Restriction map for
pF1KB6987
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssKI
BssSI Bst1107I BstBI BstEII BstNI
BstPI BstUI BstXI BstYI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRII EcoT14I EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeII MamI MfeI
MflI MluI MunI MvaI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScrFI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tsp45I Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccIII 68 TCCGGA T^CCGGA
AcsI 266 AAATTC [GA]^AATT[CT]
AlwI 259 GATCC GGATC(4/5)
AocI 538 CCTGAGG CC^T[ACGT]AGG
ApaI 443 GGGCCC GGGCC^C
ApoI 266 AAATTC [GA]^AATT[CT]
BalI 314 TGGCCA TGG^CCA
BbvI 226 GCTGC GCAGC(8/12)
BcgI 248 GCATCCGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 286 CTAG C^TAG
BpmI 577 CTCCAG CTGGAG(16/14)
BsaJI 109 CCTCGG C^C[ACGT][ACGT]GG
BsaWI 68 TCCGGA [AT]^CCGG[AT]
BseAI 68 TCCGGA T^CCGGA
BsgI 413 CTGCAC GTGCAG(16/14)
BsmAI 595 GTCTC GTCTC(1/5)
BsmFI 334 GGGAC GGGAC(10/14)
BsmI 234 GAATGC GAATGC(1/-1)
BspEI 68 TCCGGA T^CCGGA
BsrDI 381 CATTGC GCAATG(2/0)
BstF5I 249 CATCC GGATG(2/0)
Bsu36I 538 CCTGAGG CC^T[ACGT]AGG
CfoI 475 GCGC GCG^C
DpnII 259 GATC ^GATC
DraII 443 GGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 314 TGGCCA [CT]^GGCC[GA]
Eco81I 538 CCTGAGG CC^T[ACGT]AGG
EcoNI 51 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 443 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRV 100 GATATC GAT^ATC
FauI 508 CCCGC CCCGC(4/6)
FokI 249 CATCC GGATG(9/13)
HaeII 474 AGCGCC [GA]GCGC^[CT]
HapII 69 CCGG C^CGG
HhaI 475 GCGC GCG^C
HinP1I 475 GCGC G^CGC
HinfI 336 GACTC G^A[ACGT]TC
HpaII 69 CCGG C^CGG
HphI 500 TCACC GGTGA(8/7)
MaeI 286 CTAG C^TAG
MaeIII 613 GTAAC ^GT[ACGT]AC
MboI 259 GATC ^GATC
MboII 309 GAAGA GAAGA(8/7)
MluNI 314 TGGCCA TGG^CCA
MroI 68 TCCGGA T^CCGGA
MscI 314 TGGCCA TGG^CCA
MseI 140 TTAA T^TAA
MslI 306 CACGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 464 CAGCGG C[AC]G^C[GT]G
MspI 69 CCGG C^CGG
NdeI 395 CATATG CA^TATG
NdeII 259 GATC ^GATC
NspI 519 GCATGC [GA]CATG^[CT]
PleI 336 GACTC GAGTC(4/5)
PstI 629 CTGCAG CTGCA^G
Sau3AI 259 GATC ^GATC
ScaI 349 AGTACT AGT^ACT
SfaNI 248 GCATC GCATC(5/9)
SphI 519 GCATGC GCATG^C
TaqI 257 TCGA T^CGA
Tru9I 140 TTAA T^TAA
TseI 226 GCTGC G^C[AT]GC
TthHB8I 257 TCGA T^CGA
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# TWO CUT ENZYMES ( 10 enzymes) #
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AlwNI 221 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
407 CAGTGCCTG
AvaI 36 CCCGAG C^[CT]CG[GA]G
110 CTCGGG
AvaII 66 GGTCC G^G[AT]CC
71 GGACC
BsoBI 36 CCCGAG C^[CT]CG[GA]G
110 CTCGGG
BstAPI 220 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
229 GCAAAGAATGC
Fnu4HI 11 GCGGC GC^[ACGT]GC
226 GCTGC
ItaI 11 GCGGC GC^[ACGT]GC
226 GCTGC
NlaIII 520 CATG CATG^
653 CATG
SfcI 185 CTGTAG C^T[GA][CT]AG
629 CTGCAG
Tsp509I 267 AATT ^AATT
585 AATT
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AfaI AluI AspHI BanII
BmyI BseRI BsiHKAI BsiYI BslI
Bsp1286I BsrI Cac8I Cfr13I DdeI
HaeIII MnlI MwoI NlaIV RsaI
SacI Sau96I TspRI