Restriction map for
pF1KB7971
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# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BalI BanI BbeI BbrPI
BbvCI BcgI BfaI BfrI BglI
BglII BlnI BsaAI BsaBI BsaHI
BsaI BsiEI BsiWI BsmAI BsmBI
BsmFI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaI KasI
KpnI KspI MaeI MaeII MamI
MfeI MluI MluNI MscI MspA1I
MunI MvnI MwoI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 143 GTAGAC GT^[AC][GT]AC
AccIII 71 TCCGGA T^CCGGA
AciI 283 GCGG CCGC(-3/-1)
AcsI 130 GAATTT [GA]^AATT[CT]
AfaI 390 GTAC GT^AC
ApaI 610 GGGCCC GGGCC^C
ApoI 130 GAATTT [GA]^AATT[CT]
AspI 670 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 91 CCCGAG C^[CT]CG[GA]G
BbsI 226 GAAGAC GAAGAC(2/6)
BbvI 212 GCAGC GCAGC(8/12)
BciVI 190 GTATCC GTATCC(6/5)
BclI 162 TGATCA T^GATCA
BlpI 33 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 33 GCTCAGC GC^T[ACGT]AGC
BpuAI 226 GAAGAC GAAGAC(2/6)
BsaWI 71 TCCGGA [AT]^CCGG[AT]
BseAI 71 TCCGGA T^CCGGA
BsgI 298 CTGCAC GTGCAG(16/14)
BsmI 293 GCATTC GAATGC(1/-1)
BsoBI 91 CCCGAG C^[CT]CG[GA]G
BspEI 71 TCCGGA T^CCGGA
CelII 33 GCTCAGC GC^T[ACGT]AGC
DraI 133 TTTAAA TTT^AAA
DraII 4 GGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 333 CCATGG C^C[GA][CT]GG
EarI 784 GAAGAG CTCTTC(1/4)
EcoO109I 4 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 588 GATATC GAT^ATC
FauI 283 GCGGG CCCGC(4/6)
FbaI 162 TGATCA T^GATCA
Fnu4HI 212 GCAGC GC^[ACGT]GC
HindIII 573 AAGCTT A^AGCTT
HphI 667 GGTGA GGTGA(8/7)
ItaI 212 GCAGC GC^[ACGT]GC
Ksp632I 784 GAAGAG CTCTTC(1/4)
MroI 71 TCCGGA T^CCGGA
MslI 648 CACAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 333 CCATGG C^CATGG
PflFI 670 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 4 GGGTCCT [GA]G^G[AT]CC[CT]
RsaI 390 GTAC GT^AC
SacI 363 GAGCTC GAGCT^C
SmlI 794 CTCAAG C^T[CT][GA]AG
TaqI 174 TCGA T^CGA
TseI 212 GCAGC G^C[AT]GC
Tth111I 670 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 174 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwNI 394 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
443 CAGTTGCTG
AspHI 31 GTGCTC G[AT]GC[AT]^C
363 GAGCTC
BamHI 108 GGATCC G^GATCC
693 GGATCC
BanII 363 GAGCTC G[GA]GC[CT]^C
610 GGGCCC
BcnI 39 CCGGG CC^[GC]GG
288 CCGGG
BpmI 458 CTGGAG CTGGAG(16/14)
593 CTGGAG
BseRI 322 GAGGAG GAGGAG(10/8)
360 GAGGAG
BsiHKAI 31 GTGCTC G[AT]GC[AT]^C
363 GAGCTC
BstYI 108 GGATCC [GA]^GATC[CT]
693 GGATCC
EcoT14I 333 CCATGG C^C[AT][AT]GG
482 CCAAGG
MflI 108 GGATCC [GA]^GATC[CT]
693 GGATCC
NciI 39 CCGGG CC^[GC]GG
288 CCGGG
PflMI 328 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
842 CCAAAACCTGG
SfaNI 309 GATGC GCATC(5/9)
619 GATGC
StyI 333 CCATGG C^C[AT][AT]GG
482 CCAAGG
Tsp45I 439 GTGAC ^GT[GC]AC
668 GTGAC
Van91I 328 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
842 CCAAAACCTGG
XhoII 108 GGATCC [GA]^GATC[CT]
693 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AluI AlwI AvaII BmyI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII FokI HaeIII HapII
HpaII MaeIII MboI MboII MnlI
MseI MspI MvaI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI Tru9I
Tsp509I TspRI