Restriction map for
pF1KB6237
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII AccI AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BbsI BbvCI BbvI BcgI
BciVI BcnI BfrI BlnI BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsmBI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoT22I FauI Fnu4HI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
ItaI KasI KspI MaeII MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TseI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 286 GGTACC G^GTACC
ApaI 1201 GGGCCC GGGCC^C
Asp718I 286 GGTACC G^GTACC
BalI 141 TGGCCA TGG^CCA
BanI 286 GGTACC G^G[CT][GA]CC
BclI 840 TGATCA T^GATCA
BfaI 421 CTAG C^TAG
BglI 1031 GCCAGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 6 AGATCT A^GATCT
BlpI 1636 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1636 GCTAAGC GC^T[ACGT]AGC
BsiWI 1250 CGTACG C^GTACG
BspHI 1680 TCATGA T^CATGA
BssSI 1488 CACGAG CACGAG(-5/-1)
BstEII 495 GGTAACC G^GT[ACGT]ACC
BstPI 495 GGTAACC G^GT[ACGT]ACC
CelII 1636 GCTAAGC GC^T[ACGT]AGC
DraI 1084 TTTAAA TTT^AAA
DsaI 256 CCATGG C^C[GA][CT]GG
EaeI 141 TGGCCA [CT]^GGCC[GA]
EcoNI 1109 CCTTCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 495 GGTAACC G^GT[ACGT]ACC
EcoRV 2207 GATATC GAT^ATC
FbaI 840 TGATCA T^GATCA
KpnI 286 GGTACC GGTAC^C
MaeI 421 CTAG C^TAG
MluNI 141 TGGCCA TGG^CCA
MscI 141 TGGCCA TGG^CCA
MspA1I 130 CAGCTG C[AC]G^C[GT]G
MwoI 1031 GCCAGAGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 256 CCATGG C^CATGG
NdeI 1225 CATATG CA^TATG
PflMI 1041 CCACTTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 952 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 130 CAGCTG CAG^CTG
RcaI 1680 TCATGA T^CATGA
SacI 1959 GAGCTC GAGCT^C
SapI 355 GAAGAGC GCTCTTC(1/4)
SexAI 188 ACCTGGT A^CC[AT]GGT
SfcI 197 CTATAG C^T[GA][CT]AG
SmlI 1574 CTCAAG C^T[CT][GA]AG
TaqI 1869 TCGA T^CGA
TthHB8I 1869 TCGA T^CGA
Van91I 1041 CCACTTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 287 GTAC GT^AC
1251 GTAC
AlwI 1734 GATCC GGATC(4/5)
260 GGATC
BanII 1201 GGGCCC G[GA]GC[CT]^C
1959 GAGCTC
BsaI 604 GAGACC GGTCTC(1/5)
192 GGTCTC
BsgI 293 GTGCAG GTGCAG(16/14)
1343 GTGCAG
BsmFI 42 GGGAC GGGAC(10/14)
247 GGGAC
BstYI 6 AGATCT [GA]^GATC[CT]
1733 AGATCC
Cac8I 459 GCAGGC GC[ACGT]^[ACGT]GC
1283 GCCAGC
EarI 355 GAAGAG CTCTTC(1/4)
601 GAAGAG
EcoT14I 36 CCAAGG C^C[AT][AT]GG
256 CCATGG
HapII 780 CCGG C^CGG
2040 CCGG
HindIII 1583 AAGCTT A^AGCTT
1610 AAGCTT
HpaII 780 CCGG C^CGG
2040 CCGG
Ksp632I 355 GAAGAG CTCTTC(1/4)
601 GAAGAG
MflI 6 AGATCT [GA]^GATC[CT]
1733 AGATCC
MspI 780 CCGG C^CGG
2040 CCGG
PleI 22 GACTC GAGTC(4/5)
203 GACTC
RsaI 287 GTAC GT^AC
1251 GTAC
StuI 1190 AGGCCT AGG^CCT
1442 AGGCCT
StyI 36 CCAAGG C^C[AT][AT]GG
256 CCATGG
XhoII 6 AGATCT [GA]^GATC[CT]
1733 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AcsI AluI AlwNI ApoI Asp700I
AspHI AvaII BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII DraII
EcoO109I EcoRI EcoRII FokI HaeIII
HinfI HphI MaeIII MboI MboII
MnlI MseI MslI MvaI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TfiI Tru9I Tsp45I Tsp509I
TspRI XmnI