Restriction map for
pF1KB6253
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BbeI BbrPI BciVI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspHI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV FauI FseI FspI
Hin1I HphI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI PvuI
RcaI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AclI 338 AACGTT AA^CGTT
AflIII 795 ACATGT A^C[GA][CT]GT
Alw44I 1025 GTGCAC G^TGCAC
AlwNI 1815 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1025 GTGCAC G^TGCAC
Asp700I 1890 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1077 GGATCC G^GATCC
BbvCI 817 CCTCAGC CCTCAGC(-5/-2)
BcgI 1793 CGAGCCCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1502 TGATCA T^GATCA
BfaI 1480 CTAG C^TAG
BglII 1488 AGATCT A^GATCT
BpmI 765 CTCCAG CTGGAG(16/14)
BsaAI 1885 TACGTG [CT]AC^GT[GA]
BsaI 1310 GAGACC GGTCTC(1/5)
BseRI 1849 GAGGAG GAGGAG(10/8)
BsgI 226 GTGCAG GTGCAG(16/14)
Bsp1407I 318 TGTACA T^GTACA
BspLU11I 795 ACATGT A^CATGT
BsrDI 993 CATTGC GCAATG(2/0)
BsrGI 318 TGTACA T^GTACA
CfoI 1103 GCGC GCG^C
CpoI 330 CGGTCCG CG^G[AT]CCG
EcoRI 555 GAATTC G^AATTC
EcoT14I 280 CCTTGG C^C[AT][AT]GG
EcoT22I 1892 ATGCAT ATGCA^T
FbaI 1502 TGATCA T^GATCA
HaeII 1102 AGCGCC [GA]GCGC^[CT]
HhaI 1103 GCGC GCG^C
HinP1I 1103 GCGC G^CGC
HindIII 636 AAGCTT A^AGCTT
HpaI 718 GTTAAC GTT^AAC
MaeI 1480 CTAG C^TAG
NsiI 1892 ATGCAT ATGCA^T
PleI 601 GAGTC GAGTC(4/5)
PshAI 1908 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 338 AACGTT AA^CGTT
PvuII 824 CAGCTG CAG^CTG
RsrII 330 CGGTCCG CG^G[AT]CCG
SacI 265 GAGCTC GAGCT^C
ScaI 252 AGTACT AGT^ACT
SmlI 1427 CTCAAG C^T[CT][GA]AG
SspBI 318 TGTACA T^GTACA
SspI 1699 AATATT AAT^ATT
StyI 280 CCTTGG C^C[AT][AT]GG
XcmI 69 CCATGACTATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1890 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 555 GAATTC [GA]^AATT[CT]
1171 GAATTT
AlwI 1078 GATCC GGATC(4/5)
1077 GGATC
ApoI 555 GAATTC [GA]^AATT[CT]
1171 GAATTT
AspHI 265 GAGCTC G[AT]GC[AT]^C
1025 GTGCAC
AvaI 131 CCCGGG C^[CT]CG[GA]G
973 CCCGGG
BalI 629 TGGCCA TGG^CCA
705 TGGCCA
BanI 1138 GGCACC G^G[CT][GA]CC
1725 GGCACC
BanII 265 GAGCTC G[GA]GC[CT]^C
1794 GAGCCC
BsiHKAI 265 GAGCTC G[AT]GC[AT]^C
1025 GTGCAC
BsmI 1890 GAATGC GAATGC(1/-1)
1894 GCATTC
BsoBI 131 CCCGGG C^[CT]CG[GA]G
973 CCCGGG
BspMI 1827 ACCTGC ACCTGC(4/8)
936 GCAGGT
BstYI 1077 GGATCC [GA]^GATC[CT]
1488 AGATCT
DraII 276 GGGTCCT [GA]G^G[ACGT]CC[CT]
1783 AGGTCCC
EaeI 629 TGGCCA [CT]^GGCC[GA]
705 TGGCCA
EarI 136 GAAGAG CTCTTC(1/4)
445 GAAGAG
EcoO109I 276 GGGTCCT [GA]G^G[ACGT]CC[CT]
1783 AGGTCCC
HincII 718 GTTAAC GT[CT]^[GA]AC
909 GTCAAC
HindII 718 GTTAAC GT[CT]^[GA]AC
909 GTCAAC
Ksp632I 136 GAAGAG CTCTTC(1/4)
445 GAAGAG
MaeII 339 ACGT A^CGT
1886 ACGT
MflI 1077 GGATCC [GA]^GATC[CT]
1488 AGATCT
MluNI 629 TGGCCA TGG^CCA
705 TGGCCA
MscI 629 TGGCCA TGG^CCA
705 TGGCCA
MslI 70 CATGACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
847 CACTATAATG
MspA1I 824 CAGCTG C[AC]G^C[GT]G
1073 CAGCGG
PpuMI 276 GGGTCCT [GA]G^G[AT]CC[CT]
1783 AGGTCCC
PstI 595 CTGCAG CTGCA^G
1492 CTGCAG
SmaI 131 CCCGGG CCC^GGG
973 CCCGGG
TaqI 216 TCGA T^CGA
578 TCGA
TthHB8I 216 TCGA T^CGA
578 TCGA
XhoII 1077 GGATCC [GA]^GATC[CT]
1488 AGATCT
XmaCI 131 CCCGGG C^CCGGG
973 CCCGGG
XmaI 131 CCCGGG C^CCGGG
973 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI AvaII BbsI
BbvI BcnI BmyI BpuAI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HapII HgaI HinfI
HpaII ItaI MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI