Restriction map for
pF1KB7947
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# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
Ksp632I MaeI MaeII MaeIII MamI
MfeI MluI MluNI MroI MscI
MseI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp45I Tsp509I Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccII 9 CGCG CG^CG
AcyI 298 GACGCC G[GA]^CG[CT]C
AfaI 174 GTAC GT^AC
BamHI 3 GGATCC G^GATCC
BsaHI 298 GACGCC G[GA]^CG[CT]C
BsrDI 86 GCAATG GCAATG(2/0)
BsrI 278 ACTGG ACTGG(1/-1)
BstAPI 562 GCAGAGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 65 TTCGAA TT^CGAA
BstUI 9 CGCG CG^CG
BstYI 3 GGATCC [GA]^GATC[CT]
DpnII 4 GATC ^GATC
DsaI 8 CCGCGG C^C[GA][CT]GG
EaeI 11 CGGCCA [CT]^GGCC[GA]
EcoNI 219 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 635 AGCGCC [GA]GCGC^[CT]
HgaI 298 GACGC GACGC(5/10)
Hin1I 298 GACGCC G[GA]^CG[CT]C
HphI 533 TCACC GGTGA(8/7)
KspI 8 CCGCGG CCGC^GG
MboI 4 GATC ^GATC
MflI 3 GGATCC [GA]^GATC[CT]
MvnI 9 CGCG CG^CG
NdeII 4 GATC ^GATC
NlaIII 24 CATG CATG^
NspV 65 TTCGAA TT^CGAA
PpuMI 719 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 174 GTAC GT^AC
SacII 8 CCGCGG CCGC^GG
Sau3AI 4 GATC ^GATC
SfuI 65 TTCGAA TT^CGAA
TaqI 66 TCGA T^CGA
TthHB8I 66 TCGA T^CGA
XcmI 43 CCAGCCCCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 3 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
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AlwI 4 GATCC GGATC(4/5)
3 GGATC
AlwNI 15 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
44 CAGCCCCTG
AocI 95 CCTCAGG CC^T[ACGT]AGG
724 CCTGAGG
AspHI 232 GAGCAC G[AT]GC[AT]^C
414 GAGCAC
BcnI 302 CCCGG CC^[GC]GG
324 CCCGG
BmyI 232 GAGCAC G[AGT]GC[ACT]^C
414 GAGCAC
BpmI 34 CTCCAG CTGGAG(16/14)
556 CTGGAG
BseRI 27 GAGGAG GAGGAG(10/8)
229 GAGGAG
BsiHKAI 232 GAGCAC G[AT]GC[AT]^C
414 GAGCAC
BsmAI 427 GAGAC GTCTC(1/5)
292 GTCTC
Bsp1286I 232 GAGCAC G[AGT]GC[ACT]^C
414 GAGCAC
BstXI 338 CCAAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
353 CCAGCAGCCTGG
Bsu36I 95 CCTCAGG CC^T[ACGT]AGG
724 CCTGAGG
CfoI 306 GCGC GCG^C
636 GCGC
DraII 91 GGGGCCT [GA]G^G[ACGT]CC[CT]
719 AGGACCC
Eco81I 95 CCTCAGG CC^T[ACGT]AGG
724 CCTGAGG
EcoO109I 91 GGGGCCT [GA]G^G[ACGT]CC[CT]
719 AGGACCC
EcoT14I 338 CCAAGG C^C[AT][AT]GG
536 CCAAGG
HhaI 306 GCGC GCG^C
636 GCGC
HinP1I 306 GCGC G^CGC
636 GCGC
HinfI 588 GACTC G^A[ACGT]TC
736 GACTC
MboII 435 GAAGA GAAGA(8/7)
239 TCTTC
NciI 302 CCCGG CC^[GC]GG
324 CCCGG
PleI 588 GACTC GAGTC(4/5)
736 GACTC
PvuII 18 CAGCTG CAG^CTG
544 CAGCTG
StyI 338 CCAAGG C^C[AT][AT]GG
536 CCAAGG
TspRI 243 CACTG CA[GC]TG[ACGT][ACGT]^
417 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AluI AvaII BbvI BsaJI
BsiYI BslI BsmFI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HpaII ItaI MnlI MspA1I MspI
MvaI MwoI NlaIV Sau96I ScrFI
TseI