Restriction map for
pF1KB6153
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AgeI AhdI Aor51HI ApaI AscI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BbeI BcgI
BciVI BclI BlnI BlpI Bpu1102I
BsaHI BsaI BsaWI BseAI BsiWI
BsmBI BsoBI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII Cfr10I Cfr13I CpoI
DraII DraIII DsaI Eam1105I Eco47III
EcoO109I EcoO65I EcoRV FauI FbaI
FseI FspI HaeII HgaI Hin1I
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI Sau96I SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
SwaI Tth111I Van91I XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AccI 1682 GTAGAC GT^[AC][GT]AC
AccII 499 CGCG CG^CG
AflII 649 CTTAAG C^TTAAG
AflIII 716 ACGTGT A^C[GA][CT]GT
Alw44I 1288 GTGCAC G^TGCAC
AocI 1152 CCTGAGG CC^T[ACGT]AGG
ApaLI 1288 GTGCAC G^TGCAC
AseI 184 ATTAAT AT^TAAT
AsnI 184 ATTAAT AT^TAAT
AspHI 1288 GTGCAC G[AT]GC[AT]^C
BalI 1782 TGGCCA TGG^CCA
BamHI 1621 GGATCC G^GATCC
BanII 1251 GAGCCC G[GA]GC[CT]^C
BbrPI 715 CACGTG CAC^GTG
BbvCI 296 CCTCAGC CCTCAGC(-5/-2)
BfaI 118 CTAG C^TAG
BfrI 649 CTTAAG C^TTAAG
BglI 394 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 55 CTGGAG CTGGAG(16/14)
BsaAI 715 CACGTG [CT]AC^GT[GA]
BsaBI 1630 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 916 GAGGAG GAGGAG(10/8)
BsgI 704 GTGCAG GTGCAG(16/14)
BsiEI 906 CGGCCG CG[GA][CT]^CG
BsiHKAI 1288 GTGCAC G[AT]GC[AT]^C
BsmAI 1199 GAGAC GTCTC(1/5)
BsmFI 1207 GGGAC GGGAC(10/14)
BsmI 1105 GAATGC GAATGC(1/-1)
Bsp1407I 264 TGTACA T^GTACA
BspDI 461 ATCGAT AT^CGAT
BspMI 1230 GCAGGT ACCTGC(4/8)
BsrGI 264 TGTACA T^GTACA
BstUI 499 CGCG CG^CG
Bsu36I 1152 CCTGAGG CC^T[ACGT]AGG
ClaI 461 ATCGAT AT^CGAT
DraI 1489 TTTAAA TTT^AAA
DrdI 68 GACTGTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 906 CGGCCG C^GGCCG
EclXI 906 CGGCCG C^GGCCG
Eco52I 906 CGGCCG C^GGCCG
Eco81I 1152 CCTGAGG CC^T[ACGT]AGG
EcoNI 144 CCTTTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 255 GAATTC G^AATTC
EcoT14I 1677 CCTTGG C^C[AT][AT]GG
HincII 1522 GTTGAC GT[CT]^[GA]AC
HindII 1522 GTTGAC GT[CT]^[GA]AC
HindIII 1093 AAGCTT A^AGCTT
HphI 989 TCACC GGTGA(8/7)
MaeI 118 CTAG C^TAG
MaeII 716 ACGT A^CGT
MamI 1630 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1782 TGGCCA TGG^CCA
MscI 1782 TGGCCA TGG^CCA
MvnI 499 CGCG CG^CG
PmaCI 715 CACGTG CAC^GTG
PmlI 715 CACGTG CAC^GTG
PshBI 184 ATTAAT AT^TAAT
PstI 1228 CTGCAG CTGCA^G
SapI 865 GAAGAGC GCTCTTC(1/4)
SfcI 1228 CTGCAG C^T[GA][CT]AG
SmlI 649 CTTAAG C^T[CT][GA]AG
SphI 564 GCATGC GCATG^C
SspBI 264 TGTACA T^GTACA
SspI 1592 AATATT AAT^ATT
StyI 1677 CCTTGG C^C[AT][AT]GG
XbaI 117 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 15 enzymes) #
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AciI 1625 CCGC CCGC(-3/-1)
82 GCGG
BanI 1348 GGTGCC G^G[CT][GA]CC
1408 GGTGCC
BcnI 39 CCCGG CC^[GC]GG
270 CCGGG
BglII 759 AGATCT A^GATCT
1703 AGATCT
EaeI 906 CGGCCG [CT]^GGCC[GA]
1782 TGGCCA
EcoT22I 562 ATGCAT ATGCA^T
1771 ATGCAT
MslI 799 CACACAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1260 CATAGCCATG
MspA1I 52 CAGCTG C[AC]G^C[GT]G
1219 CAGCTG
NciI 39 CCCGG CC^[GC]GG
270 CCGGG
NsiI 562 ATGCAT ATGCA^T
1771 ATGCAT
NspI 564 GCATGC [GA]CATG^[CT]
1764 ACATGC
PvuII 52 CAGCTG CAG^CTG
1219 CAGCTG
SfaNI 561 GATGC GCATC(5/9)
1773 GCATC
Tsp45I 720 GTCAC ^GT[GC]AC
855 GTCAC
XcmI 443 CCAAATGGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
608 CCAGTATGGATGTGG
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
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AcsI AfaI AluI AlwI AlwNI
ApoI BbsI BbvI BmyI BpuAI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI ScrFI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII