Restriction map for
pF1KB6235
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# NO CUT ENZYMES ( 116 enzymes) #
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Acc65I AccII AccIII AclI AflII
AgeI Alw44I AocI ApaI ApaLI
AscI Asp700I Asp718I AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BclI BcnI
BfrI BglI BglII BlnI BsaAI
BsaWI BseAI BseRI BsiEI BsiWI
BsmI BspDI BspEI BspHI BsrDI
BsrFI BssHII Bst1107I BstEII BstPI
BstUI BstYI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraII DrdI EaeI
EagI EclXI Eco52I Eco81I EcoO109I
EcoO65I FbaI FseI FspI HapII
HindIII HpaI HpaII KasI KpnI
KspI MflI MluI MluNI MroI
MscI MspI MvnI NaeI NarI
NciI NdeI NgoMIV NotI NruI
PacI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SapI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
XbaI XcmI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 1336 GACGTC GACGT^C
AhdI 508 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1875 GATCC GGATC(4/5)
Aor51HI 2218 AGCGCT AGC^GCT
AseI 681 ATTAAT AT^TAAT
AsnI 681 ATTAAT AT^TAAT
AspEI 508 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 651 GGACC G^G[AT]CC
BciVI 228 GTATCC GTATCC(6/5)
BlpI 2094 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2094 GCTGAGC GC^T[ACGT]AGC
BsaBI 1323 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 318 GGTCTC GGTCTC(1/5)
BsmBI 490 GAGACG CGTCTC(1/5)
BsmFI 1829 GGGAC GGGAC(10/14)
BspLU11I 150 ACATGT A^CATGT
BsrBI 161 CCGCTC CCGCTC(-3/-3)
BssSI 1526 CTCGTG CACGAG(-5/-1)
BstBI 1376 TTCGAA TT^CGAA
CelII 2094 GCTGAGC GC^T[ACGT]AGC
DraI 1977 TTTAAA TTT^AAA
DraIII 436 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 508 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2218 AGCGCT AGC^GCT
EcoNI 1512 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 426 GAATTC G^AATTC
EcoRV 1045 GATATC GAT^ATC
EcoT22I 974 ATGCAT ATGCA^T
FauI 160 CCCGC CCCGC(4/6)
MamI 1323 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 473 CAATTG C^AATTG
MslI 748 CACTACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1460 CCGCTG C[AC]G^C[GT]G
MunI 473 CAATTG C^AATTG
NcoI 1296 CCATGG C^CATGG
NheI 1852 GCTAGC G^CTAGC
NsiI 974 ATGCAT ATGCA^T
NspV 1376 TTCGAA TT^CGAA
PshBI 681 ATTAAT AT^TAAT
ScaI 2194 AGTACT AGT^ACT
SfuI 1376 TTCGAA TT^CGAA
SphI 2120 GCATGC GCATG^C
SwaI 1976 ATTTAAAT ATTT^AAAT
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcsI 426 GAATTC [GA]^AATT[CT]
1380 AAATTT
AcyI 361 GGCGTC G[GA]^CG[CT]C
1336 GACGTC
AflIII 150 ACATGT A^C[GA][CT]GT
1496 ACACGT
ApoI 426 GAATTC [GA]^AATT[CT]
1380 AAATTT
BbsI 799 GAAGAC GAAGAC(2/6)
1757 GAAGAC
BpuAI 799 GAAGAC GAAGAC(2/6)
1757 GAAGAC
BsaHI 361 GGCGTC G[GA]^CG[CT]C
1336 GACGTC
Bsp1407I 251 TGTACA T^GTACA
611 TGTACA
BsrGI 251 TGTACA T^GTACA
611 TGTACA
BstAPI 1522 GCAGCTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1785 GCAGTTTCTGC
CfoI 1152 GCGC GCG^C
2219 GCGC
DsaI 314 CCGTGG C^C[GA][CT]GG
1296 CCATGG
EcoT14I 1296 CCATGG C^C[AT][AT]GG
1451 CCAAGG
HaeII 1151 GGCGCT [GA]GCGC^[CT]
2218 AGCGCT
HaeIII 459 GGCC GG^CC
1515 GGCC
HgaI 492 GACGC GACGC(5/10)
362 GCGTC
HhaI 1152 GCGC GCG^C
2219 GCGC
Hin1I 361 GGCGTC G[GA]^CG[CT]C
1336 GACGTC
HinP1I 1152 GCGC G^CGC
2219 GCGC
PaeR7I 980 CTCGAG C^TCGAG
2134 CTCGAG
PstI 1057 CTGCAG CTGCA^G
2240 CTGCAG
SalI 1223 GTCGAC G^TCGAC
1585 GTCGAC
SspBI 251 TGTACA T^GTACA
611 TGTACA
SspI 1089 AATATT AAT^ATT
1426 AATATT
StyI 1296 CCATGG C^C[AT][AT]GG
1451 CCAAGG
XhoI 980 CTCGAG C^TCGAG
2134 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccI AciI AfaI AluI AlwNI
AspHI AspI AvaI BbvI BfaI
BmyI BpmI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI Cac8I Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I