Restriction map for
pF1KB6143
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI Alw44I AocI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspI AvrII BamHI BbeI BbrPI
BciVI BclI BcnI BfrI BlnI
BsaAI BsaBI BsaHI BsaI BseRI
BsiEI BsiWI BsmI Bsp1407I BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I Cfr10I CpoI DraI DraIII
EagI EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MslI MunI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 1638 CGCG CG^CG
AccIII 96 TCCGGA T^CCGGA
AfaI 1707 GTAC GT^AC
AflIII 1637 ACGCGT A^C[GA][CT]GT
AhdI 316 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1274 AGCGCT AGC^GCT
AspEI 316 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1264 GAGCAC G[AT]GC[AT]^C
AvaI 14 CTCGGG C^[CT]CG[GA]G
AvaII 466 GGACC G^G[AT]CC
AviII 977 TGCGCA TGC^GCA
BanI 1158 GGCACC G^G[CT][GA]CC
BbsI 499 GAAGAC GAAGAC(2/6)
BcgI 1734 CGAAACGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 4 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 565 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 565 GCTCAGC GC^T[ACGT]AGC
BpuAI 499 GAAGAC GAAGAC(2/6)
BseAI 96 TCCGGA T^CCGGA
BsgI 1278 CTGCAC GTGCAG(16/14)
BsiHKAI 1264 GAGCAC G[AT]GC[AT]^C
BsmBI 886 CGTCTC CGTCTC(1/5)
BsmFI 431 GGGAC GGGAC(10/14)
BsoBI 14 CTCGGG C^[CT]CG[GA]G
BspDI 839 ATCGAT AT^CGAT
BspEI 96 TCCGGA T^CCGGA
BspHI 1130 TCATGA T^CATGA
BsrDI 311 GCAATG GCAATG(2/0)
BstAPI 1716 GCAGCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1638 CGCG CG^CG
CelII 565 GCTCAGC GC^T[ACGT]AGC
ClaI 839 ATCGAT AT^CGAT
DrdI 934 GACAAACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 316 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1274 AGCGCT AGC^GCT
FauI 1123 GCGGG CCCGC(4/6)
FspI 977 TGCGCA TGC^GCA
HgaI 885 GCGTC GACGC(5/10)
MaeII 167 ACGT A^CGT
MluI 1637 ACGCGT A^CGCGT
MroI 96 TCCGGA T^CCGGA
MspA1I 548 CAGCTG C[AC]G^C[GT]G
MvnI 1638 CGCG CG^CG
PvuII 548 CAGCTG CAG^CTG
RcaI 1130 TCATGA T^CATGA
RsaI 1707 GTAC GT^AC
SexAI 296 ACCAGGT A^CC[AT]GGT
SpeI 1566 ACTAGT A^CTAGT
XcmI 297 CCAGGTTGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwI 766 GATCC GGATC(4/5)
1676 GGATC
AlwNI 218 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
470 CAGCAACTG
BbvCI 1764 CCTCAGC CCTCAGC(-5/-2)
1752 GCTGAGG
BglII 1374 AGATCT A^GATCT
1452 AGATCT
BsaWI 96 TCCGGA [AT]^CCGG[AT]
1673 ACCGGA
BssSI 1480 CACGAG CACGAG(-5/-1)
1429 CTCGTG
BstXI 1217 CCAAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1424 CCAGTCTCGTGG
DraII 376 GGGGCCC [GA]G^G[ACGT]CC[CT]
1760 AGGCCCT
DsaI 1145 CCATGG C^C[GA][CT]GG
1223 CCATGG
EcoO109I 376 GGGGCCC [GA]G^G[ACGT]CC[CT]
1760 AGGCCCT
HaeII 562 GGCGCT [GA]GCGC^[CT]
1274 AGCGCT
HapII 97 CCGG C^CGG
1674 CCGG
HpaII 97 CCGG C^CGG
1674 CCGG
MspI 97 CCGG C^CGG
1674 CCGG
NcoI 1145 CCATGG C^CATGG
1223 CCATGG
PflMI 422 CCAACAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
469 CCAGCAACTGG
TaqI 809 TCGA T^CGA
840 TCGA
Tsp45I 943 GTCAC ^GT[GC]AC
1559 GTGAC
TthHB8I 809 TCGA T^CGA
840 TCGA
Van91I 422 CCAACAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
469 CCAGCAACTGG
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcsI AluI ApaI ApoI
BalI BanII BbvI BfaI BmyI
BpmI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI StuI StyI
TfiI Tru9I TseI Tsp509I TspRI
XhoII