Restriction map for
pF1KB7950
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AocI
Aor51HI ApaI AseI AsnI Asp718I
AspEI AspI AviII AvrII BbrPI
BbsI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstBI BstXI BstZ17I
Bsu36I CelII ClaI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MaeI
MamI MfeI MluI MroI MseI
MunI NcoI NdeI NheI NotI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
SacI SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tth111I Van91I
XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
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AcsI 184 AAATTC [GA]^AATT[CT]
AfaI 105 GTAC GT^AC
Alw44I 291 GTGCAC G^TGCAC
AlwNI 625 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 291 GTGCAC G^TGCAC
ApoI 184 AAATTC [GA]^AATT[CT]
AscI 436 GGCGCGCC GG^CGCGCC
AspHI 291 GTGCAC G[AT]GC[AT]^C
BalI 1022 TGGCCA TGG^CCA
BamHI 678 GGATCC G^GATCC
BanII 570 GAGCCC G[GA]GC[CT]^C
BbvCI 999 GCTGAGG CCTCAGC(-5/-2)
BsaWI 295 ACCGGA [AT]^CCGG[AT]
BseRI 835 GAGGAG GAGGAG(10/8)
BsgI 480 CTGCAC GTGCAG(16/14)
BsiEI 420 CGACCG CG[GA][CT]^CG
BsiHKAI 291 GTGCAC G[AT]GC[AT]^C
BsmBI 299 GAGACG CGTCTC(1/5)
BspMI 977 ACCTGC ACCTGC(4/8)
BssHII 437 GCGCGC G^CGCGC
BstAPI 1143 GCAGCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 648 GGTCACC G^GT[ACGT]ACC
BstPI 648 GGTCACC G^GT[ACGT]ACC
BstYI 678 GGATCC [GA]^GATC[CT]
CpoI 48 CGGTCCG CG^G[AT]CCG
EaeI 1022 TGGCCA [CT]^GGCC[GA]
EcoNI 1056 CCTAAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 648 GGTCACC G^GT[ACGT]ACC
HgaI 463 GCGTC GACGC(5/10)
HphI 650 TCACC GGTGA(8/7)
MaeII 211 ACGT A^CGT
MflI 678 GGATCC [GA]^GATC[CT]
MluNI 1022 TGGCCA TGG^CCA
MscI 1022 TGGCCA TGG^CCA
NruI 985 TCGCGA TCG^CGA
NspI 371 GCATGT [GA]CATG^[CT]
PstI 815 CTGCAG CTGCA^G
PvuII 1026 CAGCTG CAG^CTG
RsaI 105 GTAC GT^AC
RsrII 48 CGGTCCG CG^G[AT]CCG
SexAI 1007 ACCAGGT A^CC[AT]GGT
TspRI 1173 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 678 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 438 CGCG CG^CG
986 CGCG
BbeI 159 GGCGCC GGCGC^C
510 GGCGCC
BsmAI 299 GAGAC GTCTC(1/5)
627 GTCTC
BsrI 1174 ACTGG ACTGG(1/-1)
885 CCAGT
BstUI 438 CGCG CG^CG
986 CGCG
DpnII 679 GATC ^GATC
1053 GATC
DsaI 155 CCACGG C^C[GA][CT]GG
277 CCGTGG
KasI 159 GGCGCC G^GCGCC
510 GGCGCC
MboI 679 GATC ^GATC
1053 GATC
MslI 654 CATCACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
724 CACAACTATG
MspA1I 529 CCGCTG C[AC]G^C[GT]G
1026 CAGCTG
MvnI 438 CGCG CG^CG
986 CGCG
NarI 159 GGCGCC GG^CGCC
510 GGCGCC
NdeII 679 GATC ^GATC
1053 GATC
Sau3AI 679 GATC ^GATC
1053 GATC
SfaNI 811 GATGC GCATC(5/9)
1133 GATGC
SfoI 159 GGCGCC GGC^GCC
510 GGCGCC
StuI 551 AGGCCT AGG^CCT
1064 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcyI AluI AlwI Asp700I
AvaI AvaII BanI BbvI BcnI
BmyI BpmI BsaHI BsaJI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BssKI BstF5I BstNI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
ItaI Ksp632I MaeIII MboII MnlI
MspI MvaI MwoI NaeI NciI
NgoMIV NlaIII NlaIV PleI PpuMI
Sau96I ScrFI SfcI SmaI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TthHB8I XmaCI XmaI XmnI