Restriction map for
pF1KB6202
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BseAI BsgI BsiEI BsiWI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII Bst1107I BstBI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoRV EcoT14I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MluI MroI MseI
MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI StyI
SwaI Tru9I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 254 CGCG CG^CG
AfaI 153 GTAC GT^AC
Alw44I 1764 GTGCAC G^TGCAC
AlwI 61 GGATC GGATC(4/5)
AocI 31 CCTCAGG CC^T[ACGT]AGG
ApaLI 1764 GTGCAC G^TGCAC
BalI 783 TGGCCA TGG^CCA
BanII 172 GAGCTC G[GA]GC[CT]^C
BcnI 156 CCGGG CC^[GC]GG
BsaBI 1471 GATATACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1660 ACCGGA [AT]^CCGG[AT]
BspMI 1793 GCAGGT ACCTGC(4/8)
BsrDI 650 GCAATG GCAATG(2/0)
BssSI 549 CACGAG CACGAG(-5/-1)
BstAPI 1598 GCAGTGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 760 GGTAACC G^GT[ACGT]ACC
BstPI 760 GGTAACC G^GT[ACGT]ACC
BstUI 254 CGCG CG^CG
BstYI 61 GGATCT [GA]^GATC[CT]
Bsu36I 31 CCTCAGG CC^T[ACGT]AGG
DsaI 98 CCGTGG C^C[GA][CT]GG
EaeI 783 TGGCCA [CT]^GGCC[GA]
Eco81I 31 CCTCAGG CC^T[ACGT]AGG
EcoO65I 760 GGTAACC G^GT[ACGT]ACC
HincII 1293 GTCAAC GT[CT]^[GA]AC
HindII 1293 GTCAAC GT[CT]^[GA]AC
HindIII 341 AAGCTT A^AGCTT
MamI 1471 GATATACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 513 CAATTG C^AATTG
MflI 61 GGATCT [GA]^GATC[CT]
MluNI 783 TGGCCA TGG^CCA
MscI 783 TGGCCA TGG^CCA
MunI 513 CAATTG C^AATTG
MvnI 254 CGCG CG^CG
MwoI 1598 GCAGTGAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 156 CCGGG CC^[GC]GG
PleI 468 GAGTC GAGTC(4/5)
PstI 720 CTGCAG CTGCA^G
RsaI 153 GTAC GT^AC
SacI 172 GAGCTC GAGCT^C
SapI 707 GCTCTTC GCTCTTC(1/4)
SspI 730 AATATT AAT^ATT
XhoII 61 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AspHI 172 GAGCTC G[AT]GC[AT]^C
1764 GTGCAC
BbvI 123 GCAGC GCAGC(8/12)
722 GCAGC
BmyI 172 GAGCTC G[AGT]GC[ACT]^C
1764 GTGCAC
BpmI 169 CTGGAG CTGGAG(16/14)
1577 CTGGAG
BseRI 115 GAGGAG GAGGAG(10/8)
262 GAGGAG
BsiHKAI 172 GAGCTC G[AT]GC[AT]^C
1764 GTGCAC
BsiYI 150 CCTGTACCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
318 CCCACAAAAGG
BslI 150 CCTGTACCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
318 CCCACAAAAGG
Bsp1286I 172 GAGCTC G[AGT]GC[ACT]^C
1764 GTGCAC
BstNI 39 CCTGG CC^[AT]GG
532 CCTGG
EcoRII 39 CCTGG ^CC[AT]GG
532 CCTGG
EcoT22I 1256 ATGCAT ATGCA^T
1642 ATGCAT
FauI 1355 GCGGG CCCGC(4/6)
1607 GCGGG
HapII 156 CCGG C^CGG
1661 CCGG
HpaII 156 CCGG C^CGG
1661 CCGG
MaeII 92 ACGT A^CGT
674 ACGT
MspI 156 CCGG C^CGG
1661 CCGG
MvaI 39 CCTGG CC^[AT]GG
532 CCTGG
NsiI 1256 ATGCAT ATGCA^T
1642 ATGCAT
NspI 1258 GCATGC [GA]CATG^[CT]
1640 GCATGC
PpuMI 35 AGGTCCT [GA]G^G[AT]CC[CT]
527 AGGACCC
SfcI 177 CTATAG C^T[GA][CT]AG
720 CTGCAG
SmlI 799 CTCAAG C^T[CT][GA]AG
1972 CTCAAG
SphI 1258 GCATGC GCATG^C
1640 GCATGC
TaqI 1349 TCGA T^CGA
1482 TCGA
TfiI 543 GATTC G^A[AT]TC
1316 GAATC
TseI 123 GCAGC G^C[AT]GC
722 GCAGC
TthHB8I 1349 TCGA T^CGA
1482 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AcsI AluI AlwNI ApoI
Asp700I AvaII BfaI BsaJI BsmAI
BsmI BsrI BssKI BstF5I BstXI
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRI Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MslI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI StuI Tsp45I
Tsp509I TspRI XmnI