Restriction map for
pF1KB9923
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tth111I Van91I XbaI XcmI
XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AccII 93 CGCG CG^CG
AlwNI 832 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
BanI 513 GGTGCC G^G[CT][GA]CC
BpmI 874 CTCCAG CTGGAG(16/14)
BsaI 179 GAGACC GGTCTC(1/5)
BsmBI 887 GAGACG CGTCTC(1/5)
BsrBI 283 GAGCGG CCGCTC(-3/-3)
BsrFI 257 GCCGGT [GA]^CCGG[CT]
BstAPI 897 GCACGTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 381 GGTGACC G^GT[ACGT]ACC
BstPI 381 GGTGACC G^GT[ACGT]ACC
BstUI 93 CGCG CG^CG
Cfr10I 257 GCCGGT [GA]^CCGG[CT]
EaeI 255 TGGCCG [CT]^GGCC[GA]
EcoO65I 381 GGTGACC G^GT[ACGT]ACC
HaeII 856 GGCGCT [GA]GCGC^[CT]
MaeII 899 ACGT A^CGT
MseI 569 TTAA T^TAA
MslI 684 CACCCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 965 CAGCTG C[AC]G^C[GT]G
MvnI 93 CGCG CG^CG
PstI 482 CTGCAG CTGCA^G
PvuII 965 CAGCTG CAG^CTG
SacI 820 GAGCTC GAGCT^C
SfaNI 592 GCATC GCATC(5/9)
SfcI 482 CTGCAG C^T[GA][CT]AG
SmaI 239 CCCGGG CCC^GGG
Tru9I 569 TTAA T^TAA
Tsp509I 506 AATT ^AATT
XmaCI 239 CCCGGG C^CCGGG
XmaI 239 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
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Alw44I 322 GTGCAC G^TGCAC
895 GTGCAC
ApaI 242 GGGCCC GGGCC^C
787 GGGCCC
ApaLI 322 GTGCAC G^TGCAC
895 GTGCAC
BglI 544 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
750 GCCAGGAGGGC
BlpI 628 GCTGAGC GC^T[ACGT]AGC
694 GCTGAGC
Bpu1102I 628 GCTGAGC GC^T[ACGT]AGC
694 GCTGAGC
BsgI 869 CTGCAC GTGCAG(16/14)
1003 CTGCAC
BspMI 305 ACCTGC ACCTGC(4/8)
330 ACCTGC
BsrI 517 CCAGT ACTGG(1/-1)
920 CCAGT
BstXI 246 CCAGGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
683 CCACCCATGTGG
BstYI 558 GGATCT [GA]^GATC[CT]
992 AGATCC
CelII 628 GCTGAGC GC^T[ACGT]AGC
694 GCTGAGC
MflI 558 GGATCT [GA]^GATC[CT]
992 AGATCC
NlaIII 175 CATG CATG^
688 CATG
PaeR7I 217 CTCGAG C^TCGAG
499 CTCGAG
Tsp45I 382 GTGAC ^GT[GC]AC
923 GTGAC
XhoI 217 CTCGAG C^TCGAG
499 CTCGAG
XhoII 558 GGATCT [GA]^GATC[CT]
992 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BanII BbvI BcnI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIV PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SmlI StyI TaqI
TseI TspRI TthHB8I