Restriction map for
pF1KB6175
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BbeI BcgI
BciVI BclI BfrI BglII BlnI
BsaBI BsaI BsaWI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DrdI Eam1105I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HpaI
KasI KpnI MaeIII MamI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuII RcaI RsrII
SalI SapI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SspBI
SspI SwaI TfiI Tsp45I Tsp509I
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AfaI 780 GTAC GT^AC
AflIII 1769 ACGTGT A^C[GA][CT]GT
AocI 1447 CCTGAGG CC^T[ACGT]AGG
BamHI 46 GGATCC G^GATCC
BbrPI 1768 CACGTG CAC^GTG
BfaI 1399 CTAG C^TAG
BlpI 519 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 519 GCTCAGC GC^T[ACGT]AGC
BsmBI 1693 GAGACG CGTCTC(1/5)
BsrDI 446 GCAATG GCAATG(2/0)
BssSI 1549 CACGAG CACGAG(-5/-1)
BstF5I 260 CATCC GGATG(2/0)
BstYI 46 GGATCC [GA]^GATC[CT]
Bsu36I 1447 CCTGAGG CC^T[ACGT]AGG
CelII 519 GCTCAGC GC^T[ACGT]AGC
Eco81I 1447 CCTGAGG CC^T[ACGT]AGG
EcoNI 1590 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1043 CCATGG C^C[AT][AT]GG
FokI 260 CATCC GGATG(9/13)
HincII 859 GTCAAC GT[CT]^[GA]AC
HindII 859 GTCAAC GT[CT]^[GA]AC
HindIII 655 AAGCTT A^AGCTT
KspI 1352 CCGCGG CCGC^GG
MaeI 1399 CTAG C^TAG
MflI 46 GGATCC [GA]^GATC[CT]
MseI 355 TTAA T^TAA
NcoI 1043 CCATGG C^CATGG
NruI 1410 TCGCGA TCG^CGA
NspI 236 GCATGC [GA]CATG^[CT]
PmaCI 1768 CACGTG CAC^GTG
PmlI 1768 CACGTG CAC^GTG
PvuI 635 CGATCG CGAT^CG
RsaI 780 GTAC GT^AC
SacII 1352 CCGCGG CCGC^GG
SbfI 1785 CCTGCAGG CCTGCA^GG
ScaI 779 AGTACT AGT^ACT
SmlI 529 CTCAAG C^T[CT][GA]AG
SphI 236 GCATGC GCATG^C
Sse8387I 1785 CCTGCAGG CCTGCA^GG
StyI 1043 CCATGG C^C[AT][AT]GG
Tru9I 355 TTAA T^TAA
XcmI 1796 CCACAGCCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 46 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 47 GATCC GGATC(4/5)
46 GGATC
Aor51HI 533 AGCGCT AGC^GCT
974 AGCGCT
AviII 596 TGCGCA TGC^GCA
1259 TGCGCA
BalI 758 TGGCCA TGG^CCA
1776 TGGCCA
BbsI 207 GAAGAC GAAGAC(2/6)
1233 GAAGAC
BpuAI 207 GAAGAC GAAGAC(2/6)
1233 GAAGAC
BsaAI 1441 TACGTG [CT]AC^GT[GA]
1768 CACGTG
BsmAI 1693 GAGAC GTCTC(1/5)
374 GTCTC
BstXI 1767 CCACGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1796 CCACAGCCGTGG
DraIII 76 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1765 CACCACGTG
EarI 1180 GAAGAG CTCTTC(1/4)
1471 GAAGAG
Eco47III 533 AGCGCT AGC^GCT
974 AGCGCT
FspI 596 TGCGCA TGC^GCA
1259 TGCGCA
Ksp632I 1180 GAAGAG CTCTTC(1/4)
1471 GAAGAG
MluNI 758 TGGCCA TGG^CCA
1776 TGGCCA
MscI 758 TGGCCA TGG^CCA
1776 TGGCCA
SmaI 125 CCCGGG CCC^GGG
1085 CCCGGG
StuI 173 AGGCCT AGG^CCT
1610 AGGCCT
XmaCI 125 CCCGGG C^CCGGG
1085 CCCGGG
XmaI 125 CCCGGG C^CCGGG
1085 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcyI AluI Alw44I
AlwNI ApaI ApaLI AspHI AvaI
AvaII BanI BanII BbvCI BbvI
BcnI BglI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PpuMI PstI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TseI TspRI TthHB8I
Van91I