Restriction map for
pF1KB7935
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BsaAI BsaBI BseAI BsgI
BsiEI BsiWI BsmI BspDI BspEI
BspLU11I BsrBI BsrDI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FspI HincII HindII HindIII
HpaI HphI KpnI KspI MamI
MluI MluNI MroI MscI MseI
MslI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PstI
PvuI RsrII SacII SalI SbfI
SexAI SfaNI SfcI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
Tru9I Tth111I Van91I XbaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 583 GACGTC GACGT^C
AccI 1051 GTCTAC GT^[AC][GT]AC
AccII 804 CGCG CG^CG
ApaI 469 GGGCCC GGGCC^C
Asp700I 1090 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 781 CTCGAG C^[CT]CG[GA]G
BbeI 295 GGCGCC GGCGC^C
BfaI 460 CTAG C^TAG
BsaI 377 GAGACC GGTCTC(1/5)
BsaWI 634 TCCGGT [AT]^CCGG[AT]
BsmBI 585 CGTCTC CGTCTC(1/5)
BsoBI 781 CTCGAG C^[CT]CG[GA]G
Bsp1407I 53 TGTACA T^GTACA
BspHI 875 TCATGA T^CATGA
BspMI 210 GCAGGT ACCTGC(4/8)
BsrGI 53 TGTACA T^GTACA
BstUI 804 CGCG CG^CG
BstXI 170 CCAGCTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 278 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1038 TGGCCG [CT]^GGCC[GA]
FseI 742 GGCCGGCC GGCCGG^CC
KasI 295 GGCGCC G^GCGCC
MaeI 460 CTAG C^TAG
MfeI 309 CAATTG C^AATTG
MunI 309 CAATTG C^AATTG
MvnI 804 CGCG CG^CG
NarI 295 GGCGCC GG^CGCC
PaeR7I 781 CTCGAG C^TCGAG
PshAI 511 GACGACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 875 TCATGA T^CATGA
ScaI 767 AGTACT AGT^ACT
SfoI 295 GGCGCC GGC^GCC
SspBI 53 TGTACA T^GTACA
TfiI 888 GAATC G^A[AT]TC
Tsp509I 310 AATT ^AATT
XhoI 781 CTCGAG C^TCGAG
XmnI 1090 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AvaII 354 GGACC G^G[AT]CC
651 GGACC
BamHI 657 GGATCC G^GATCC
714 GGATCC
BanI 295 GGCGCC G^G[CT][GA]CC
814 GGCACC
BbsI 204 GAAGAC GAAGAC(2/6)
904 GTCTTC
BlpI 756 GCTGAGC GC^T[ACGT]AGC
984 GCTGAGC
BpmI 1099 CTCCAG CTGGAG(16/14)
427 CTGGAG
Bpu1102I 756 GCTGAGC GC^T[ACGT]AGC
984 GCTGAGC
BpuAI 204 GAAGAC GAAGAC(2/6)
904 GTCTTC
BseRI 624 CTCCTC GAGGAG(10/8)
322 GAGGAG
BsrFI 743 GCCGGC [GA]^CCGG[CT]
1040 GCCGGC
CelII 756 GCTGAGC GC^T[ACGT]AGC
984 GCTGAGC
Cfr10I 743 GCCGGC [GA]^CCGG[CT]
1040 GCCGGC
HaeII 262 AGCGCC [GA]GCGC^[CT]
295 GGCGCC
MaeII 584 ACGT A^CGT
647 ACGT
NaeI 743 GCCGGC GCC^GGC
1040 GCCGGC
NgoMIV 743 GCCGGC G^CCGGC
1040 GCCGGC
TaqI 202 TCGA T^CGA
782 TCGA
TspRI 45 CAGTG CA[GC]TG[ACGT][ACGT]^
663 CACTG
TthHB8I 202 TCGA T^CGA
782 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI BanII BbvI BcnI
BmyI BsaHI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SmlI TseI Tsp45I XhoII