##################################
# NO CUT ENZYMES (  197 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BciVI       BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstF5I      BstXI       BstYI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        ClaI        CpoI        
DraI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoRI       EcoRV       EcoT14I     
EcoT22I     FokI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        KasI        
KpnI        Ksp632I     MamI        MboII       MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MunI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SalI        SapI        SbfI        
ScaI        SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TfiI        
TseI        Tsp509I     Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccII         313  CGCG                 CG^CG
AvaII           9  GGACC                G^G[AT]CC
BclI          129  TGATCA               T^GATCA
BfaI          164  CTAG                 C^TAG
BpmI          352  CTGGAG               CTGGAG(16/14)
BsmAI         107  GTCTC                GTCTC(1/5)
BsmBI         106  CGTCTC               CGTCTC(1/5)
BsrFI         239  ACCGGC               [GA]^CCGG[CT]
BsrI          255  ACTGG                ACTGG(1/-1)
BstUI         313  CGCG                 CG^CG
Cfr10I        239  ACCGGC               [GA]^CCGG[CT]
DdeI          113  CTCAG                C^T[ACGT]AG
DpnII         130  GATC                 ^GATC
DraII          65  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DsaI          312  CCGCGG               C^C[GA][CT]GG
EcoO109I       65  AGGCCCT              [GA]G^G[ACGT]CC[CT]
FauI           89  CCCGC                CCCGC(4/6)
FbaI          129  TGATCA               T^GATCA
Fnu4HI        314  GCGGC                GC^[ACGT]GC
HaeIII         66  GGCC                 GG^CC
HapII         240  CCGG                 C^CGG
HpaII         240  CCGG                 C^CGG
ItaI          314  GCGGC                GC^[ACGT]GC
KspI          312  CCGCGG               CCGC^GG
MaeI          164  CTAG                 C^TAG
MaeII         105  ACGT                 A^CGT
MboI          130  GATC                 ^GATC
MseI          248  TTAA                 T^TAA
MspA1I        312  CCGCGG               C[AC]G^C[GT]G
MspI          240  CCGG                 C^CGG
MvnI          313  CGCG                 CG^CG
MwoI          167  GCACATTCCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII         130  GATC                 ^GATC
NspI          243  GCATGT               [GA]CATG^[CT]
SacII         312  CCGCGG               CCGC^GG
Sau3AI        130  GATC                 ^GATC
SexAI         371  ACCAGGT              A^CC[AT]GGT
Tru9I         248  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AluI          344  AGCT                 AG^CT
              356  AGCT                
BsmFI           8  GGGAC                GGGAC(10/14)
              338  GGGAC               
BstEII        258  GGTCACC              G^GT[ACGT]ACC
              367  GGTGACC             
BstPI         258  GGTCACC              G^GT[ACGT]ACC
              367  GGTGACC             
Cfr13I          9  GGACC                G^G[ACGT]CC
               66  GGCCC               
EcoO65I       258  GGTCACC              G^GT[ACGT]ACC
              367  GGTGACC             
MaeIII        259  GTCAC                ^GT[ACGT]AC
              368  GTGAC               
MnlI           52  GAGG                 CCTC(7/6)
              334  GAGG                
NlaIII         60  CATG                 CATG^
              244  CATG                
NlaIV           8  GGGACC               GG[ACGT]^[ACGT]CC
               19  GGAACC              
Sau96I          9  GGACC                G^G[ACGT]CC
               66  GGCCC               
Tsp45I        259  GTCAC                ^GT[GC]AC
              368  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AciI        AfaI        BsaJI       BssKI       BstNI       
EcoRII      HphI        MvaI        RsaI        ScrFI       
TspRI