Restriction map for
pF1KB6241
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccII AccIII AflII
AgeI AhdI AlwI AlwNI Aor51HI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BcnI BfrI BglII BlnI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspHI BspMI BsrBI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstYI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
HaeII HpaI KasI KpnI KspI
MfeI MflI MluI MluNI MroI
MscI MunI MvnI NarI NciI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 1258 GTCTAC GT^[AC][GT]AC
AclI 1541 AACGTT AA^CGTT
AcyI 227 GACGCC G[GA]^CG[CT]C
AflIII 264 ACATGT A^C[GA][CT]GT
Alw44I 1009 GTGCAC G^TGCAC
ApaI 101 GGGCCC GGGCC^C
ApaLI 1009 GTGCAC G^TGCAC
AspHI 1009 GTGCAC G[AT]GC[AT]^C
AvaI 1698 CTCGGG C^[CT]CG[GA]G
AvaII 4 GGACC G^G[AT]CC
BciVI 2030 GGATAC GTATCC(6/5)
BclI 327 TGATCA T^GATCA
BglI 230 GCCGGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 84 GCTGAGC GC^T[ACGT]AGC
BpmI 124 CTGGAG CTGGAG(16/14)
Bpu1102I 84 GCTGAGC GC^T[ACGT]AGC
BsaAI 2207 TACGTG [CT]AC^GT[GA]
BsaBI 579 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 227 GACGCC G[GA]^CG[CT]C
BsiHKAI 1009 GTGCAC G[AT]GC[AT]^C
BsmFI 3 GGGAC GGGAC(10/14)
BsoBI 1698 CTCGGG C^[CT]CG[GA]G
BspLU11I 264 ACATGT A^CATGT
BsrDI 665 CATTGC GCAATG(2/0)
BsrFI 230 GCCGGC [GA]^CCGG[CT]
BstAPI 1721 GCAAGGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 84 GCTGAGC GC^T[ACGT]AGC
CfoI 937 GCGC GCG^C
Cfr10I 230 GCCGGC [GA]^CCGG[CT]
DsaI 386 CCATGG C^C[GA][CT]GG
EcoRI 155 GAATTC G^AATTC
EcoT14I 386 CCATGG C^C[AT][AT]GG
FauI 1730 GCGGG CCCGC(4/6)
FbaI 327 TGATCA T^GATCA
HgaI 227 GACGC GACGC(5/10)
HhaI 937 GCGC GCG^C
Hin1I 227 GACGCC G[GA]^CG[CT]C
HinP1I 937 GCGC G^CGC
MamI 579 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 230 GCCGGC GCC^GGC
NcoI 386 CCATGG C^CATGG
NgoMIV 230 GCCGGC G^CCGGC
PpuMI 3 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1541 AACGTT AA^CGTT
PstI 1551 CTGCAG CTGCA^G
SfaNI 1046 GATGC GCATC(5/9)
StuI 2131 AGGCCT AGG^CCT
StyI 386 CCATGG C^C[AT][AT]GG
TfiI 282 GAATC G^A[AT]TC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 155 GAATTC [GA]^AATT[CT]
519 AAATTC
AfaI 108 GTAC GT^AC
2225 GTAC
AocI 7 CCTCAGG CC^T[ACGT]AGG
1518 CCTGAGG
ApoI 155 GAATTC [GA]^AATT[CT]
519 AAATTC
BanII 87 GAGCCC G[GA]GC[CT]^C
101 GGGCCC
BbsI 224 GAAGAC GAAGAC(2/6)
1773 GAAGAC
BbvI 1875 GCAGC GCAGC(8/12)
81 GCTGC
BpuAI 224 GAAGAC GAAGAC(2/6)
1773 GAAGAC
Bsp1407I 107 TGTACA T^GTACA
2224 TGTACA
BsrGI 107 TGTACA T^GTACA
2224 TGTACA
Bsu36I 7 CCTCAGG CC^T[ACGT]AGG
1518 CCTGAGG
DpnII 328 GATC ^GATC
1353 GATC
EaeI 232 CGGCCA [CT]^GGCC[GA]
1059 TGGCCG
Eco81I 7 CCTCAGG CC^T[ACGT]AGG
1518 CCTGAGG
HapII 231 CCGG C^CGG
570 CCGG
HindIII 333 AAGCTT A^AGCTT
1233 AAGCTT
HpaII 231 CCGG C^CGG
570 CCGG
MaeII 1542 ACGT A^CGT
2208 ACGT
MboI 328 GATC ^GATC
1353 GATC
MspA1I 350 CAGCTG C[AC]G^C[GT]G
1995 CAGCTG
MspI 231 CCGG C^CGG
570 CCGG
NdeII 328 GATC ^GATC
1353 GATC
PleI 457 GAGTC GAGTC(4/5)
658 GAGTC
PvuII 350 CAGCTG CAG^CTG
1995 CAGCTG
RsaI 108 GTAC GT^AC
2225 GTAC
Sau3AI 328 GATC ^GATC
1353 GATC
SmlI 426 CTCAAG C^T[CT][GA]AG
739 CTCAAG
SspBI 107 TGTACA T^GTACA
2224 TGTACA
TseI 81 GCTGC G^C[AT]GC
1875 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI Asp700I BfaI BmyI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI Cac8I Cfr13I
DdeI DraII EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HincII HindII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboII MnlI MseI MslI
MvaI MwoI NlaIII NlaIV NspI
Sau96I ScrFI SfcI Tru9I Tsp45I
Tsp509I TspRI XmnI