Restriction map for
pF1KB6837
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# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII Acc65I AccI AccII AciI
AclI AfaI AflII AflIII AgeI
AhdI AluI Alw44I AlwI AlwNI
AocI Aor51HI ApaLI AscI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BcgI BciVI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BseRI
BsgI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HhaI HinP1I HindIII HinfI KasI
KpnI KspI MaeI MaeII MamI
MfeI MluI MluNI MscI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI PvuII RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI TfiI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 24 TCCGGA T^CCGGA
AcsI 271 AAATTT [GA]^AATT[CT]
AcyI 75 GACGCC G[GA]^CG[CT]C
ApaI 32 GGGCCC GGGCC^C
ApoI 271 AAATTT [GA]^AATT[CT]
AseI 301 ATTAAT AT^TAAT
AsnI 301 ATTAAT AT^TAAT
AspHI 404 GAGCAC G[AT]GC[AT]^C
BanII 32 GGGCCC G[GA]GC[CT]^C
BbsI 133 GAAGAC GAAGAC(2/6)
BbvCI 316 GCTGAGG CCTCAGC(-5/-2)
BcnI 65 CCGGG CC^[GC]GG
BglII 186 AGATCT A^GATCT
BpuAI 133 GAAGAC GAAGAC(2/6)
BsaHI 75 GACGCC G[GA]^CG[CT]C
BsaWI 24 TCCGGA [AT]^CCGG[AT]
BseAI 24 TCCGGA T^CCGGA
BsiHKAI 404 GAGCAC G[AT]GC[AT]^C
BsmAI 21 GTCTC GTCTC(1/5)
BsmBI 20 CGTCTC CGTCTC(1/5)
BsmFI 7 GTCCC GGGAC(10/14)
BspEI 24 TCCGGA T^CCGGA
BspHI 375 TCATGA T^CATGA
BstF5I 512 GGATG GGATG(2/0)
BstYI 186 AGATCT [GA]^GATC[CT]
DdeI 317 CTGAG C^T[ACGT]AG
DraI 274 TTTAAA TTT^AAA
EaeI 43 CGGCCA [CT]^GGCC[GA]
FokI 512 GGATG GGATG(9/13)
HgaI 75 GACGC GACGC(5/10)
Hin1I 75 GACGCC G[GA]^CG[CT]C
HincII 489 GTTAAC GT[CT]^[GA]AC
HindII 489 GTTAAC GT[CT]^[GA]AC
HpaI 489 GTTAAC GTT^AAC
HphI 345 GGTGA GGTGA(8/7)
MaeIII 89 GTGAC ^GT[ACGT]AC
MflI 186 AGATCT [GA]^GATC[CT]
MroI 24 TCCGGA T^CCGGA
MslI 328 CACTATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 65 CCGGG CC^[GC]GG
PshBI 301 ATTAAT AT^TAAT
RcaI 375 TCATGA T^CATGA
Tsp45I 89 GTGAC ^GT[GC]AC
Tsp509I 272 AATT ^AATT
XhoII 186 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BbvI 417 GCAGC GCAGC(8/12)
453 GCAGC
BmyI 32 GGGCCC G[AGT]GC[ACT]^C
404 GAGCAC
Bsp1286I 32 GGGCCC G[AGT]GC[ACT]^C
404 GAGCAC
BsrI 153 ACTGG ACTGG(1/-1)
252 ACTGG
DpnII 187 GATC ^GATC
197 GATC
DraII 31 GGGGCCC [GA]G^G[ACGT]CC[CT]
32 GGGCCCT
EarI 423 GAAGAG CTCTTC(1/4)
436 GAAGAG
EcoO109I 31 GGGGCCC [GA]G^G[ACGT]CC[CT]
32 GGGCCCT
Fnu4HI 417 GCAGC GC^[ACGT]GC
453 GCAGC
HapII 25 CCGG C^CGG
65 CCGG
HpaII 25 CCGG C^CGG
65 CCGG
ItaI 417 GCAGC GC^[ACGT]GC
453 GCAGC
Ksp632I 423 GAAGAG CTCTTC(1/4)
436 GAAGAG
MboI 187 GATC ^GATC
197 GATC
MspI 25 CCGG C^CGG
65 CCGG
NdeII 187 GATC ^GATC
197 GATC
NlaIII 334 CATG CATG^
376 CATG
PstI 190 CTGCAG CTGCA^G
415 CTGCAG
Sau3AI 187 GATC ^GATC
197 GATC
SfaNI 363 GATGC GCATC(5/9)
369 GATGC
TseI 417 GCAGC G^C[AT]GC
453 GCAGC
TspRI 143 CAGTG CA[GC]TG[ACGT][ACGT]^
251 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
BpmI BsaJI BsiYI BslI BssKI
BstNI Cfr13I EcoRII HaeIII MboII
MnlI MseI MvaI MwoI NlaIV
Sau96I ScrFI SfcI Tru9I