Restriction map for
pF1KB7944
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaII AviII AvrII BbeI
BbrPI BbvCI BciVI BclI BfaI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BseAI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrFI
BsrGI BsrI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI MaeI MamI MfeI
MluI MroI MseI MslI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 235 GACGTC GACGT^C
AcyI 235 GACGTC G[GA]^CG[CT]C
AfaI 3 GTAC GT^AC
AocI 265 CCTCAGG CC^T[ACGT]AGG
ApaI 69 GGGCCC GGGCC^C
AvaI 66 CCCGGG C^[CT]CG[GA]G
BalI 312 TGGCCA TGG^CCA
BamHI 118 GGATCC G^GATCC
BbsI 481 GAAGAC GAAGAC(2/6)
BcgI 224 GCACTGACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 23 GCCCCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 701 AGATCT A^GATCT
BpuAI 481 GAAGAC GAAGAC(2/6)
BsaHI 235 GACGTC G[GA]^CG[CT]C
BsaWI 380 ACCGGA [AT]^CCGG[AT]
BsgI 285 CTGCAC GTGCAG(16/14)
BsmFI 238 GTCCC GGGAC(10/14)
BsoBI 66 CCCGGG C^[CT]CG[GA]G
BspMI 638 ACCTGC ACCTGC(4/8)
BsrBI 12 GAGCGG CCGCTC(-3/-3)
BsrDI 191 GCAATG GCAATG(2/0)
BssSI 588 CTCGTG CACGAG(-5/-1)
BstAPI 707 GCATGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 186 CCAAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 265 CCTCAGG CC^T[ACGT]AGG
DraII 212 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 686 CCGCGG C^C[GA][CT]GG
EarI 426 GAAGAG CTCTTC(1/4)
Eco81I 265 CCTCAGG CC^T[ACGT]AGG
EcoNI 159 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 212 AGGGCCT [GA]G^G[ACGT]CC[CT]
FauI 346 GCGGG CCCGC(4/6)
HaeII 20 AGCGCC [GA]GCGC^[CT]
Hin1I 235 GACGTC G[GA]^CG[CT]C
Ksp632I 426 GAAGAG CTCTTC(1/4)
KspI 686 CCGCGG CCGC^GG
MaeII 236 ACGT A^CGT
MluNI 312 TGGCCA TGG^CCA
MscI 312 TGGCCA TGG^CCA
MspA1I 686 CCGCGG C[AC]G^C[GT]G
NspI 256 ACATGC [GA]CATG^[CT]
PflMI 210 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 573 CTGCAG CTGCA^G
RsaI 3 GTAC GT^AC
SacII 686 CCGCGG CCGC^GG
SexAI 5 ACCAGGT A^CC[AT]GGT
SfcI 573 CTGCAG C^T[GA][CT]AG
SmaI 66 CCCGGG CCC^GGG
TaqI 233 TCGA T^CGA
Tsp45I 253 GTGAC ^GT[GC]AC
TthHB8I 233 TCGA T^CGA
Van91I 210 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 18 CCAGCGCCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 66 CCCGGG C^CCGGG
XmaI 66 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 39 CGCG CG^CG
687 CGCG
AlwNI 211 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
245 CAGAAGCTG
BanI 334 GGCACC G^G[CT][GA]CC
355 GGTGCC
BcnI 66 CCCGG CC^[GC]GG
67 CCGGG
BstUI 39 CGCG CG^CG
687 CGCG
DdeI 266 CTCAG C^T[ACGT]AG
321 CTCAG
DrdI 229 GACCTCGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
484 GACTGCTGTGTC
EaeI 15 CGGCCA [CT]^GGCC[GA]
312 TGGCCA
HapII 67 CCGG C^CGG
381 CCGG
HgaI 37 GACGC GACGC(5/10)
531 GACGC
HpaII 67 CCGG C^CGG
381 CCGG
HphI 9 GGTGA GGTGA(8/7)
126 TCACC
MaeIII 112 GTAAC ^GT[ACGT]AC
253 GTGAC
MboII 426 GAAGA GAAGA(8/7)
481 GAAGA
MspI 67 CCGG C^CGG
381 CCGG
MvnI 39 CGCG CG^CG
687 CGCG
NciI 66 CCCGG CC^[GC]GG
67 CCGGG
TspRI 225 CACTG CA[GC]TG[ACGT][ACGT]^
496 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AlwI BanII BbvI
BmyI BpmI BsaJI BseRI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HhaI HinP1I HinfI ItaI MboI
MflI MnlI MvaI MwoI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI StyI TseI XhoII