Restriction map for
pF1KA1930
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# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
AhdI AocI AscI AseI AsnI
Asp700I AspEI AviII BamHI BbrPI
BcgI BciVI BfrI BsaBI BsiWI
BspHI BspLU11I BsrDI BsrFI BssHII
Bst1107I BstBI BstZ17I Bsu36I Cfr10I
CpoI DraI Eam1105I Eco81I EcoT22I
FseI FspI HindIII HpaI KspI
MamI MfeI MluI MunI NaeI
NdeI NgoMIV NheI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 916 GGTACC G^GTACC
AccI 2238 GTCTAC GT^[AC][GT]AC
AccIII 3350 TCCGGA T^CCGGA
Asp718I 916 GGTACC G^GTACC
AspI 2483 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 3252 GGCGCC GGCGC^C
BclI 3219 TGATCA T^GATCA
BglII 674 AGATCT A^GATCT
BsaAI 3166 TACGTG [CT]AC^GT[GA]
BseAI 3350 TCCGGA T^CCGGA
BsiEI 19 CGGCCG CG[GA][CT]^CG
BsmBI 451 CGTCTC CGTCTC(1/5)
Bsp1407I 230 TGTACA T^GTACA
BspDI 415 ATCGAT AT^CGAT
BspEI 3350 TCCGGA T^CCGGA
BspMI 738 GCAGGT ACCTGC(4/8)
BsrGI 230 TGTACA T^GTACA
BssSI 88 CACGAG CACGAG(-5/-1)
BstAPI 3063 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2451 GGTGACC G^GT[ACGT]ACC
BstPI 2451 GGTGACC G^GT[ACGT]ACC
ClaI 415 ATCGAT AT^CGAT
EagI 19 CGGCCG C^GGCCG
EarI 3565 GAAGAG CTCTTC(1/4)
EclXI 19 CGGCCG C^GGCCG
Eco52I 19 CGGCCG C^GGCCG
EcoO65I 2451 GGTGACC G^GT[ACGT]ACC
EcoRI 324 GAATTC G^AATTC
EcoRV 901 GATATC GAT^ATC
FbaI 3219 TGATCA T^GATCA
HincII 991 GTCAAC GT[CT]^[GA]AC
HindII 991 GTCAAC GT[CT]^[GA]AC
KasI 3252 GGCGCC G^GCGCC
KpnI 916 GGTACC GGTAC^C
Ksp632I 3565 GAAGAG CTCTTC(1/4)
MroI 3350 TCCGGA T^CCGGA
MseI 797 TTAA T^TAA
NarI 3252 GGCGCC GG^CGCC
NotI 18 GCGGCCGC GC^GGCCGC
NruI 552 TCGCGA TCG^CGA
PaeR7I 3434 CTCGAG C^TCGAG
PflFI 2483 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2325 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 2805 AGTACT AGT^ACT
SexAI 224 ACCTGGT A^CC[AT]GGT
SfoI 3252 GGCGCC GGC^GCC
SmlI 3434 CTCGAG C^T[CT][GA]AG
SspBI 230 TGTACA T^GTACA
Tru9I 797 TTAA T^TAA
Tth111I 2483 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2043 TCTAGA T^CTAGA
XcmI 3084 CCAGCCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3434 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccII 150 CGCG CG^CG
553 CGCG
AcsI 324 GAATTC [GA]^AATT[CT]
784 GAATTT
Alw44I 271 GTGCAC G^TGCAC
2919 GTGCAC
AlwI 2227 GATCC GGATC(4/5)
2844 GATCC
Aor51HI 698 AGCGCT AGC^GCT
1016 AGCGCT
ApaLI 271 GTGCAC G^TGCAC
2919 GTGCAC
ApoI 324 GAATTC [GA]^AATT[CT]
784 GAATTT
AvrII 1198 CCTAGG C^CTAGG
3456 CCTAGG
BlnI 1198 CCTAGG C^CTAGG
3456 CCTAGG
BlpI 36 GCTCAGC GC^T[ACGT]AGC
927 GCTCAGC
Bpu1102I 36 GCTCAGC GC^T[ACGT]AGC
927 GCTCAGC
BsaWI 1044 ACCGGA [AT]^CCGG[AT]
3350 TCCGGA
BsmI 2292 GCATTC GAATGC(1/-1)
3652 GCATTC
BstUI 150 CGCG CG^CG
553 CGCG
BstXI 824 CCAACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1934 CCAGTGGTATGG
CelII 36 GCTCAGC GC^T[ACGT]AGC
927 GCTCAGC
DraIII 2645 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2667 CACGGGGTG
DrdI 355 GACAAGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1116 GACAGCATGGTC
Eco47III 698 AGCGCT AGC^GCT
1016 AGCGCT
MaeII 3167 ACGT A^CGT
3425 ACGT
MslI 1348 CACATCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2520 CATCACTGTG
MvnI 150 CGCG CG^CG
553 CGCG
PstI 2431 CTGCAG CTGCA^G
2715 CTGCAG
SphI 1320 GCATGC GCATG^C
2532 GCATGC
TfiI 1138 GAATC G^A[AT]TC
3204 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StuI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI