Restriction map for
pF1KB6959
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII AccI AclI AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbrPI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrFI BsrGI BsrI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KspI MaeII
MamI MfeI MluI MluNI MscI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI Tru9I Tsp509I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 218 GGTACC G^GTACC
AccII 98 CGCG CG^CG
AccIII 554 TCCGGA T^CCGGA
ApaI 260 GGGCCC GGGCC^C
Asp718I 218 GGTACC G^GTACC
AspHI 325 GAGCTC G[AT]GC[AT]^C
AvaI 395 CCCGGG C^[CT]CG[GA]G
AvaII 557 GGACC G^G[AT]CC
BbvI 175 GCTGC GCAGC(8/12)
BcgI 125 GCACCACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 490 CTAG C^TAG
BglII 191 AGATCT A^GATCT
BsaI 457 GAGACC GGTCTC(1/5)
BsaWI 554 TCCGGA [AT]^CCGG[AT]
BseAI 554 TCCGGA T^CCGGA
BseRI 412 GAGGAG GAGGAG(10/8)
BsgI 251 GTGCAG GTGCAG(16/14)
BsiEI 350 CGATCG CG[GA][CT]^CG
BsiHKAI 325 GAGCTC G[AT]GC[AT]^C
BsmFI 197 GGGAC GGGAC(10/14)
BsoBI 395 CCCGGG C^[CT]CG[GA]G
BspEI 554 TCCGGA T^CCGGA
BsrBI 214 GAGCGG CCGCTC(-3/-3)
BsrDI 101 GCAATG GCAATG(2/0)
BssSI 364 CTCGTG CACGAG(-5/-1)
BstF5I 552 CATCC GGATG(2/0)
BstUI 98 CGCG CG^CG
BstXI 566 CCATCACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 191 AGATCT [GA]^GATC[CT]
CfoI 99 GCGC GCG^C
EcoNI 384 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 152 CCCGC CCCGC(4/6)
FokI 552 CATCC GGATG(9/13)
HhaI 99 GCGC GCG^C
HinP1I 99 GCGC G^CGC
HphI 230 TCACC GGTGA(8/7)
KpnI 218 GGTACC GGTAC^C
MaeI 490 CTAG C^TAG
MflI 191 AGATCT [GA]^GATC[CT]
MroI 554 TCCGGA T^CCGGA
MvnI 98 CGCG CG^CG
NheI 489 GCTAGC G^CTAGC
PleI 472 GACTC GAGTC(4/5)
PvuI 350 CGATCG CGAT^CG
PvuII 203 CAGCTG CAG^CTG
SacI 325 GAGCTC GAGCT^C
SapI 447 GCTCTTC GCTCTTC(1/4)
SmaI 395 CCCGGG CCC^GGG
TseI 175 GCTGC G^C[AT]GC
Tsp45I 380 GTGAC ^GT[GC]AC
XhoII 191 AGATCT [GA]^GATC[CT]
XmaCI 395 CCCGGG C^CCGGG
XmaI 395 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AfaI 219 GTAC GT^AC
240 GTAC
BanI 169 GGCACC G^G[CT][GA]CC
218 GGTACC
BanII 260 GGGCCC G[GA]GC[CT]^C
325 GAGCTC
BbsI 72 GAAGAC GAAGAC(2/6)
34 GTCTTC
BbvCI 384 CCTCAGC CCTCAGC(-5/-2)
461 CCTCAGC
BcnI 395 CCCGG CC^[GC]GG
396 CCGGG
BpmI 211 CTGGAG CTGGAG(16/14)
454 CTGGAG
BpuAI 72 GAAGAC GAAGAC(2/6)
34 GTCTTC
Cac8I 205 GCTGGC GC[ACGT]^[ACGT]GC
489 GCTAGC
EarI 448 CTCTTC CTCTTC(1/4)
615 GAAGAG
Fnu4HI 122 GCCGC GC^[ACGT]GC
175 GCTGC
HapII 396 CCGG C^CGG
555 CCGG
HpaII 396 CCGG C^CGG
555 CCGG
ItaI 122 GCCGC GC^[ACGT]GC
175 GCTGC
Ksp632I 448 CTCTTC CTCTTC(1/4)
615 GAAGAG
MaeIII 371 GTAAC ^GT[ACGT]AC
380 GTGAC
MslI 156 CACTGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
360 CATGCTCGTG
MspA1I 173 CCGCTG C[AC]G^C[GT]G
203 CAGCTG
MspI 396 CCGG C^CGG
555 CCGG
NciI 395 CCCGG CC^[GC]GG
396 CCGGG
NlaIII 337 CATG CATG^
360 CATG
RsaI 219 GTAC GT^AC
240 GTAC
SfaNI 551 GCATC GCATC(5/9)
629 GCATC
TfiI 58 GAATC G^A[AT]TC
92 GATTC
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AluI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BssKI BstNI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII HaeIII HinfI MboI MboII
MnlI MvaI MwoI NdeII NlaIV
Sau3AI Sau96I ScrFI TspRI