Restriction map for
pF1KB7987
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII AccI AclI AcsI AflII
AflIII AgeI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I AviII
AvrII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsiWI BsmI Bsp1407I BspDI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KasI MaeI MfeI MluI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SalI
ScaI SexAI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI SspBI
SspI SwaI TfiI Tru9I Tsp509I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 1142 GGTACC G^GTACC
AccIII 287 TCCGGA T^CCGGA
Asp718I 1142 GGTACC G^GTACC
AspI 542 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1142 GGTACC G^G[CT][GA]CC
BglI 145 GCCACTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 256 CTGGAG CTGGAG(16/14)
BsaBI 123 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 287 TCCGGA [AT]^CCGG[AT]
BseAI 287 TCCGGA T^CCGGA
BsmBI 379 GAGACG CGTCTC(1/5)
BspEI 287 TCCGGA T^CCGGA
BspHI 56 TCATGA T^CATGA
BsrBI 634 GAGCGG CCGCTC(-3/-3)
BsrFI 419 ACCGGC [GA]^CCGG[CT]
BstAPI 669 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 419 ACCGGC [GA]^CCGG[CT]
DdeI 1115 CTGAG C^T[ACGT]AG
HincII 1051 GTCAAC GT[CT]^[GA]AC
HindII 1051 GTCAAC GT[CT]^[GA]AC
KpnI 1142 GGTACC GGTAC^C
MamI 123 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 287 TCCGGA T^CCGGA
PflFI 542 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1076 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1184 CAGCTG CAG^CTG
RcaI 56 TCATGA T^CATGA
SacI 1117 GAGCTC GAGCT^C
SbfI 666 CCTGCAGG CCTGCA^GG
SfiI 144 GGCCACTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 794 CCCGGG CCC^GGG
Sse8387I 666 CCTGCAGG CCTGCA^GG
StuI 581 AGGCCT AGG^CCT
Tth111I 542 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1076 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 245 CCAGGGAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 794 CCCGGG C^CCGGG
XmaI 794 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AhdI 163 GACGCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
316 GACATCAAGTC
Alw44I 871 GTGCAC G^TGCAC
1108 GTGCAC
ApaLI 871 GTGCAC G^TGCAC
1108 GTGCAC
AspEI 163 GACGCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
316 GACATCAAGTC
AvaI 794 CCCGGG C^[CT]CG[GA]G
1288 CCCGAG
BalI 143 TGGCCA TGG^CCA
1272 TGGCCA
BsaI 523 GAGACC GGTCTC(1/5)
1301 GAGACC
BseRI 376 GAGGAG GAGGAG(10/8)
985 GAGGAG
BsiEI 326 CGGCCG CG[GA][CT]^CG
383 CGGCCG
BsoBI 794 CCCGGG C^[CT]CG[GA]G
1288 CCCGAG
BspMI 674 ACCTGC ACCTGC(4/8)
1139 GCAGGT
BsrDI 92 CATTGC GCAATG(2/0)
342 CATTGC
BssSI 461 CACGAG CACGAG(-5/-1)
874 CACGAG
DrdI 745 GACTTCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1096 GACCCCGCCGTC
EagI 326 CGGCCG C^GGCCG
383 CGGCCG
Eam1105I 163 GACGCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
316 GACATCAAGTC
EarI 601 CTCTTC CTCTTC(1/4)
679 CTCTTC
EclXI 326 CGGCCG C^GGCCG
383 CGGCCG
Eco52I 326 CGGCCG C^GGCCG
383 CGGCCG
EcoT14I 527 CCTTGG C^C[AT][AT]GG
578 CCAAGG
Ksp632I 601 CTCTTC CTCTTC(1/4)
679 CTCTTC
MaeIII 697 GTGAC ^GT[ACGT]AC
1313 GTGAC
MluNI 143 TGGCCA TGG^CCA
1272 TGGCCA
MscI 143 TGGCCA TGG^CCA
1272 TGGCCA
MslI 972 CATCGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1306 CACGCCAGTG
PstI 649 CTGCAG CTGCA^G
667 CTGCAG
SapI 600 GCTCTTC GCTCTTC(1/4)
678 GCTCTTC
StyI 527 CCTTGG C^C[AT][AT]GG
578 CCAAGG
Tsp45I 697 GTGAC ^GT[GC]AC
1313 GTGAC
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaII
BamHI BanII BbvI BcnI BmyI
BsaHI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DpnII DraII
DsaI EaeI EcoNI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KspI
MaeII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
TspRI TthHB8I XhoII