflI
MnlI MspA1I MvaI MvnI MwoI
NdeII NlaIII NlaIV PpuMI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI Tsp45I XcmI XhoII