Restriction map for
pF1KB5801
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BanI
BbeI BbrPI BbvCI BciVI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrGI BssHII Bst1107I BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HaeII Hin1I HincII HindII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MspA1I MunI NarI NdeI NheI
NotI NruI NsiI PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AflIII 614 ACATGT A^C[GA][CT]GT
AlwNI 987 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 527 GAGCTC G[AT]GC[AT]^C
BglII 27 AGATCT A^GATCT
BpmI 684 CTCCAG CTGGAG(16/14)
BsaI 471 GAGACC GGTCTC(1/5)
BsgI 189 GTGCAG GTGCAG(16/14)
BsiHKAI 527 GAGCTC G[AT]GC[AT]^C
BsmAI 471 GAGAC GTCTC(1/5)
BspLU11I 614 ACATGT A^CATGT
BspMI 665 ACCTGC ACCTGC(4/8)
BsrFI 995 GCCGGC [GA]^CCGG[CT]
BssSI 144 CACGAG CACGAG(-5/-1)
BstBI 902 TTCGAA TT^CGAA
CfoI 232 GCGC GCG^C
Cfr10I 995 GCCGGC [GA]^CCGG[CT]
DraI 357 TTTAAA TTT^AAA
EcoRI 288 GAATTC G^AATTC
HhaI 232 GCGC GCG^C
HinP1I 232 GCGC G^CGC
HindIII 411 AAGCTT A^AGCTT
MaeII 755 ACGT A^CGT
MslI 615 CATGTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 995 GCCGGC GCC^GGC
NgoMIV 995 GCCGGC G^CCGGC
NspV 902 TTCGAA TT^CGAA
PaeR7I 770 CTCGAG C^TCGAG
PshAI 1020 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 527 GAGCTC GAGCT^C
SexAI 541 ACCAGGT A^CC[AT]GGT
SfaNI 931 GATGC GCATC(5/9)
SfuI 902 TTCGAA TT^CGAA
SmaI 492 CCCGGG CCC^GGG
SmlI 770 CTCGAG C^T[CT][GA]AG
SphI 1068 GCATGC GCATG^C
XhoI 770 CTCGAG C^TCGAG
XmaCI 492 CCCGGG C^CCGGG
XmaI 492 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BamHI 557 GGATCC G^GATCC
942 GGATCC
BanII 527 GAGCTC G[GA]GC[CT]^C
798 GGGCTC
BmyI 527 GAGCTC G[AGT]GC[ACT]^C
798 GGGCTC
Bsp1286I 527 GAGCTC G[AGT]GC[ACT]^C
798 GGGCTC
BsrDI 955 CATTGC GCAATG(2/0)
379 GCAATG
BstXI 859 CCAGACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
882 CCAGTTCTGTGG
EarI 601 GAAGAG CTCTTC(1/4)
919 GAAGAG
EcoNI 964 CCTTGTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1086 CCTGAAACAGG
EcoT14I 80 CCATGG C^C[AT][AT]GG
584 CCATGG
HaeIII 880 GGCC GG^CC
892 GGCC
HgaI 4 GACGC GACGC(5/10)
574 GACGC
HphI 621 GGTGA GGTGA(8/7)
916 GGTGA
Ksp632I 601 GAAGAG CTCTTC(1/4)
919 GAAGAG
MseI 49 TTAA T^TAA
358 TTAA
NcoI 80 CCATGG C^CATGG
584 CCATGG
NspI 614 ACATGT [GA]CATG^[CT]
1068 GCATGC
PleI 457 GACTC GAGTC(4/5)
268 GAGTC
StyI 80 CCATGG C^C[AT][AT]GG
584 CCATGG
Tru9I 49 TTAA T^TAA
358 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaI AvaII BbsI
BbvI BcgI BcnI BpuAI BsaJI
BseRI BsiYI BslI BsmFI BsoBI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
FauI Fnu4HI FokI HapII HinfI
HpaII ItaI MaeIII MboI MboII
MflI MnlI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfcI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII